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Free flow isotachophoresis in an injection moulded miniaturised separation chamber with integrated electrodes

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Free flow isotachophoresis in an injection moulded miniaturised separation chamber with integrated electrodes. / Stone, Victoria N.; Baldock, Sara J.; Croasdell, Laura A. et al.
In: Journal of Chromatography A, Vol. 1155, No. 2, 2007, p. 199-205.

Research output: Contribution to Journal/MagazineJournal articlepeer-review

Harvard

Stone, VN, Baldock, SJ, Croasdell, LA, Dillon, LA, Fielden, PR, Goddard, NJ, Thomas, CLP & Brown, BJT 2007, 'Free flow isotachophoresis in an injection moulded miniaturised separation chamber with integrated electrodes', Journal of Chromatography A, vol. 1155, no. 2, pp. 199-205. https://doi.org/10.1016/j.chroma.2006.12.031

APA

Stone, V. N., Baldock, S. J., Croasdell, L. A., Dillon, L. A., Fielden, P. R., Goddard, N. J., Thomas, C. L. P., & Brown, B. J. T. (2007). Free flow isotachophoresis in an injection moulded miniaturised separation chamber with integrated electrodes. Journal of Chromatography A, 1155(2), 199-205. https://doi.org/10.1016/j.chroma.2006.12.031

Vancouver

Stone VN, Baldock SJ, Croasdell LA, Dillon LA, Fielden PR, Goddard NJ et al. Free flow isotachophoresis in an injection moulded miniaturised separation chamber with integrated electrodes. Journal of Chromatography A. 2007;1155(2):199-205. doi: 10.1016/j.chroma.2006.12.031

Author

Stone, Victoria N. ; Baldock, Sara J. ; Croasdell, Laura A. et al. / Free flow isotachophoresis in an injection moulded miniaturised separation chamber with integrated electrodes. In: Journal of Chromatography A. 2007 ; Vol. 1155, No. 2. pp. 199-205.

Bibtex

@article{0872d0287ad345b4b53a7b48fc32bc33,
title = "Free flow isotachophoresis in an injection moulded miniaturised separation chamber with integrated electrodes",
abstract = "An injection moulded free flow isotachophoresis (FFITP) microdevice with integrated carbon fibre loaded electrodes with a separation chamber of 36.4 mm wide, 28.7 mm long and 100 μm deep is presented. The microdevice was completely fabricated by injection moulding in carbon fibre loaded polystyrene for the electrodes and crystal polystyrene for the remainder of the chip and was bonded together using ultrasonic welding. Two injection moulded electrode designs were compared, one with the electrode surface level with the separation chamber and one with a recessed electrode. Separations of two anionic dyes, 0.2 mM each of amaranth and acid green and separations of 0.2 mM each of amaranth, bromophenol blue and glutamate were performed on the microdevice. Flow rates of 1.25 ml min−1 for the leading and terminating electrolytes were used and a flow rate of 0.63 ml min−1 for the sample. Electric fields of up to 370 V cm−1 were applied across the separation chamber. Joule heating was not found to be significant although out-gassing was observed at drive currents greater than 3 mA.",
keywords = "Free flow, Isotachophoresis, Electrophoresis, Miniaturisation, Polymer microfabrication",
author = "Stone, {Victoria N.} and Baldock, {Sara J.} and Croasdell, {Laura A.} and Dillon, {Leonard A.} and Fielden, {Peter R.} and Goddard, {Nick J.} and Thomas, {C. L. Paul} and Brown, {Bernard J. Treves}",
year = "2007",
doi = "10.1016/j.chroma.2006.12.031",
language = "English",
volume = "1155",
pages = "199--205",
journal = "Journal of Chromatography A",
issn = "0021-9673",
publisher = "Elsevier",
number = "2",

}

RIS

TY - JOUR

T1 - Free flow isotachophoresis in an injection moulded miniaturised separation chamber with integrated electrodes

AU - Stone, Victoria N.

AU - Baldock, Sara J.

AU - Croasdell, Laura A.

AU - Dillon, Leonard A.

AU - Fielden, Peter R.

AU - Goddard, Nick J.

AU - Thomas, C. L. Paul

AU - Brown, Bernard J. Treves

PY - 2007

Y1 - 2007

N2 - An injection moulded free flow isotachophoresis (FFITP) microdevice with integrated carbon fibre loaded electrodes with a separation chamber of 36.4 mm wide, 28.7 mm long and 100 μm deep is presented. The microdevice was completely fabricated by injection moulding in carbon fibre loaded polystyrene for the electrodes and crystal polystyrene for the remainder of the chip and was bonded together using ultrasonic welding. Two injection moulded electrode designs were compared, one with the electrode surface level with the separation chamber and one with a recessed electrode. Separations of two anionic dyes, 0.2 mM each of amaranth and acid green and separations of 0.2 mM each of amaranth, bromophenol blue and glutamate were performed on the microdevice. Flow rates of 1.25 ml min−1 for the leading and terminating electrolytes were used and a flow rate of 0.63 ml min−1 for the sample. Electric fields of up to 370 V cm−1 were applied across the separation chamber. Joule heating was not found to be significant although out-gassing was observed at drive currents greater than 3 mA.

AB - An injection moulded free flow isotachophoresis (FFITP) microdevice with integrated carbon fibre loaded electrodes with a separation chamber of 36.4 mm wide, 28.7 mm long and 100 μm deep is presented. The microdevice was completely fabricated by injection moulding in carbon fibre loaded polystyrene for the electrodes and crystal polystyrene for the remainder of the chip and was bonded together using ultrasonic welding. Two injection moulded electrode designs were compared, one with the electrode surface level with the separation chamber and one with a recessed electrode. Separations of two anionic dyes, 0.2 mM each of amaranth and acid green and separations of 0.2 mM each of amaranth, bromophenol blue and glutamate were performed on the microdevice. Flow rates of 1.25 ml min−1 for the leading and terminating electrolytes were used and a flow rate of 0.63 ml min−1 for the sample. Electric fields of up to 370 V cm−1 were applied across the separation chamber. Joule heating was not found to be significant although out-gassing was observed at drive currents greater than 3 mA.

KW - Free flow

KW - Isotachophoresis

KW - Electrophoresis

KW - Miniaturisation

KW - Polymer microfabrication

U2 - 10.1016/j.chroma.2006.12.031

DO - 10.1016/j.chroma.2006.12.031

M3 - Journal article

VL - 1155

SP - 199

EP - 205

JO - Journal of Chromatography A

JF - Journal of Chromatography A

SN - 0021-9673

IS - 2

ER -