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Expression of cysteine proteinases by metacyclic promastigotes of Leishmania mexicana

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Expression of cysteine proteinases by metacyclic promastigotes of Leishmania mexicana. / Bates, P A; Robertson, C D; Coombs, G H.

In: Journal of Eukaryotic Microbiology, Vol. 41, No. 3, 05.1994, p. 199-203.

Research output: Contribution to Journal/MagazineJournal articlepeer-review

Harvard

Bates, PA, Robertson, CD & Coombs, GH 1994, 'Expression of cysteine proteinases by metacyclic promastigotes of Leishmania mexicana', Journal of Eukaryotic Microbiology, vol. 41, no. 3, pp. 199-203. https://doi.org/10.1111/j.1550-7408.1994.tb01497.x

APA

Bates, P. A., Robertson, C. D., & Coombs, G. H. (1994). Expression of cysteine proteinases by metacyclic promastigotes of Leishmania mexicana. Journal of Eukaryotic Microbiology, 41(3), 199-203. https://doi.org/10.1111/j.1550-7408.1994.tb01497.x

Vancouver

Author

Bates, P A ; Robertson, C D ; Coombs, G H. / Expression of cysteine proteinases by metacyclic promastigotes of Leishmania mexicana. In: Journal of Eukaryotic Microbiology. 1994 ; Vol. 41, No. 3. pp. 199-203.

Bibtex

@article{0a68ef6ac22440028ba373e8968f2b81,
title = "Expression of cysteine proteinases by metacyclic promastigotes of Leishmania mexicana",
abstract = "The expression of cysteine proteinases by metacyclic promastigotes of Leishmania mexicana was investigated using gelatin polyacrylamide gel electrophoresis. Two prominent bands were detected which distinguished metacyclics from multiplicative promastigotes, lacking detectable cysteine proteinase activity, and amastigotes, with a distinct banding pattern composed of multiple enzymes. A correlation between relative activity of the metacyclic-specific bands and the prevalence of metacyclics was found both during the growth cycle in vitro as metacyclogenesis occurred, and by comparison of stationary phase populations from consecutive subpassages in vitro. Irreversible inhibition of the metacyclic activities using N-benzyloxycarbonyl-phenylalanyl-alanyl diazomethane did not inhibit metacyclic to amastigote transformation in vitro. These activities provide a useful biochemical marker for the metacyclic promastigotes of L. mexicana.",
keywords = "Amastigote, metacyclogenesis , transformation",
author = "Bates, {P A} and Robertson, {C D} and Coombs, {G H}",
year = "1994",
month = may,
doi = "10.1111/j.1550-7408.1994.tb01497.x",
language = "English",
volume = "41",
pages = "199--203",
journal = "Journal of Eukaryotic Microbiology",
issn = "1066-5234",
publisher = "Wiley-Blackwell",
number = "3",

}

RIS

TY - JOUR

T1 - Expression of cysteine proteinases by metacyclic promastigotes of Leishmania mexicana

AU - Bates, P A

AU - Robertson, C D

AU - Coombs, G H

PY - 1994/5

Y1 - 1994/5

N2 - The expression of cysteine proteinases by metacyclic promastigotes of Leishmania mexicana was investigated using gelatin polyacrylamide gel electrophoresis. Two prominent bands were detected which distinguished metacyclics from multiplicative promastigotes, lacking detectable cysteine proteinase activity, and amastigotes, with a distinct banding pattern composed of multiple enzymes. A correlation between relative activity of the metacyclic-specific bands and the prevalence of metacyclics was found both during the growth cycle in vitro as metacyclogenesis occurred, and by comparison of stationary phase populations from consecutive subpassages in vitro. Irreversible inhibition of the metacyclic activities using N-benzyloxycarbonyl-phenylalanyl-alanyl diazomethane did not inhibit metacyclic to amastigote transformation in vitro. These activities provide a useful biochemical marker for the metacyclic promastigotes of L. mexicana.

AB - The expression of cysteine proteinases by metacyclic promastigotes of Leishmania mexicana was investigated using gelatin polyacrylamide gel electrophoresis. Two prominent bands were detected which distinguished metacyclics from multiplicative promastigotes, lacking detectable cysteine proteinase activity, and amastigotes, with a distinct banding pattern composed of multiple enzymes. A correlation between relative activity of the metacyclic-specific bands and the prevalence of metacyclics was found both during the growth cycle in vitro as metacyclogenesis occurred, and by comparison of stationary phase populations from consecutive subpassages in vitro. Irreversible inhibition of the metacyclic activities using N-benzyloxycarbonyl-phenylalanyl-alanyl diazomethane did not inhibit metacyclic to amastigote transformation in vitro. These activities provide a useful biochemical marker for the metacyclic promastigotes of L. mexicana.

KW - Amastigote

KW - metacyclogenesis

KW - transformation

U2 - 10.1111/j.1550-7408.1994.tb01497.x

DO - 10.1111/j.1550-7408.1994.tb01497.x

M3 - Journal article

C2 - 8049682

VL - 41

SP - 199

EP - 203

JO - Journal of Eukaryotic Microbiology

JF - Journal of Eukaryotic Microbiology

SN - 1066-5234

IS - 3

ER -