Rights statement: This is the author’s version of a work that was accepted for publication in Journal of Hazardous Materials. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Journal of Hazardous Materials, 419, 2021 DOI: 10.1016/j.jhazmat.2021.126471
Accepted author manuscript, 1.68 MB, PDF document
Available under license: CC BY-NC-ND
Final published version
Research output: Contribution to Journal/Magazine › Journal article › peer-review
Research output: Contribution to Journal/Magazine › Journal article › peer-review
}
TY - JOUR
T1 - Evaluating the simulated toxicities of metal mixtures and hydrocarbons using the alkane degrading bioreporter Acinetobacter baylyi ADPWH_recA
AU - Li, H.
AU - Yang, Y.
AU - Zhang, D.
AU - Li, Y.
AU - Zhang, H.
AU - Luo, J.
AU - Jones, K.C.
N1 - This is the author’s version of a work that was accepted for publication in Journal of Hazardous Materials. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Journal of Hazardous Materials, 419, 2021 DOI: 10.1016/j.jhazmat.2021.126471
PY - 2021/10/5
Y1 - 2021/10/5
N2 - Oil spillages lead to the formation of hydrocarbon and metal mixtures possessing effects on alkane-degrading bacteria that are responsible for the bioremediation of oil-contaminated soils and waters. Studies of bacterial responses to the mixture of petroleum and metal can inform appropriate strategies for bioremediation. We employed a luminescent bioreporter Acinetobacter baylyi ADPWH_recA with alkane degradation capability to evaluate the combined effects from heavy metals (Cd, Pb and Cu) and alkanes (dodecane, tetradecane, hexadecane and octadecane). Bioluminescent ratios of ADPWH_recA in single Cd or Pb treatments ranged from 0.25 to 1.98, indicating both genotoxicity and cytotoxicity of these two metals, while ratios <1.0 postexposure to Cu showed its cytotoxic impacts on ADPWH_recA bioreporter. Metal mixtures exhibited enhanced antagonistic effects (Ti > 4.0) determined by the Toxic Unit model. With 100 mg/L alkane, the morbidity of ADPWH-recA reduced to <20%, showing the inhibition of alkanes on Cd toxicity. Exposed to the metal mixture containing 10 mg/L Cu, the weak binding affinity of Cu with alkanes contributed to a high morbidity of > 85% in ADPWH_recA cells. This study provides a new way to understand the toxicity of mixture contaminants, which can help to optimize treatment efficiencies of bacterial remediation for oil contamination.
AB - Oil spillages lead to the formation of hydrocarbon and metal mixtures possessing effects on alkane-degrading bacteria that are responsible for the bioremediation of oil-contaminated soils and waters. Studies of bacterial responses to the mixture of petroleum and metal can inform appropriate strategies for bioremediation. We employed a luminescent bioreporter Acinetobacter baylyi ADPWH_recA with alkane degradation capability to evaluate the combined effects from heavy metals (Cd, Pb and Cu) and alkanes (dodecane, tetradecane, hexadecane and octadecane). Bioluminescent ratios of ADPWH_recA in single Cd or Pb treatments ranged from 0.25 to 1.98, indicating both genotoxicity and cytotoxicity of these two metals, while ratios <1.0 postexposure to Cu showed its cytotoxic impacts on ADPWH_recA bioreporter. Metal mixtures exhibited enhanced antagonistic effects (Ti > 4.0) determined by the Toxic Unit model. With 100 mg/L alkane, the morbidity of ADPWH-recA reduced to <20%, showing the inhibition of alkanes on Cd toxicity. Exposed to the metal mixture containing 10 mg/L Cu, the weak binding affinity of Cu with alkanes contributed to a high morbidity of > 85% in ADPWH_recA cells. This study provides a new way to understand the toxicity of mixture contaminants, which can help to optimize treatment efficiencies of bacterial remediation for oil contamination.
KW - Bioavailability
KW - Biosensor
KW - Mixture toxicity
KW - Toxic unit model
KW - Bacteria
KW - Binding energy
KW - Biochemistry
KW - Biodegradation
KW - Bioremediation
KW - Biosensors
KW - Biotechnology
KW - Cytotoxicity
KW - Diseases
KW - Heavy metals
KW - Paraffins
KW - Soil pollution
KW - Acinetobacters
KW - Contaminated water
KW - Degrading bacteria
KW - Hydrocarbons mixtures
KW - Metal mixtures
KW - Oil contaminated soil
KW - Oil spillage
KW - Soil and water
KW - Mixtures
KW - Acinetobacter baylyi
KW - Bacteria (microorganisms)
U2 - 10.1016/j.jhazmat.2021.126471
DO - 10.1016/j.jhazmat.2021.126471
M3 - Journal article
VL - 419
JO - Journal of Hazardous Materials
JF - Journal of Hazardous Materials
SN - 0304-3894
M1 - 126471
ER -