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Fluorescent probes and flow cytometry: New insights into environmental bacteriology

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Fluorescent probes and flow cytometry: New insights into environmental bacteriology. / Porter, Jonathan; Deere, Daniel; Pickup, Roger et al.
In: Cytometry, Vol. 23, No. 2, 01.02.1996, p. 91-96.

Research output: Contribution to Journal/MagazineReview articlepeer-review

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Porter J, Deere D, Pickup R, Edwards C. Fluorescent probes and flow cytometry: New insights into environmental bacteriology. Cytometry. 1996 Feb 1;23(2):91-96. doi: 10.1002/(SICI)1097-0320(19960201)23:2<91::AID-CYTO1>3.0.CO;2-O

Author

Porter, Jonathan ; Deere, Daniel ; Pickup, Roger et al. / Fluorescent probes and flow cytometry : New insights into environmental bacteriology. In: Cytometry. 1996 ; Vol. 23, No. 2. pp. 91-96.

Bibtex

@article{e55a34e00fe047c4b2a833d884477894,
title = "Fluorescent probes and flow cytometry: New insights into environmental bacteriology",
abstract = "Recent trends in flow cytometry have established new techniques in bacteriology. Advances in fluorescent dye technology complement these improvements, offering probes for a variety of cellular functions. Bacterial ecology requires the application of new techniques to help answer questions unanswerable by traditional methods alone. Here we review some aspects of how coupling the two technologies has enabled researchers to directly study individual bacterial cells, and revealed the complexity and heterogeneity present in both laboratory cultures and in environmental samples. Results are discussed with respect to viability analysis, stress induced changes, specific cell detection and cell sorting.",
keywords = "Cell sorting, Environmental bacteria, Specific identification, Viability assessment",
author = "Jonathan Porter and Daniel Deere and Roger Pickup and Clive Edwards",
year = "1996",
month = feb,
day = "1",
doi = "10.1002/(SICI)1097-0320(19960201)23:2<91::AID-CYTO1>3.0.CO;2-O",
language = "English",
volume = "23",
pages = "91--96",
journal = "Cytometry",
issn = "0196-4763",
publisher = "John Wiley & Sons Inc.",
number = "2",

}

RIS

TY - JOUR

T1 - Fluorescent probes and flow cytometry

T2 - New insights into environmental bacteriology

AU - Porter, Jonathan

AU - Deere, Daniel

AU - Pickup, Roger

AU - Edwards, Clive

PY - 1996/2/1

Y1 - 1996/2/1

N2 - Recent trends in flow cytometry have established new techniques in bacteriology. Advances in fluorescent dye technology complement these improvements, offering probes for a variety of cellular functions. Bacterial ecology requires the application of new techniques to help answer questions unanswerable by traditional methods alone. Here we review some aspects of how coupling the two technologies has enabled researchers to directly study individual bacterial cells, and revealed the complexity and heterogeneity present in both laboratory cultures and in environmental samples. Results are discussed with respect to viability analysis, stress induced changes, specific cell detection and cell sorting.

AB - Recent trends in flow cytometry have established new techniques in bacteriology. Advances in fluorescent dye technology complement these improvements, offering probes for a variety of cellular functions. Bacterial ecology requires the application of new techniques to help answer questions unanswerable by traditional methods alone. Here we review some aspects of how coupling the two technologies has enabled researchers to directly study individual bacterial cells, and revealed the complexity and heterogeneity present in both laboratory cultures and in environmental samples. Results are discussed with respect to viability analysis, stress induced changes, specific cell detection and cell sorting.

KW - Cell sorting

KW - Environmental bacteria

KW - Specific identification

KW - Viability assessment

U2 - 10.1002/(SICI)1097-0320(19960201)23:2<91::AID-CYTO1>3.0.CO;2-O

DO - 10.1002/(SICI)1097-0320(19960201)23:2<91::AID-CYTO1>3.0.CO;2-O

M3 - Review article

C2 - 8742166

AN - SCOPUS:0030064429

VL - 23

SP - 91

EP - 96

JO - Cytometry

JF - Cytometry

SN - 0196-4763

IS - 2

ER -