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Evidence that RNA editing modulates splice site selection in the 5-HT2C receptor gene

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Evidence that RNA editing modulates splice site selection in the 5-HT2C receptor gene. / Flomen, Rachel; Knight, Joanne; Sham, Pak et al.

In: Nucleic Acids Research, Vol. 32, No. 7, 01.04.2004, p. 2113-2122.

Research output: Contribution to Journal/MagazineJournal articlepeer-review

Harvard

Flomen, R, Knight, J, Sham, P, Kerwin, R & Makoff, A 2004, 'Evidence that RNA editing modulates splice site selection in the 5-HT2C receptor gene', Nucleic Acids Research, vol. 32, no. 7, pp. 2113-2122. https://doi.org/10.1093/nar/gkh536

APA

Flomen, R., Knight, J., Sham, P., Kerwin, R., & Makoff, A. (2004). Evidence that RNA editing modulates splice site selection in the 5-HT2C receptor gene. Nucleic Acids Research, 32(7), 2113-2122. https://doi.org/10.1093/nar/gkh536

Vancouver

Flomen R, Knight J, Sham P, Kerwin R, Makoff A. Evidence that RNA editing modulates splice site selection in the 5-HT2C receptor gene. Nucleic Acids Research. 2004 Apr 1;32(7):2113-2122. doi: 10.1093/nar/gkh536

Author

Flomen, Rachel ; Knight, Joanne ; Sham, Pak et al. / Evidence that RNA editing modulates splice site selection in the 5-HT2C receptor gene. In: Nucleic Acids Research. 2004 ; Vol. 32, No. 7. pp. 2113-2122.

Bibtex

@article{b31d031405f54c6ca297a088c2690a04,
title = "Evidence that RNA editing modulates splice site selection in the 5-HT2C receptor gene",
abstract = "Adenosine to inosine editing of mRNA from the human 5-HT2C receptor gene (HTR2C) occurs at five exonic positions (A-E) in a stable stem-loop that includes the normal 5' splice site of intron 5 and is flanked by two alternative splice sites. Using in vitro editing, we identified a novel editing site (F) located in the intronic part of the stem-loop and demonstrated editing at this site in human brain. We have shown that in cell culture, base substitutions to mimic editing at different combinations of the six sites profoundly affect relative splicing at the normal and the upstream alternative splice site, but splicing at the downstream alternative splice site was consistently rare. Editing combinations in different splice variants from human brain were determined and are consistent with the effects of editing on splicing observed in cell culture. As RNA editing usually occurs close to exon/intron boundaries, this is likely to be a general phenomenon and suggests an important novel role for RNA editing.",
keywords = "Aged, Aged, 80 and over, Alternative Splicing, Animals, Base Sequence, Binding Sites, Brain, COS Cells, Cell Line, Cell Line, Tumor, Female, Humans, Introns, Male, Models, Genetic, Molecular Sequence Data, Mutation, PC12 Cells, RNA, RNA Editing, Rats, Receptor, Serotonin, 5-HT2C",
author = "Rachel Flomen and Joanne Knight and Pak Sham and Robert Kerwin and Andrew Makoff",
year = "2004",
month = apr,
day = "1",
doi = "10.1093/nar/gkh536",
language = "English",
volume = "32",
pages = "2113--2122",
journal = "Nucleic Acids Research",
issn = "0305-1048",
publisher = "Oxford University Press",
number = "7",

}

RIS

TY - JOUR

T1 - Evidence that RNA editing modulates splice site selection in the 5-HT2C receptor gene

AU - Flomen, Rachel

AU - Knight, Joanne

AU - Sham, Pak

AU - Kerwin, Robert

AU - Makoff, Andrew

PY - 2004/4/1

Y1 - 2004/4/1

N2 - Adenosine to inosine editing of mRNA from the human 5-HT2C receptor gene (HTR2C) occurs at five exonic positions (A-E) in a stable stem-loop that includes the normal 5' splice site of intron 5 and is flanked by two alternative splice sites. Using in vitro editing, we identified a novel editing site (F) located in the intronic part of the stem-loop and demonstrated editing at this site in human brain. We have shown that in cell culture, base substitutions to mimic editing at different combinations of the six sites profoundly affect relative splicing at the normal and the upstream alternative splice site, but splicing at the downstream alternative splice site was consistently rare. Editing combinations in different splice variants from human brain were determined and are consistent with the effects of editing on splicing observed in cell culture. As RNA editing usually occurs close to exon/intron boundaries, this is likely to be a general phenomenon and suggests an important novel role for RNA editing.

AB - Adenosine to inosine editing of mRNA from the human 5-HT2C receptor gene (HTR2C) occurs at five exonic positions (A-E) in a stable stem-loop that includes the normal 5' splice site of intron 5 and is flanked by two alternative splice sites. Using in vitro editing, we identified a novel editing site (F) located in the intronic part of the stem-loop and demonstrated editing at this site in human brain. We have shown that in cell culture, base substitutions to mimic editing at different combinations of the six sites profoundly affect relative splicing at the normal and the upstream alternative splice site, but splicing at the downstream alternative splice site was consistently rare. Editing combinations in different splice variants from human brain were determined and are consistent with the effects of editing on splicing observed in cell culture. As RNA editing usually occurs close to exon/intron boundaries, this is likely to be a general phenomenon and suggests an important novel role for RNA editing.

KW - Aged

KW - Aged, 80 and over

KW - Alternative Splicing

KW - Animals

KW - Base Sequence

KW - Binding Sites

KW - Brain

KW - COS Cells

KW - Cell Line

KW - Cell Line, Tumor

KW - Female

KW - Humans

KW - Introns

KW - Male

KW - Models, Genetic

KW - Molecular Sequence Data

KW - Mutation

KW - PC12 Cells

KW - RNA

KW - RNA Editing

KW - Rats

KW - Receptor, Serotonin, 5-HT2C

U2 - 10.1093/nar/gkh536

DO - 10.1093/nar/gkh536

M3 - Journal article

C2 - 15087490

VL - 32

SP - 2113

EP - 2122

JO - Nucleic Acids Research

JF - Nucleic Acids Research

SN - 0305-1048

IS - 7

ER -