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Expression of GIMAP1, a GTPase of the immunity-associated protein family, is not up-regulated in malaria

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Expression of GIMAP1, a GTPase of the immunity-associated protein family, is not up-regulated in malaria. / Saunders, Amy; Lamb, Tracey; Pascall, John et al.
In: Malaria Journal, Vol. 8, 53, 02.04.2009.

Research output: Contribution to Journal/Magazine › Journal article › peer-review

Harvard

Saunders, A, Lamb, T, Pascall, J, Hutchings, A, Dion, C, Carter, C, Hepburn, L, Langhorne, J & Butcher, GW 2009, 'Expression of GIMAP1, a GTPase of the immunity-associated protein family, is not up-regulated in malaria', Malaria Journal, vol. 8, 53. https://doi.org/10.1186/1475-2875-8-53

APA

Saunders, A., Lamb, T., Pascall, J., Hutchings, A., Dion, C., Carter, C., Hepburn, L., Langhorne, J., & Butcher, G. W. (2009). Expression of GIMAP1, a GTPase of the immunity-associated protein family, is not up-regulated in malaria. Malaria Journal, 8, Article 53. https://doi.org/10.1186/1475-2875-8-53

Vancouver

Saunders A, Lamb T, Pascall J, Hutchings A, Dion C, Carter C et al. Expression of GIMAP1, a GTPase of the immunity-associated protein family, is not up-regulated in malaria. Malaria Journal. 2009 Apr 2;8:53. doi: 10.1186/1475-2875-8-53

Author

Saunders, Amy ; Lamb, Tracey ; Pascall, John et al. / Expression of GIMAP1, a GTPase of the immunity-associated protein family, is not up-regulated in malaria. In: Malaria Journal. 2009 ; Vol. 8.

Bibtex

@article{6b410aaa49944b4b8a2f302ae15a48c2,
title = "Expression of GIMAP1, a GTPase of the immunity-associated protein family, is not up-regulated in malaria",
abstract = "BACKGROUND: GIMAP (GTPase of the immunity-associated protein family) proteins are a family of putative GTPases believed to be regulators of cell death in lymphomyeloid cells. GIMAP1 was the first reported member of this gene family, identified as a gene up-regulated at the RNA level in the spleens of mice infected with the malarial parasite, Plasmodium chabaudi.METHODS: A monoclonal antibody against mouse GIMAP1 was developed and was used to analyse the expression of the endogenous protein in tissues of normal mice and in defined sub-populations of cells prepared from lymphoid tissues using flow cytometry. It was also used to assess the expression of GIMAP1 protein after infection and/or immunization of mice with P. chabaudi. Real-time PCR analysis was employed to measure the expression of GIMAP1 for comparison with the protein level analysis.RESULTS: GIMAP1 protein expression was detected in all lineages of lymphocytes (T, B, NK), in F4/80+ splenic macrophages and in some lymphoid cell lines. Additional evidence is presented suggesting that the strong expression by mature B cells of GIMAP1 and other GIMAP genes and proteins seen in mice may be a species-dependent characteristic. Unexpectedly, no increase was found in the expression of GIMAP1 in P. chabaudi infected mice at either the mRNA or protein level, and this remained so despite applying a number of variations to the protocol.CONCLUSION: The model of up-regulation of GIMAP1 in response to infection/immunization with P. chabaudi is not a robustly reproducible experimental system. The GIMAP1 protein is widely expressed in lymphoid cells, with an interesting increase in expression in the later stages of B cell development. Alternative approaches will be required to define the functional role of this GTPase in immune cells.",
keywords = "Animals, Antibodies, Monoclonal, Blotting, Western, Cell Line, Flow Cytometry, GTP Phosphohydrolases/metabolism, GTP-Binding Proteins/metabolism, Lymphocytes/metabolism, Malaria/immunology, Male, Membrane Proteins/metabolism, Mice, Mice, Inbred C57BL, Plasmodium chabaudi/immunology, Polymerase Chain Reaction, Spleen/cytology, Up-Regulation",
author = "Amy Saunders and Tracey Lamb and John Pascall and Amanda Hutchings and Carine Dion and Christine Carter and Lucy Hepburn and Jean Langhorne and Butcher, {Geoffrey W}",
year = "2009",
month = apr,
day = "2",
doi = "10.1186/1475-2875-8-53",
language = "English",
volume = "8",
journal = "Malaria Journal",
issn = "1475-2875",
publisher = "BioMed Central",

}

RIS

TY - JOUR

T1 - Expression of GIMAP1, a GTPase of the immunity-associated protein family, is not up-regulated in malaria

AU - Saunders, Amy

AU - Lamb, Tracey

AU - Pascall, John

AU - Hutchings, Amanda

AU - Dion, Carine

AU - Carter, Christine

AU - Hepburn, Lucy

AU - Langhorne, Jean

AU - Butcher, Geoffrey W

PY - 2009/4/2

Y1 - 2009/4/2

N2 - BACKGROUND: GIMAP (GTPase of the immunity-associated protein family) proteins are a family of putative GTPases believed to be regulators of cell death in lymphomyeloid cells. GIMAP1 was the first reported member of this gene family, identified as a gene up-regulated at the RNA level in the spleens of mice infected with the malarial parasite, Plasmodium chabaudi.METHODS: A monoclonal antibody against mouse GIMAP1 was developed and was used to analyse the expression of the endogenous protein in tissues of normal mice and in defined sub-populations of cells prepared from lymphoid tissues using flow cytometry. It was also used to assess the expression of GIMAP1 protein after infection and/or immunization of mice with P. chabaudi. Real-time PCR analysis was employed to measure the expression of GIMAP1 for comparison with the protein level analysis.RESULTS: GIMAP1 protein expression was detected in all lineages of lymphocytes (T, B, NK), in F4/80+ splenic macrophages and in some lymphoid cell lines. Additional evidence is presented suggesting that the strong expression by mature B cells of GIMAP1 and other GIMAP genes and proteins seen in mice may be a species-dependent characteristic. Unexpectedly, no increase was found in the expression of GIMAP1 in P. chabaudi infected mice at either the mRNA or protein level, and this remained so despite applying a number of variations to the protocol.CONCLUSION: The model of up-regulation of GIMAP1 in response to infection/immunization with P. chabaudi is not a robustly reproducible experimental system. The GIMAP1 protein is widely expressed in lymphoid cells, with an interesting increase in expression in the later stages of B cell development. Alternative approaches will be required to define the functional role of this GTPase in immune cells.

AB - BACKGROUND: GIMAP (GTPase of the immunity-associated protein family) proteins are a family of putative GTPases believed to be regulators of cell death in lymphomyeloid cells. GIMAP1 was the first reported member of this gene family, identified as a gene up-regulated at the RNA level in the spleens of mice infected with the malarial parasite, Plasmodium chabaudi.METHODS: A monoclonal antibody against mouse GIMAP1 was developed and was used to analyse the expression of the endogenous protein in tissues of normal mice and in defined sub-populations of cells prepared from lymphoid tissues using flow cytometry. It was also used to assess the expression of GIMAP1 protein after infection and/or immunization of mice with P. chabaudi. Real-time PCR analysis was employed to measure the expression of GIMAP1 for comparison with the protein level analysis.RESULTS: GIMAP1 protein expression was detected in all lineages of lymphocytes (T, B, NK), in F4/80+ splenic macrophages and in some lymphoid cell lines. Additional evidence is presented suggesting that the strong expression by mature B cells of GIMAP1 and other GIMAP genes and proteins seen in mice may be a species-dependent characteristic. Unexpectedly, no increase was found in the expression of GIMAP1 in P. chabaudi infected mice at either the mRNA or protein level, and this remained so despite applying a number of variations to the protocol.CONCLUSION: The model of up-regulation of GIMAP1 in response to infection/immunization with P. chabaudi is not a robustly reproducible experimental system. The GIMAP1 protein is widely expressed in lymphoid cells, with an interesting increase in expression in the later stages of B cell development. Alternative approaches will be required to define the functional role of this GTPase in immune cells.

KW - Animals

KW - Antibodies, Monoclonal

KW - Blotting, Western

KW - Cell Line

KW - Flow Cytometry

KW - GTP Phosphohydrolases/metabolism

KW - GTP-Binding Proteins/metabolism

KW - Lymphocytes/metabolism

KW - Malaria/immunology

KW - Male

KW - Membrane Proteins/metabolism

KW - Mice

KW - Mice, Inbred C57BL

KW - Plasmodium chabaudi/immunology

KW - Polymerase Chain Reaction

KW - Spleen/cytology

KW - Up-Regulation

U2 - 10.1186/1475-2875-8-53

DO - 10.1186/1475-2875-8-53

M3 - Journal article

C2 - 19338674

VL - 8

JO - Malaria Journal

JF - Malaria Journal

SN - 1475-2875

M1 - 53

ER -