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First surveillance of SARS-CoV-2 and organic tracers in community wastewater during post lockdown in Chennai, South India: Methods, occurrence and concurrence

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First surveillance of SARS-CoV-2 and organic tracers in community wastewater during post lockdown in Chennai, South India: Methods, occurrence and concurrence. / Chakraborty, P.; Pasupuleti, M.; Jai Shankar, M.R. et al.
In: Science of the Total Environment, Vol. 778, 146252, 15.07.2021.

Research output: Contribution to Journal/Magazine › Journal article › peer-review

Harvard

Chakraborty, P, Pasupuleti, M, Jai Shankar, MR, Bharat, GK, Krishnasamy, S, Dasgupta, SC, Sarkar, SK & Jones, KC 2021, 'First surveillance of SARS-CoV-2 and organic tracers in community wastewater during post lockdown in Chennai, South India: Methods, occurrence and concurrence', Science of the Total Environment, vol. 778, 146252. https://doi.org/10.1016/j.scitotenv.2021.146252

APA

Chakraborty, P., Pasupuleti, M., Jai Shankar, M. R., Bharat, G. K., Krishnasamy, S., Dasgupta, S. C., Sarkar, S. K., & Jones, K. C. (2021). First surveillance of SARS-CoV-2 and organic tracers in community wastewater during post lockdown in Chennai, South India: Methods, occurrence and concurrence. Science of the Total Environment, 778, Article 146252. https://doi.org/10.1016/j.scitotenv.2021.146252

Vancouver

Chakraborty P, Pasupuleti M, Jai Shankar MR, Bharat GK, Krishnasamy S, Dasgupta SC et al. First surveillance of SARS-CoV-2 and organic tracers in community wastewater during post lockdown in Chennai, South India: Methods, occurrence and concurrence. Science of the Total Environment. 2021 Jul 15;778:146252. Epub 2021 Mar 6. doi: 10.1016/j.scitotenv.2021.146252

Author

Chakraborty, P. ; Pasupuleti, M. ; Jai Shankar, M.R. et al. / First surveillance of SARS-CoV-2 and organic tracers in community wastewater during post lockdown in Chennai, South India : Methods, occurrence and concurrence. In: Science of the Total Environment. 2021 ; Vol. 778.

Bibtex

@article{9d78db3b7b094b8f8bdee8e0e3b043e9,
title = "First surveillance of SARS-CoV-2 and organic tracers in community wastewater during post lockdown in Chennai, South India: Methods, occurrence and concurrence",
abstract = "Surveillance of SARS-CoV-2 and organic tracers (OTs) were conducted in the community wastewater of Chennai city and the suburbs, South India, during partial and post lockdown phases (August–September 2020) as a response to the coronavirus disease 2019 (COVID-19) pandemic. Wastewater samples were collected from four sewage treatment plants (STPs), five sewage pumping stations (SPSs) and at different time intervals from a suburban hospital wastewater (HWW). Four different methods of wastewater concentrations viz., composite (COM), supernatant (SUP), sediment (SED), and syringe filtration (SYR) were subjected to quantitative real time-polymerase chain reaction (qRT-PCR). Unlike HWW, STP inlet, sludge and SPS samples were found with higher loading of SARS-CoV-2 by SED followed by SUP method. Given the higher levels of dissolved and suspended solids in STPs and SPSs over HWW, we suspect that this enveloped virus might exhibit the tendency of higher partitioning in solid phase. Cycle threshold (Ct) values were < 30 in 50% of the HWW samples indicating higher viral load from the COVID-19 infected patients. In the STP outlets, a strict decline of biochemical oxygen demand, >95% removal of caffeine, and absence of viral copies reflect the efficiency of the treatment plants in Chennai city. Among the detected OTs, a combination of maximum dynamic range and high concurrence percentage was observed for caffeine and N1 gene of SARS-CoV-2. Hence, we suggest that caffeine can be used as an indicator for the removal of SARS-CoV-2 by STPs. Our predicted estimated number of cases are in line with the available clinical data from the catchments. Densely distributed population of the Koyambedu catchment could be partly responsible for the high proportion of estimated infected individuals during the study period. ",
keywords = "Chemical marker, RNA copies, SARS-CoV-2, Wastewater, Wastewater based epidemiology, Caffeine, Catchments, Plants (botany), Polymerase chain reaction, Population statistics, Runoff, Sewage treatment plants, Viruses, Wastewater treatment, Chemical markers, Chennai, Hospital wastewater, Organic tracers, RNA copy, Sewage pumping station, Sewage-treatment plants, South India, Coronavirus, Indicator indicator, SARS coronavirus",
author = "P. Chakraborty and M. Pasupuleti and {Jai Shankar}, M.R. and G.K. Bharat and S. Krishnasamy and S.C. Dasgupta and S.K. Sarkar and K.C. Jones",
year = "2021",
month = jul,
day = "15",
doi = "10.1016/j.scitotenv.2021.146252",
language = "English",
volume = "778",
journal = "Science of the Total Environment",
issn = "0048-9697",
publisher = "Elsevier Science B.V.",

}

RIS

TY - JOUR

T1 - First surveillance of SARS-CoV-2 and organic tracers in community wastewater during post lockdown in Chennai, South India

T2 - Methods, occurrence and concurrence

AU - Chakraborty, P.

AU - Pasupuleti, M.

AU - Jai Shankar, M.R.

AU - Bharat, G.K.

AU - Krishnasamy, S.

AU - Dasgupta, S.C.

AU - Sarkar, S.K.

AU - Jones, K.C.

PY - 2021/7/15

Y1 - 2021/7/15

N2 - Surveillance of SARS-CoV-2 and organic tracers (OTs) were conducted in the community wastewater of Chennai city and the suburbs, South India, during partial and post lockdown phases (August–September 2020) as a response to the coronavirus disease 2019 (COVID-19) pandemic. Wastewater samples were collected from four sewage treatment plants (STPs), five sewage pumping stations (SPSs) and at different time intervals from a suburban hospital wastewater (HWW). Four different methods of wastewater concentrations viz., composite (COM), supernatant (SUP), sediment (SED), and syringe filtration (SYR) were subjected to quantitative real time-polymerase chain reaction (qRT-PCR). Unlike HWW, STP inlet, sludge and SPS samples were found with higher loading of SARS-CoV-2 by SED followed by SUP method. Given the higher levels of dissolved and suspended solids in STPs and SPSs over HWW, we suspect that this enveloped virus might exhibit the tendency of higher partitioning in solid phase. Cycle threshold (Ct) values were < 30 in 50% of the HWW samples indicating higher viral load from the COVID-19 infected patients. In the STP outlets, a strict decline of biochemical oxygen demand, >95% removal of caffeine, and absence of viral copies reflect the efficiency of the treatment plants in Chennai city. Among the detected OTs, a combination of maximum dynamic range and high concurrence percentage was observed for caffeine and N1 gene of SARS-CoV-2. Hence, we suggest that caffeine can be used as an indicator for the removal of SARS-CoV-2 by STPs. Our predicted estimated number of cases are in line with the available clinical data from the catchments. Densely distributed population of the Koyambedu catchment could be partly responsible for the high proportion of estimated infected individuals during the study period. 

AB - Surveillance of SARS-CoV-2 and organic tracers (OTs) were conducted in the community wastewater of Chennai city and the suburbs, South India, during partial and post lockdown phases (August–September 2020) as a response to the coronavirus disease 2019 (COVID-19) pandemic. Wastewater samples were collected from four sewage treatment plants (STPs), five sewage pumping stations (SPSs) and at different time intervals from a suburban hospital wastewater (HWW). Four different methods of wastewater concentrations viz., composite (COM), supernatant (SUP), sediment (SED), and syringe filtration (SYR) were subjected to quantitative real time-polymerase chain reaction (qRT-PCR). Unlike HWW, STP inlet, sludge and SPS samples were found with higher loading of SARS-CoV-2 by SED followed by SUP method. Given the higher levels of dissolved and suspended solids in STPs and SPSs over HWW, we suspect that this enveloped virus might exhibit the tendency of higher partitioning in solid phase. Cycle threshold (Ct) values were < 30 in 50% of the HWW samples indicating higher viral load from the COVID-19 infected patients. In the STP outlets, a strict decline of biochemical oxygen demand, >95% removal of caffeine, and absence of viral copies reflect the efficiency of the treatment plants in Chennai city. Among the detected OTs, a combination of maximum dynamic range and high concurrence percentage was observed for caffeine and N1 gene of SARS-CoV-2. Hence, we suggest that caffeine can be used as an indicator for the removal of SARS-CoV-2 by STPs. Our predicted estimated number of cases are in line with the available clinical data from the catchments. Densely distributed population of the Koyambedu catchment could be partly responsible for the high proportion of estimated infected individuals during the study period. 

KW - Chemical marker

KW - RNA copies

KW - SARS-CoV-2

KW - Wastewater

KW - Wastewater based epidemiology

KW - Caffeine

KW - Catchments

KW - Plants (botany)

KW - Polymerase chain reaction

KW - Population statistics

KW - Runoff

KW - Sewage treatment plants

KW - Viruses

KW - Wastewater treatment

KW - Chemical markers

KW - Chennai

KW - Hospital wastewater

KW - Organic tracers

KW - RNA copy

KW - Sewage pumping station

KW - Sewage-treatment plants

KW - South India

KW - Coronavirus

KW - Indicator indicator

KW - SARS coronavirus

U2 - 10.1016/j.scitotenv.2021.146252

DO - 10.1016/j.scitotenv.2021.146252

M3 - Journal article

VL - 778

JO - Science of the Total Environment

JF - Science of the Total Environment

SN - 0048-9697

M1 - 146252

ER -