Home > Research > Publications & Outputs > Exploitation of immunofluorescence for the quan...

Research

Links

Text available via DOI:

Keywords

View graph of relations

Exploitation of immunofluorescence for the quantification and characterisation of small numbers of Pasteuria endospores.

Research output: Contribution to Journal/Magazine › Journal article › peer-review

Published

Standard

Exploitation of immunofluorescence for the quantification and characterisation of small numbers of Pasteuria endospores. / Costa, Sofia R.; Kerry, Brian R.; Bardgett, Richard D. et al.
In: FEMS Microbiology Ecology, Vol. 58, No. 3, 12.2006, p. 593-600.

Research output: Contribution to Journal/Magazine › Journal article › peer-review

Harvard

Costa, SR, Kerry, BR, Bardgett, RD & Davies, KG 2006, 'Exploitation of immunofluorescence for the quantification and characterisation of small numbers of Pasteuria endospores.', FEMS Microbiology Ecology, vol. 58, no. 3, pp. 593-600. https://doi.org/10.1111/j.1574-6941.2006.00188.x

APA

Costa, S. R., Kerry, B. R., Bardgett, R. D., & Davies, K. G. (2006). Exploitation of immunofluorescence for the quantification and characterisation of small numbers of Pasteuria endospores. FEMS Microbiology Ecology, 58(3), 593-600. https://doi.org/10.1111/j.1574-6941.2006.00188.x

Vancouver

Costa SR, Kerry BR, Bardgett RD, Davies KG. Exploitation of immunofluorescence for the quantification and characterisation of small numbers of Pasteuria endospores. FEMS Microbiology Ecology. 2006 Dec;58(3):593-600. doi: 10.1111/j.1574-6941.2006.00188.x

Author

Costa, Sofia R. ; Kerry, Brian R. ; Bardgett, Richard D. et al. / Exploitation of immunofluorescence for the quantification and characterisation of small numbers of Pasteuria endospores. In: FEMS Microbiology Ecology. 2006 ; Vol. 58, No. 3. pp. 593-600.

Bibtex

@article{8f72fc7ea2f2475f8d9a3951e1032fc7,
title = "Exploitation of immunofluorescence for the quantification and characterisation of small numbers of Pasteuria endospores.",
abstract = "The Pasteuria group of endospore-forming bacteria has been studied as a biocontrol agent of plant-parasitic nematodes. Techniques have been developed for its detection and quantification in soil samples, and these mainly focus on observations of endospore attachment to nematodes. Characterization of Pasteuria populations has recently been performed with DNA-based techniques, which usually require the extraction of large numbers of spores. We describe a simple immunological method for the quantification and characterization of Pasteuria populations. Bayesian statistics were used to determine an extraction efficiency of 43% and a threshold of detection of 210 endospores g−1 sand. This provided a robust means of estimating numbers of endospores in small-volume samples from a natural system. Based on visual assessment of endospore fluorescence, a quantitative method was developed to characterize endospore populations, which were shown to vary according to their host.",
keywords = "population characterization • threshold of detection • Pasteuria penetrans • Pasteuria ramosa • bayesian statistics • sand dune",
author = "Costa, {Sofia R.} and Kerry, {Brian R.} and Bardgett, {Richard D.} and Davies, {Keith G.}",
year = "2006",
month = dec,
doi = "10.1111/j.1574-6941.2006.00188.x",
language = "English",
volume = "58",
pages = "593--600",
journal = "FEMS Microbiology Ecology",
issn = "0168-6496",
publisher = "Wiley-Blackwell",
number = "3",

}

RIS

TY - JOUR

T1 - Exploitation of immunofluorescence for the quantification and characterisation of small numbers of Pasteuria endospores.

AU - Costa, Sofia R.

AU - Kerry, Brian R.

AU - Bardgett, Richard D.

AU - Davies, Keith G.

PY - 2006/12

Y1 - 2006/12

N2 - The Pasteuria group of endospore-forming bacteria has been studied as a biocontrol agent of plant-parasitic nematodes. Techniques have been developed for its detection and quantification in soil samples, and these mainly focus on observations of endospore attachment to nematodes. Characterization of Pasteuria populations has recently been performed with DNA-based techniques, which usually require the extraction of large numbers of spores. We describe a simple immunological method for the quantification and characterization of Pasteuria populations. Bayesian statistics were used to determine an extraction efficiency of 43% and a threshold of detection of 210 endospores g−1 sand. This provided a robust means of estimating numbers of endospores in small-volume samples from a natural system. Based on visual assessment of endospore fluorescence, a quantitative method was developed to characterize endospore populations, which were shown to vary according to their host.

AB - The Pasteuria group of endospore-forming bacteria has been studied as a biocontrol agent of plant-parasitic nematodes. Techniques have been developed for its detection and quantification in soil samples, and these mainly focus on observations of endospore attachment to nematodes. Characterization of Pasteuria populations has recently been performed with DNA-based techniques, which usually require the extraction of large numbers of spores. We describe a simple immunological method for the quantification and characterization of Pasteuria populations. Bayesian statistics were used to determine an extraction efficiency of 43% and a threshold of detection of 210 endospores g−1 sand. This provided a robust means of estimating numbers of endospores in small-volume samples from a natural system. Based on visual assessment of endospore fluorescence, a quantitative method was developed to characterize endospore populations, which were shown to vary according to their host.

KW - population characterization • threshold of detection • Pasteuria penetrans • Pasteuria ramosa • bayesian statistics • sand dune

U2 - 10.1111/j.1574-6941.2006.00188.x

DO - 10.1111/j.1574-6941.2006.00188.x

M3 - Journal article

VL - 58

SP - 593

EP - 600

JO - FEMS Microbiology Ecology

JF - FEMS Microbiology Ecology

SN - 0168-6496

IS - 3

ER -