Home > Research > Publications & Outputs > Evidence incriminating midges (Diptera: Ceratop...
View graph of relations

Evidence incriminating midges (Diptera: Ceratopogonidae) as potential vectors of Leishmania in Australia

Research output: Contribution to journalJournal articlepeer-review

Published

Standard

Evidence incriminating midges (Diptera: Ceratopogonidae) as potential vectors of Leishmania in Australia. / Dougall, Annette M; Alexander, Bruce; Holt, Deborah C; Harris, Tegan; Sultan, Amal H; Bates, Paul A; Rose, Karrie; Walton, Shelley F.

In: International Journal for Parasitology, Vol. 41, No. 5, 04.2011, p. 571-579.

Research output: Contribution to journalJournal articlepeer-review

Harvard

Dougall, AM, Alexander, B, Holt, DC, Harris, T, Sultan, AH, Bates, PA, Rose, K & Walton, SF 2011, 'Evidence incriminating midges (Diptera: Ceratopogonidae) as potential vectors of Leishmania in Australia', International Journal for Parasitology, vol. 41, no. 5, pp. 571-579. https://doi.org/10.1016/j.ijpara.2010.12.008

APA

Dougall, A. M., Alexander, B., Holt, D. C., Harris, T., Sultan, A. H., Bates, P. A., Rose, K., & Walton, S. F. (2011). Evidence incriminating midges (Diptera: Ceratopogonidae) as potential vectors of Leishmania in Australia. International Journal for Parasitology, 41(5), 571-579. https://doi.org/10.1016/j.ijpara.2010.12.008

Vancouver

Dougall AM, Alexander B, Holt DC, Harris T, Sultan AH, Bates PA et al. Evidence incriminating midges (Diptera: Ceratopogonidae) as potential vectors of Leishmania in Australia. International Journal for Parasitology. 2011 Apr;41(5):571-579. https://doi.org/10.1016/j.ijpara.2010.12.008

Author

Dougall, Annette M ; Alexander, Bruce ; Holt, Deborah C ; Harris, Tegan ; Sultan, Amal H ; Bates, Paul A ; Rose, Karrie ; Walton, Shelley F. / Evidence incriminating midges (Diptera: Ceratopogonidae) as potential vectors of Leishmania in Australia. In: International Journal for Parasitology. 2011 ; Vol. 41, No. 5. pp. 571-579.

Bibtex

@article{8f46d1a7711d4a3e83479168a5e63091,
title = "Evidence incriminating midges (Diptera: Ceratopogonidae) as potential vectors of Leishmania in Australia",
abstract = "The first autochthonous Leishmania infection in Australia was reported by Rose et al. (2004) and the parasite was characterised as a unique species. The host was the red kangaroo (Macropus rufus) but the transmitting vector was unknown. To incriminate the biological vector, insect trapping by a variety of methods was undertaken at two field sites of known Leishmania transmission. Collected sand flies were identified to species level and were screened for Leishmania DNA using a semi-quantitative real-time PCR. Collections revealed four species of sand fly, with a predominance of the reptile biter Sergentomyia queenslandi (Hill). However, no Leishmania-positive flies were detected. Therefore, alternative vectors were investigated for infection, giving startling results. Screening revealed that an undescribed species of day-feeding midge, subgenus Forcipomyia (Lasiohelea) Kieffer, had a prevalence of up to 15% for Leishmania DNA, with high parasitemia in some individuals. Manual gut dissections confirmed the presence of promastigotes and in some midges material similar to promastigote secretory gel, including parasites with metacyclic-like morphology. Parasites were cultured from infected midges and sequence analysis of the Leishmania RNA polymerase subunit II gene confirmed infections were identical to the original isolated Leishmania sp. Phylogenetic analysis revealed the closest known species to be Leishmania enriettii, with this and the Australian species confirmed as members of Leishmania sensu stricto. Collectively the results strongly suggest that the day-feeding midge (F. (Lasiohelea) sp. 1) is a potential biological vector of Leishmania in northern Australia, which is to our knowledge the first evidence of a vector other than a phlebotomine sand fly anywhere in the world. These findings have considerable implications in the understanding of the Leishmania life cycle worldwide.",
keywords = "Animals, Australia, Ceratopogonidae, Insect Vectors, Leishmania, Leishmaniasis, Macropodidae, Molecular Sequence Data, Phylogeny",
author = "Dougall, {Annette M} and Bruce Alexander and Holt, {Deborah C} and Tegan Harris and Sultan, {Amal H} and Bates, {Paul A} and Karrie Rose and Walton, {Shelley F}",
note = "Copyright {\textcopyright} 2011 Australian Society for Parasitology Inc. All rights reserved.",
year = "2011",
month = apr,
doi = "10.1016/j.ijpara.2010.12.008",
language = "English",
volume = "41",
pages = "571--579",
journal = "International Journal for Parasitology",
issn = "0020-7519",
publisher = "Elsevier Limited",
number = "5",

}

RIS

TY - JOUR

T1 - Evidence incriminating midges (Diptera: Ceratopogonidae) as potential vectors of Leishmania in Australia

AU - Dougall, Annette M

AU - Alexander, Bruce

AU - Holt, Deborah C

AU - Harris, Tegan

AU - Sultan, Amal H

AU - Bates, Paul A

AU - Rose, Karrie

AU - Walton, Shelley F

N1 - Copyright © 2011 Australian Society for Parasitology Inc. All rights reserved.

PY - 2011/4

Y1 - 2011/4

N2 - The first autochthonous Leishmania infection in Australia was reported by Rose et al. (2004) and the parasite was characterised as a unique species. The host was the red kangaroo (Macropus rufus) but the transmitting vector was unknown. To incriminate the biological vector, insect trapping by a variety of methods was undertaken at two field sites of known Leishmania transmission. Collected sand flies were identified to species level and were screened for Leishmania DNA using a semi-quantitative real-time PCR. Collections revealed four species of sand fly, with a predominance of the reptile biter Sergentomyia queenslandi (Hill). However, no Leishmania-positive flies were detected. Therefore, alternative vectors were investigated for infection, giving startling results. Screening revealed that an undescribed species of day-feeding midge, subgenus Forcipomyia (Lasiohelea) Kieffer, had a prevalence of up to 15% for Leishmania DNA, with high parasitemia in some individuals. Manual gut dissections confirmed the presence of promastigotes and in some midges material similar to promastigote secretory gel, including parasites with metacyclic-like morphology. Parasites were cultured from infected midges and sequence analysis of the Leishmania RNA polymerase subunit II gene confirmed infections were identical to the original isolated Leishmania sp. Phylogenetic analysis revealed the closest known species to be Leishmania enriettii, with this and the Australian species confirmed as members of Leishmania sensu stricto. Collectively the results strongly suggest that the day-feeding midge (F. (Lasiohelea) sp. 1) is a potential biological vector of Leishmania in northern Australia, which is to our knowledge the first evidence of a vector other than a phlebotomine sand fly anywhere in the world. These findings have considerable implications in the understanding of the Leishmania life cycle worldwide.

AB - The first autochthonous Leishmania infection in Australia was reported by Rose et al. (2004) and the parasite was characterised as a unique species. The host was the red kangaroo (Macropus rufus) but the transmitting vector was unknown. To incriminate the biological vector, insect trapping by a variety of methods was undertaken at two field sites of known Leishmania transmission. Collected sand flies were identified to species level and were screened for Leishmania DNA using a semi-quantitative real-time PCR. Collections revealed four species of sand fly, with a predominance of the reptile biter Sergentomyia queenslandi (Hill). However, no Leishmania-positive flies were detected. Therefore, alternative vectors were investigated for infection, giving startling results. Screening revealed that an undescribed species of day-feeding midge, subgenus Forcipomyia (Lasiohelea) Kieffer, had a prevalence of up to 15% for Leishmania DNA, with high parasitemia in some individuals. Manual gut dissections confirmed the presence of promastigotes and in some midges material similar to promastigote secretory gel, including parasites with metacyclic-like morphology. Parasites were cultured from infected midges and sequence analysis of the Leishmania RNA polymerase subunit II gene confirmed infections were identical to the original isolated Leishmania sp. Phylogenetic analysis revealed the closest known species to be Leishmania enriettii, with this and the Australian species confirmed as members of Leishmania sensu stricto. Collectively the results strongly suggest that the day-feeding midge (F. (Lasiohelea) sp. 1) is a potential biological vector of Leishmania in northern Australia, which is to our knowledge the first evidence of a vector other than a phlebotomine sand fly anywhere in the world. These findings have considerable implications in the understanding of the Leishmania life cycle worldwide.

KW - Animals

KW - Australia

KW - Ceratopogonidae

KW - Insect Vectors

KW - Leishmania

KW - Leishmaniasis

KW - Macropodidae

KW - Molecular Sequence Data

KW - Phylogeny

UR - http://www.scopus.com/inward/record.url?scp=79952695132&partnerID=8YFLogxK

U2 - 10.1016/j.ijpara.2010.12.008

DO - 10.1016/j.ijpara.2010.12.008

M3 - Journal article

C2 - 21251914

VL - 41

SP - 571

EP - 579

JO - International Journal for Parasitology

JF - International Journal for Parasitology

SN - 0020-7519

IS - 5

ER -