Research output: Contribution to Journal/Magazine › Journal article › peer-review
Research output: Contribution to Journal/Magazine › Journal article › peer-review
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TY - JOUR
T1 - Expression profiling of the response of Arabidopsis thaliana to methanol stimulation
AU - Downie, Andrew
AU - Miyazaki, Saori
AU - Bohnert, Hans
AU - John, Philip
AU - Coleman, Julian
AU - Parry, Martin
AU - Haslam, Richard
PY - 2004/8/1
Y1 - 2004/8/1
N2 - In order to obtain information about the metabolism of methanol in plants, gene expression in response to methanol stimulation was analysed in leaves of Arabidopsis thaliana through the use of a 26,090 element oligonucleotide microarray. Following confirmation of data and the application of selection criteria, a total of 484 (1.9%) transcripts were shown to be regulated in response to a 10% methanol application. Samples were taken at 1, 24 and 72 h. Of the total identified transcripts, only 20 were shared between all three time points of which only two were down-regulated. Based on functional category analysis of these genes, at 1 h, the largest categories were metabolism, cell communication/signal transduction processes, defence genes associated with transcription and aspects of RNA processing; a few related to cell division and growth; but none were associated with photosynthesis. At 24 and 72 h, the number and overall proportion of regulated transcripts associated with metabolism increased further. Direct measurement of anthocyanin and flavonoid content confirmed that methanol-regulated transcripts corresponded to flavonoid pathways. Within the functional class metabolism, genes encoding detoxification proteins represented by far the most strongly regulated group. These included, cytochrome P450's, glucosyl transferases and members of the ABC transporter family. It is concluded that exposure to methanol affects the expression of hundreds of genes and that multiple detoxification and signalling pathways are activated.
AB - In order to obtain information about the metabolism of methanol in plants, gene expression in response to methanol stimulation was analysed in leaves of Arabidopsis thaliana through the use of a 26,090 element oligonucleotide microarray. Following confirmation of data and the application of selection criteria, a total of 484 (1.9%) transcripts were shown to be regulated in response to a 10% methanol application. Samples were taken at 1, 24 and 72 h. Of the total identified transcripts, only 20 were shared between all three time points of which only two were down-regulated. Based on functional category analysis of these genes, at 1 h, the largest categories were metabolism, cell communication/signal transduction processes, defence genes associated with transcription and aspects of RNA processing; a few related to cell division and growth; but none were associated with photosynthesis. At 24 and 72 h, the number and overall proportion of regulated transcripts associated with metabolism increased further. Direct measurement of anthocyanin and flavonoid content confirmed that methanol-regulated transcripts corresponded to flavonoid pathways. Within the functional class metabolism, genes encoding detoxification proteins represented by far the most strongly regulated group. These included, cytochrome P450's, glucosyl transferases and members of the ABC transporter family. It is concluded that exposure to methanol affects the expression of hundreds of genes and that multiple detoxification and signalling pathways are activated.
KW - Anthocyanins
KW - Arabidopsis thaliana
KW - Detoxification
KW - Gene expression profile
KW - Methanol
KW - Microarray
KW - One-carbon units
UR - http://www.scopus.com/inward/record.url?scp=4544336639&partnerID=8YFLogxK
U2 - 10.1016/j.phytochem.2004.07.006
DO - 10.1016/j.phytochem.2004.07.006
M3 - Journal article
C2 - 15381001
AN - SCOPUS:4544336639
VL - 65
SP - 2305
EP - 2316
JO - Phytochemistry
JF - Phytochemistry
SN - 0031-9422
IS - 16
ER -