Final published version
Licence: CC BY-NC-ND
Research output: Contribution to Journal/Magazine › Journal article › peer-review
Research output: Contribution to Journal/Magazine › Journal article › peer-review
}
TY - JOUR
T1 - Evaluation of methods to estimate production, biomass and turnover of ectomycorrhizal mycelium in forests soils - A review
AU - Wallander, H.
AU - Ekblad, A.
AU - Godbold, D. L.
AU - Johnson, D.
AU - Bahr, A.
AU - Baldrian, P.
AU - Bjork, R. G.
AU - Kieliszewska-Rokicka, B.
AU - Kjoller, R.
AU - Kraigher, H.
AU - Plassard, C.
AU - Rudawska, M.
PY - 2013/2/28
Y1 - 2013/2/28
N2 - Mycorrhizal fungi constitute a considerable sink for carbon in most ecosystems. This carbon is used for building extensive mycelial networks in the soil as well as for metabolic activity related to nutrient uptake. A number of methods have been developed recently to quantify production, standing biomass and turnover of extramatrical mycorrhizal mycelia (EMM) in the field. These methods include minirhizotrons, in-growth mesh bags and cores, and indirect measurements of EMM based on classification of ectomycorrhizal fungi into exploration types. Here we review the state of the art of this methodology and discuss how it can be developed and applied most effectively in the field. Furthermore, we also discuss different ways to quantify fungal biomass based on biomarkers such as chitin, ergosterol and PLFAs, as well as molecular methods, such as qPCR. The evidence thus far indicates that mycorrhizal fungi are key components of microbial biomass in many ecosystems. We highlight the need to extend the application of current methods to focus on a greater range of habitats and mycorrhizal types enabling incorporation of mycorrhizal fungal biomass and turnover into biogeochemical cycling models.
AB - Mycorrhizal fungi constitute a considerable sink for carbon in most ecosystems. This carbon is used for building extensive mycelial networks in the soil as well as for metabolic activity related to nutrient uptake. A number of methods have been developed recently to quantify production, standing biomass and turnover of extramatrical mycorrhizal mycelia (EMM) in the field. These methods include minirhizotrons, in-growth mesh bags and cores, and indirect measurements of EMM based on classification of ectomycorrhizal fungi into exploration types. Here we review the state of the art of this methodology and discuss how it can be developed and applied most effectively in the field. Furthermore, we also discuss different ways to quantify fungal biomass based on biomarkers such as chitin, ergosterol and PLFAs, as well as molecular methods, such as qPCR. The evidence thus far indicates that mycorrhizal fungi are key components of microbial biomass in many ecosystems. We highlight the need to extend the application of current methods to focus on a greater range of habitats and mycorrhizal types enabling incorporation of mycorrhizal fungal biomass and turnover into biogeochemical cycling models.
KW - Chitin
KW - Exploration type
KW - Ergosterol
KW - Extramatrical mycelium
KW - In-growth bag
KW - Minirhizotron
KW - PLFA
KW - Rhizomorphs
KW - Sampling design
KW - Turnover rates
U2 - 10.1016/j.soilbio.2012.08.027
DO - 10.1016/j.soilbio.2012.08.027
M3 - Journal article
VL - 57
SP - 1034
EP - 1047
JO - Soil Biology and Biochemistry
JF - Soil Biology and Biochemistry
SN - 0038-0717
ER -