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Experimental infection of Leishmania (Mundinia) martiniquensis in BALB/c mice and Syrian golden hamsters

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Experimental infection of Leishmania (Mundinia) martiniquensis in BALB/c mice and Syrian golden hamsters. / Intakhan, N.; Chanmol, W.; Kongkaew, A.; Somboon, P.; Bates, M.D.; Bates, P.A.; Jariyapan, N.

In: Parasitology Research, Vol. 119, 01.09.2020, p. 3041-3051.

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Intakhan, N. ; Chanmol, W. ; Kongkaew, A. ; Somboon, P. ; Bates, M.D. ; Bates, P.A. ; Jariyapan, N. / Experimental infection of Leishmania (Mundinia) martiniquensis in BALB/c mice and Syrian golden hamsters. In: Parasitology Research. 2020 ; Vol. 119. pp. 3041-3051.

Bibtex

@article{56840b9187fc4098bfc95a00012b6b52,
title = "Experimental infection of Leishmania (Mundinia) martiniquensis in BALB/c mice and Syrian golden hamsters",
abstract = "Our objective was to investigate clinical progression, presence of parasites and DNAs, parasite loads, and histological alterations in BALB/c mice and Syrian golden hamsters after intraperitoneal inoculation with Leishmania (Mundinia) martiniquensis promastigotes with a goal to choosing an appropriate animal model for visceral leishmaniasis. Infections were monitored for 16 weeks. Infected BALB/c mice were asymptomatic during the infection course. Parasite DNAs were detected in the liver at week 8 of infection, followed by clearance in most animals at week 16; whereas in the spleen, parasite DNAs were detected until week 16. These results are correlated to those obtained measuring parasite loads in both organs. No parasite DNA and no alteration in the bone marrow were observed indicating that no dissemination occurred. These results suggest the control of visceralization of L. martiniquensis by BALB/c mice. In hamsters, weight loss, cachexia, and fatigue were observed after week 11. Leishmania martiniquensis parasites were observed in tissue smears of the liver, spleen, and bone marrow by week 16. Parasite loads correlated with those from the presence of parasites and DNAs in the examined tissues. Alterations in the liver with nuclear destruction and cytoplasmic degeneration of infected hepatocytes, presence of inflammatory infiltrates, necrosis of hepatocytes, and changes in splenic architecture and reduction and deformation of white pulp in the spleen were noted. These results indicate a chronic form of visceral leishmaniasis indicating that the hamster is a suitable animal model for the study of pathological features of chronic visceral leishmaniasis caused by L. martiniquensis. {\textcopyright} 2020, Springer-Verlag GmbH Germany, part of Springer Nature.",
keywords = "animal model, BALB/c mouse, Leishmania martiniquensis, Mundinia, Syrian golden hamster",
author = "N. Intakhan and W. Chanmol and A. Kongkaew and P. Somboon and M.D. Bates and P.A. Bates and N. Jariyapan",
note = "The final publication is available at Springer via https://doi.org/10.1007/s00436-020-06842-w",
year = "2020",
month = sep,
day = "1",
doi = "10.1007/s00436-020-06842-w",
language = "English",
volume = "119",
pages = "3041--3051",
journal = "Parasitology Research",
issn = "0044-3255",
publisher = "Springer Verlag",

}

RIS

TY - JOUR

T1 - Experimental infection of Leishmania (Mundinia) martiniquensis in BALB/c mice and Syrian golden hamsters

AU - Intakhan, N.

AU - Chanmol, W.

AU - Kongkaew, A.

AU - Somboon, P.

AU - Bates, M.D.

AU - Bates, P.A.

AU - Jariyapan, N.

N1 - The final publication is available at Springer via https://doi.org/10.1007/s00436-020-06842-w

PY - 2020/9/1

Y1 - 2020/9/1

N2 - Our objective was to investigate clinical progression, presence of parasites and DNAs, parasite loads, and histological alterations in BALB/c mice and Syrian golden hamsters after intraperitoneal inoculation with Leishmania (Mundinia) martiniquensis promastigotes with a goal to choosing an appropriate animal model for visceral leishmaniasis. Infections were monitored for 16 weeks. Infected BALB/c mice were asymptomatic during the infection course. Parasite DNAs were detected in the liver at week 8 of infection, followed by clearance in most animals at week 16; whereas in the spleen, parasite DNAs were detected until week 16. These results are correlated to those obtained measuring parasite loads in both organs. No parasite DNA and no alteration in the bone marrow were observed indicating that no dissemination occurred. These results suggest the control of visceralization of L. martiniquensis by BALB/c mice. In hamsters, weight loss, cachexia, and fatigue were observed after week 11. Leishmania martiniquensis parasites were observed in tissue smears of the liver, spleen, and bone marrow by week 16. Parasite loads correlated with those from the presence of parasites and DNAs in the examined tissues. Alterations in the liver with nuclear destruction and cytoplasmic degeneration of infected hepatocytes, presence of inflammatory infiltrates, necrosis of hepatocytes, and changes in splenic architecture and reduction and deformation of white pulp in the spleen were noted. These results indicate a chronic form of visceral leishmaniasis indicating that the hamster is a suitable animal model for the study of pathological features of chronic visceral leishmaniasis caused by L. martiniquensis. © 2020, Springer-Verlag GmbH Germany, part of Springer Nature.

AB - Our objective was to investigate clinical progression, presence of parasites and DNAs, parasite loads, and histological alterations in BALB/c mice and Syrian golden hamsters after intraperitoneal inoculation with Leishmania (Mundinia) martiniquensis promastigotes with a goal to choosing an appropriate animal model for visceral leishmaniasis. Infections were monitored for 16 weeks. Infected BALB/c mice were asymptomatic during the infection course. Parasite DNAs were detected in the liver at week 8 of infection, followed by clearance in most animals at week 16; whereas in the spleen, parasite DNAs were detected until week 16. These results are correlated to those obtained measuring parasite loads in both organs. No parasite DNA and no alteration in the bone marrow were observed indicating that no dissemination occurred. These results suggest the control of visceralization of L. martiniquensis by BALB/c mice. In hamsters, weight loss, cachexia, and fatigue were observed after week 11. Leishmania martiniquensis parasites were observed in tissue smears of the liver, spleen, and bone marrow by week 16. Parasite loads correlated with those from the presence of parasites and DNAs in the examined tissues. Alterations in the liver with nuclear destruction and cytoplasmic degeneration of infected hepatocytes, presence of inflammatory infiltrates, necrosis of hepatocytes, and changes in splenic architecture and reduction and deformation of white pulp in the spleen were noted. These results indicate a chronic form of visceral leishmaniasis indicating that the hamster is a suitable animal model for the study of pathological features of chronic visceral leishmaniasis caused by L. martiniquensis. © 2020, Springer-Verlag GmbH Germany, part of Springer Nature.

KW - animal model

KW - BALB/c mouse

KW - Leishmania martiniquensis

KW - Mundinia

KW - Syrian golden hamster

U2 - 10.1007/s00436-020-06842-w

DO - 10.1007/s00436-020-06842-w

M3 - Journal article

VL - 119

SP - 3041

EP - 3051

JO - Parasitology Research

JF - Parasitology Research

SN - 0044-3255

ER -