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Evidence for sodium metasilicate receptors on the human osteoblast cell surface; spatial localization and binding properties

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Evidence for sodium metasilicate receptors on the human osteoblast cell surface; spatial localization and binding properties. / Vere, Kelly-Ann; Richens, Joanna L.; Lane, Jordan S. et al.
In: Molecular Membrane Biology, Vol. 30, No. 8, 23.12.2013, p. 386-393.

Research output: Contribution to Journal/Magazine › Journal article › peer-review

Harvard

Vere, K-A, Richens, JL, Lane, JS, Harris, HJ, Duggan, J & O'Shea, P 2013, 'Evidence for sodium metasilicate receptors on the human osteoblast cell surface; spatial localization and binding properties', Molecular Membrane Biology, vol. 30, no. 8, pp. 386-393. https://doi.org/10.3109/09687688.2013.843031

APA

Vere, K-A., Richens, J. L., Lane, J. S., Harris, H. J., Duggan, J., & O'Shea, P. (2013). Evidence for sodium metasilicate receptors on the human osteoblast cell surface; spatial localization and binding properties. Molecular Membrane Biology, 30(8), 386-393. https://doi.org/10.3109/09687688.2013.843031

Vancouver

Vere K-A, Richens JL, Lane JS, Harris HJ, Duggan J, O'Shea P. Evidence for sodium metasilicate receptors on the human osteoblast cell surface; spatial localization and binding properties. Molecular Membrane Biology. 2013 Dec 23;30(8):386-393. Epub 2013 Oct 23. doi: 10.3109/09687688.2013.843031

Author

Vere, Kelly-Ann ; Richens, Joanna L. ; Lane, Jordan S. et al. / Evidence for sodium metasilicate receptors on the human osteoblast cell surface; spatial localization and binding properties. In: Molecular Membrane Biology. 2013 ; Vol. 30, No. 8. pp. 386-393.

Bibtex

@article{8258139016c748e18a13290e87c79ba7,
title = "Evidence for sodium metasilicate receptors on the human osteoblast cell surface; spatial localization and binding properties",
abstract = "We report details of the interaction of sodium metasilicate with osteoblast cellular membranes using Fluoresceinphosphatidylethanolamine (FPE) as a fluorescent indicator of membrane interactions. Fluorescence imaging studies of the FPE-based indicator system revealed areas of localized binding that would be consistent with the presence of a structure with 'receptor-like' properties. From these results, it seems unlikely that silica binds 'non-specifically' to the osteoblast surface. Moreover, the receptors are localized into membrane domains. Such regions of the cell membrane could well be structures such as 'rafts' or other such localized domains within the membrane. The binding profile of silica with the osteoblast cell surface takes place with all the characteristics of a receptor-mediated process best represented by a cooperativity (sigmoidal) binding model with a Hill coefficient of 3.6.",
author = "Kelly-Ann Vere and Richens, {Joanna L.} and Lane, {Jordan S.} and Harris, {Helen J.} and James Duggan and Paul O'Shea",
year = "2013",
month = dec,
day = "23",
doi = "10.3109/09687688.2013.843031",
language = "English",
volume = "30",
pages = "386--393",
journal = "Molecular Membrane Biology",
issn = "0968-7688",
publisher = "Informa Healthcare",
number = "8",

}

RIS

TY - JOUR

T1 - Evidence for sodium metasilicate receptors on the human osteoblast cell surface; spatial localization and binding properties

AU - Vere, Kelly-Ann

AU - Richens, Joanna L.

AU - Lane, Jordan S.

AU - Harris, Helen J.

AU - Duggan, James

AU - O'Shea, Paul

PY - 2013/12/23

Y1 - 2013/12/23

N2 - We report details of the interaction of sodium metasilicate with osteoblast cellular membranes using Fluoresceinphosphatidylethanolamine (FPE) as a fluorescent indicator of membrane interactions. Fluorescence imaging studies of the FPE-based indicator system revealed areas of localized binding that would be consistent with the presence of a structure with 'receptor-like' properties. From these results, it seems unlikely that silica binds 'non-specifically' to the osteoblast surface. Moreover, the receptors are localized into membrane domains. Such regions of the cell membrane could well be structures such as 'rafts' or other such localized domains within the membrane. The binding profile of silica with the osteoblast cell surface takes place with all the characteristics of a receptor-mediated process best represented by a cooperativity (sigmoidal) binding model with a Hill coefficient of 3.6.

AB - We report details of the interaction of sodium metasilicate with osteoblast cellular membranes using Fluoresceinphosphatidylethanolamine (FPE) as a fluorescent indicator of membrane interactions. Fluorescence imaging studies of the FPE-based indicator system revealed areas of localized binding that would be consistent with the presence of a structure with 'receptor-like' properties. From these results, it seems unlikely that silica binds 'non-specifically' to the osteoblast surface. Moreover, the receptors are localized into membrane domains. Such regions of the cell membrane could well be structures such as 'rafts' or other such localized domains within the membrane. The binding profile of silica with the osteoblast cell surface takes place with all the characteristics of a receptor-mediated process best represented by a cooperativity (sigmoidal) binding model with a Hill coefficient of 3.6.

U2 - 10.3109/09687688.2013.843031

DO - 10.3109/09687688.2013.843031

M3 - Journal article

C2 - 24147954

VL - 30

SP - 386

EP - 393

JO - Molecular Membrane Biology

JF - Molecular Membrane Biology

SN - 0968-7688

IS - 8

ER -