TY - JOUR

T1 - Evaluation of deletions in 7q11.2 and 8p12-p21 as prognostic indicators of tumour development following molar pregnancy

AU - Burke, Beverley

AU - Sebire, Neil J.

AU - Moss, Jill

AU - Hodges, Matt

AU - Seckl, Michael J.

AU - Newlands, Edward S.

AU - Fisher, Rosemary A.

PY - 2006/11

Y1 - 2006/11

N2 - ObjectivesPrevious studies have identified loss of chromosomal regions 7p12–q11.2 and 8p12–p21 in choriocarcinoma suggesting that suppressor genes involved in tumour development may be located within these regions. Our objectives were to refine the regions of loss and evaluate these deletions as prognostic indicators of trophoblastic tumour development following molar pregnancy.MethodsFluorescent microsatellite genotyping was used to perform deletion mapping in a series of thirty-nine gestational trophoblastic tumours (GTT) including both choriocarcinoma and placental site trophoblastic tumours.ResultsSignificant loss of heterozygosity (LOH) was found for both regions in GTT that originated in non-molar pregnancies. Although no common interval of loss was found in those GTT with LOH for the 7q11.2 region, for the 8p12–p21 locus, markers D8S1731 and NEFL defined a minimal region of loss in all tumours showing LOH. However, complete LOH of either region occurred in only a minority of tumours (20%; chromosome 7: 24%; chromosome 8) suggesting that loss of neither region is likely to be a primary event in the development of GTT. This was further supported by the observation that no deletions were found in either region for the fourteen GTT that followed complete molar pregnancies.ConclusionsWhile we have defined a minimal interval in 8p12–p21 in which tumour suppressor genes involved in GTT are likely to be located, the data suggest that deletions in 7q11.2 or 8p12–p21 are unlikely to be useful prognostic indicators in the management of patients with molar pregnancies.

AB - ObjectivesPrevious studies have identified loss of chromosomal regions 7p12–q11.2 and 8p12–p21 in choriocarcinoma suggesting that suppressor genes involved in tumour development may be located within these regions. Our objectives were to refine the regions of loss and evaluate these deletions as prognostic indicators of trophoblastic tumour development following molar pregnancy.MethodsFluorescent microsatellite genotyping was used to perform deletion mapping in a series of thirty-nine gestational trophoblastic tumours (GTT) including both choriocarcinoma and placental site trophoblastic tumours.ResultsSignificant loss of heterozygosity (LOH) was found for both regions in GTT that originated in non-molar pregnancies. Although no common interval of loss was found in those GTT with LOH for the 7q11.2 region, for the 8p12–p21 locus, markers D8S1731 and NEFL defined a minimal region of loss in all tumours showing LOH. However, complete LOH of either region occurred in only a minority of tumours (20%; chromosome 7: 24%; chromosome 8) suggesting that loss of neither region is likely to be a primary event in the development of GTT. This was further supported by the observation that no deletions were found in either region for the fourteen GTT that followed complete molar pregnancies.ConclusionsWhile we have defined a minimal interval in 8p12–p21 in which tumour suppressor genes involved in GTT are likely to be located, the data suggest that deletions in 7q11.2 or 8p12–p21 are unlikely to be useful prognostic indicators in the management of patients with molar pregnancies.

KW - Gestational trophoblastic tumour

KW - Hydatidiform mole

KW - Chromosome 7q

KW - Chromosome 8p

U2 - 10.1016/j.ygyno.2006.04.015

DO - 10.1016/j.ygyno.2006.04.015

M3 - Journal article

VL - 103

SP - 642

EP - 648

JO - Gynecologic Oncology

JF - Gynecologic Oncology

SN - 0090-8258

IS - 2

ER -