Final published version
Research output: Contribution to Journal/Magazine › Review article › peer-review
Research output: Contribution to Journal/Magazine › Review article › peer-review
}
TY - JOUR
T1 - Fluorescent probes and flow cytometry
T2 - New insights into environmental bacteriology
AU - Porter, Jonathan
AU - Deere, Daniel
AU - Pickup, Roger
AU - Edwards, Clive
PY - 1996/2/1
Y1 - 1996/2/1
N2 - Recent trends in flow cytometry have established new techniques in bacteriology. Advances in fluorescent dye technology complement these improvements, offering probes for a variety of cellular functions. Bacterial ecology requires the application of new techniques to help answer questions unanswerable by traditional methods alone. Here we review some aspects of how coupling the two technologies has enabled researchers to directly study individual bacterial cells, and revealed the complexity and heterogeneity present in both laboratory cultures and in environmental samples. Results are discussed with respect to viability analysis, stress induced changes, specific cell detection and cell sorting.
AB - Recent trends in flow cytometry have established new techniques in bacteriology. Advances in fluorescent dye technology complement these improvements, offering probes for a variety of cellular functions. Bacterial ecology requires the application of new techniques to help answer questions unanswerable by traditional methods alone. Here we review some aspects of how coupling the two technologies has enabled researchers to directly study individual bacterial cells, and revealed the complexity and heterogeneity present in both laboratory cultures and in environmental samples. Results are discussed with respect to viability analysis, stress induced changes, specific cell detection and cell sorting.
KW - Cell sorting
KW - Environmental bacteria
KW - Specific identification
KW - Viability assessment
U2 - 10.1002/(SICI)1097-0320(19960201)23:2<91::AID-CYTO1>3.0.CO;2-O
DO - 10.1002/(SICI)1097-0320(19960201)23:2<91::AID-CYTO1>3.0.CO;2-O
M3 - Review article
C2 - 8742166
AN - SCOPUS:0030064429
VL - 23
SP - 91
EP - 96
JO - Cytometry
JF - Cytometry
SN - 0196-4763
IS - 2
ER -