Research output: Contribution to Journal/Magazine › Journal article › peer-review
Research output: Contribution to Journal/Magazine › Journal article › peer-review
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TY - JOUR
T1 - Expression of D7 and D7-related proteins in the salivary glands of the human malaria mosquito Anopheles stephensi
AU - Suwan, N
AU - Wilkinson, M C
AU - Crampton, J M
AU - Bates, P A
PY - 2002/6
Y1 - 2002/6
N2 - Full-length cDNA clones encoding D7 (AnsD7) and D7-related (AnsD7r1) secreted salivary gland proteins were isolated from Anopheles stephensi. Corresponding proteins were separated by SDS-PAGE and analysed by N-terminal sequencing, which also identified a second D7-related protein (AnsD7r2). AnsD7 encodes a protein of 37 kDa, AnsD7r1 of 18 kDa, and AnsD7r2 of 16 kDa. Polyclonal antibodies against recombinant AnsD7 showed immunological cross-reactivity with the D7-related proteins, and alignment demonstrated sequence similarity between the C-terminal region of AnsD7 and the D7-related proteins. AnsD7, AnsD7r1 and AnsD7r2 were major female-specific salivary gland proteins, and Western blotting, immunohistochemistry and immunogold labelling demonstrated expression was predominantly in the secretory cavities of the distal-lateral and median lobes. Expression and localization of D7 and D7-related proteins was similar in Plasmodium berghei-infected and uninfected mosquitoes.
AB - Full-length cDNA clones encoding D7 (AnsD7) and D7-related (AnsD7r1) secreted salivary gland proteins were isolated from Anopheles stephensi. Corresponding proteins were separated by SDS-PAGE and analysed by N-terminal sequencing, which also identified a second D7-related protein (AnsD7r2). AnsD7 encodes a protein of 37 kDa, AnsD7r1 of 18 kDa, and AnsD7r2 of 16 kDa. Polyclonal antibodies against recombinant AnsD7 showed immunological cross-reactivity with the D7-related proteins, and alignment demonstrated sequence similarity between the C-terminal region of AnsD7 and the D7-related proteins. AnsD7, AnsD7r1 and AnsD7r2 were major female-specific salivary gland proteins, and Western blotting, immunohistochemistry and immunogold labelling demonstrated expression was predominantly in the secretory cavities of the distal-lateral and median lobes. Expression and localization of D7 and D7-related proteins was similar in Plasmodium berghei-infected and uninfected mosquitoes.
KW - Anopheles stephensi
KW - salivary glands
KW - mosquito
KW - Malaria
U2 - 10.1046/j.1365-2583.2002.00329.x
DO - 10.1046/j.1365-2583.2002.00329.x
M3 - Journal article
C2 - 12000641
VL - 11
SP - 223
EP - 232
JO - Insect Molecular Biology
JF - Insect Molecular Biology
SN - 1365-2583
IS - 3
ER -