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Filamentous proteophosphoglycan secreted by Leishmania promastigotes forms gel-like three-dimensional networks that obstruct the digestive tract of infected sandfly vectors

Research output: Contribution to Journal/Magazine › Journal article › peer-review

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Filamentous proteophosphoglycan secreted by Leishmania promastigotes forms gel-like three-dimensional networks that obstruct the digestive tract of infected sandfly vectors. / Stierhof, Y D; Bates, P A; Jacobson, R L et al.
In: European Journal of Cell Biology, Vol. 78, No. 10, 10.1999, p. 675-689.

Research output: Contribution to Journal/Magazine › Journal article › peer-review

Harvard

Stierhof, YD, Bates, PA, Jacobson, RL, Rogers, ME, Schlein, Y, Handman, E & Ilg, T 1999, 'Filamentous proteophosphoglycan secreted by Leishmania promastigotes forms gel-like three-dimensional networks that obstruct the digestive tract of infected sandfly vectors', European Journal of Cell Biology, vol. 78, no. 10, pp. 675-689. https://doi.org/10.1016/S0171-9335(99)80036-3

APA

Stierhof, Y. D., Bates, P. A., Jacobson, R. L., Rogers, M. E., Schlein, Y., Handman, E., & Ilg, T. (1999). Filamentous proteophosphoglycan secreted by Leishmania promastigotes forms gel-like three-dimensional networks that obstruct the digestive tract of infected sandfly vectors. European Journal of Cell Biology, 78(10), 675-689. https://doi.org/10.1016/S0171-9335(99)80036-3

Vancouver

Stierhof YD, Bates PA, Jacobson RL, Rogers ME, Schlein Y, Handman E et al. Filamentous proteophosphoglycan secreted by Leishmania promastigotes forms gel-like three-dimensional networks that obstruct the digestive tract of infected sandfly vectors. European Journal of Cell Biology. 1999 Oct;78(10):675-689. doi: 10.1016/S0171-9335(99)80036-3

Author

Stierhof, Y D ; Bates, P A ; Jacobson, R L et al. / Filamentous proteophosphoglycan secreted by Leishmania promastigotes forms gel-like three-dimensional networks that obstruct the digestive tract of infected sandfly vectors. In: European Journal of Cell Biology. 1999 ; Vol. 78, No. 10. pp. 675-689.

Bibtex

@article{c1b305a1e5f1426b843b3534ae30c24e,
title = "Filamentous proteophosphoglycan secreted by Leishmania promastigotes forms gel-like three-dimensional networks that obstruct the digestive tract of infected sandfly vectors",
abstract = "Development of Leishmania parasites in the digestive tract of their sandfly vectors involves several morphological transformations from the intracellular mammalian amastigote via a succession of free and gut wall-attached promastigote stages to the infective metacyclic promastigotes. At the foregut midgut transition of Leishmania-infected sandflies a gel-like plug of unknown origin and composition is formed, which contains high numbers of parasites, that occludes the gut lumen and which may be responsible for the often observed inability of infected sandflies to draw blood. This {"}blocked fly{"} phenotype has been linked to efficient transmission of infectious metacyclic promastigotes from the vector to the mammalian host. We show by immunofluorescence and immunoelectron microscopy on two Leishmania/sandfly vector combinations (Leishmania mexicana/Lutzomyia longipalpis and L. major/Phlebotomus papatasi) that the gel-like mass is formed mainly by a parasite-derived mucin-like filamentous proteophosphoglycan (fPPG) whereas the Leishmania polymeric secreted acid phosphatase (SAP) is not a major component of this plug. fPPG forms a dense three-dimensional network of filaments which engulf the promastigote cell bodies in a gel-like mass. We propose that the continuous secretion of fPPG by promastigotes in the sandfly gut, that causes plug formation, is an important factor for the efficient transmission to the mammalian host.",
keywords = "Immunoelectron microscopy, proteophosphoglycan, secreted acid phosphatase, sandfly",
author = "Stierhof, {Y D} and Bates, {P A} and Jacobson, {R L} and Rogers, {M E} and Y Schlein and E Handman and T Ilg",
year = "1999",
month = oct,
doi = "10.1016/S0171-9335(99)80036-3",
language = "English",
volume = "78",
pages = "675--689",
journal = "European Journal of Cell Biology",
issn = "0171-9335",
publisher = "Urban und Fischer Verlag GmbH und Co. KG",
number = "10",

}

RIS

TY - JOUR

T1 - Filamentous proteophosphoglycan secreted by Leishmania promastigotes forms gel-like three-dimensional networks that obstruct the digestive tract of infected sandfly vectors

AU - Stierhof, Y D

AU - Bates, P A

AU - Jacobson, R L

AU - Rogers, M E

AU - Schlein, Y

AU - Handman, E

AU - Ilg, T

PY - 1999/10

Y1 - 1999/10

N2 - Development of Leishmania parasites in the digestive tract of their sandfly vectors involves several morphological transformations from the intracellular mammalian amastigote via a succession of free and gut wall-attached promastigote stages to the infective metacyclic promastigotes. At the foregut midgut transition of Leishmania-infected sandflies a gel-like plug of unknown origin and composition is formed, which contains high numbers of parasites, that occludes the gut lumen and which may be responsible for the often observed inability of infected sandflies to draw blood. This "blocked fly" phenotype has been linked to efficient transmission of infectious metacyclic promastigotes from the vector to the mammalian host. We show by immunofluorescence and immunoelectron microscopy on two Leishmania/sandfly vector combinations (Leishmania mexicana/Lutzomyia longipalpis and L. major/Phlebotomus papatasi) that the gel-like mass is formed mainly by a parasite-derived mucin-like filamentous proteophosphoglycan (fPPG) whereas the Leishmania polymeric secreted acid phosphatase (SAP) is not a major component of this plug. fPPG forms a dense three-dimensional network of filaments which engulf the promastigote cell bodies in a gel-like mass. We propose that the continuous secretion of fPPG by promastigotes in the sandfly gut, that causes plug formation, is an important factor for the efficient transmission to the mammalian host.

AB - Development of Leishmania parasites in the digestive tract of their sandfly vectors involves several morphological transformations from the intracellular mammalian amastigote via a succession of free and gut wall-attached promastigote stages to the infective metacyclic promastigotes. At the foregut midgut transition of Leishmania-infected sandflies a gel-like plug of unknown origin and composition is formed, which contains high numbers of parasites, that occludes the gut lumen and which may be responsible for the often observed inability of infected sandflies to draw blood. This "blocked fly" phenotype has been linked to efficient transmission of infectious metacyclic promastigotes from the vector to the mammalian host. We show by immunofluorescence and immunoelectron microscopy on two Leishmania/sandfly vector combinations (Leishmania mexicana/Lutzomyia longipalpis and L. major/Phlebotomus papatasi) that the gel-like mass is formed mainly by a parasite-derived mucin-like filamentous proteophosphoglycan (fPPG) whereas the Leishmania polymeric secreted acid phosphatase (SAP) is not a major component of this plug. fPPG forms a dense three-dimensional network of filaments which engulf the promastigote cell bodies in a gel-like mass. We propose that the continuous secretion of fPPG by promastigotes in the sandfly gut, that causes plug formation, is an important factor for the efficient transmission to the mammalian host.

KW - Immunoelectron microscopy

KW - proteophosphoglycan

KW - secreted acid phosphatase

KW - sandfly

U2 - 10.1016/S0171-9335(99)80036-3

DO - 10.1016/S0171-9335(99)80036-3

M3 - Journal article

C2 - 10569240

VL - 78

SP - 675

EP - 689

JO - European Journal of Cell Biology

JF - European Journal of Cell Biology

SN - 0171-9335

IS - 10

ER -