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Functional similarities between phage Lambda Orf and Escherichia coli RecFOR in initiation of genetic exchange

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Functional similarities between phage Lambda Orf and Escherichia coli RecFOR in initiation of genetic exchange. / Maxwell, Karen; Reed, Patricia; Zhang, Rong-guang; Beasley, Steven; Walmsley, Adrian; Curtis, Fiona; Joachimiak, Andrej; Edwards, Aled; Sharples, Gary.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 102, No. 32, 09.08.2005, p. 11260-11265.

Research output: Contribution to Journal/MagazineJournal articlepeer-review

Harvard

Maxwell, K, Reed, P, Zhang, R, Beasley, S, Walmsley, A, Curtis, F, Joachimiak, A, Edwards, A & Sharples, G 2005, 'Functional similarities between phage Lambda Orf and Escherichia coli RecFOR in initiation of genetic exchange', Proceedings of the National Academy of Sciences of the United States of America, vol. 102, no. 32, pp. 11260-11265. https://doi.org/10.1073/pnas.0503399102

APA

Maxwell, K., Reed, P., Zhang, R., Beasley, S., Walmsley, A., Curtis, F., Joachimiak, A., Edwards, A., & Sharples, G. (2005). Functional similarities between phage Lambda Orf and Escherichia coli RecFOR in initiation of genetic exchange. Proceedings of the National Academy of Sciences of the United States of America, 102(32), 11260-11265. https://doi.org/10.1073/pnas.0503399102

Vancouver

Maxwell K, Reed P, Zhang R, Beasley S, Walmsley A, Curtis F et al. Functional similarities between phage Lambda Orf and Escherichia coli RecFOR in initiation of genetic exchange. Proceedings of the National Academy of Sciences of the United States of America. 2005 Aug 9;102(32):11260-11265. https://doi.org/10.1073/pnas.0503399102

Author

Maxwell, Karen ; Reed, Patricia ; Zhang, Rong-guang ; Beasley, Steven ; Walmsley, Adrian ; Curtis, Fiona ; Joachimiak, Andrej ; Edwards, Aled ; Sharples, Gary. / Functional similarities between phage Lambda Orf and Escherichia coli RecFOR in initiation of genetic exchange. In: Proceedings of the National Academy of Sciences of the United States of America. 2005 ; Vol. 102, No. 32. pp. 11260-11265.

Bibtex

@article{ecc93ce4e76e44b1b5b6ad379d4765e8,
title = "Functional similarities between phage Lambda Orf and Escherichia coli RecFOR in initiation of genetic exchange",
abstract = "Genetic recombination in bacteriophage relies on DNA end processing by Exo to expose 3'-tailed strands for annealing and exchange by protein. Phage encodes an additional recombinase, Orf, which participates in the early stages of recombination by supplying a function equivalent to the Escherichia coli RecFOR complex. These host enzymes assist loading of the RecA strand exchange protein onto ssDNA coated with ssDNA-binding protein. In this study, we purified the Orf protein, analyzed its biochemical properties, and determined its crystal structure at 2.5 {\AA}. The homodimeric Orf protein is arranged as a toroid with a shallow U-shaped cleft, lined with basic residues, running perpendicular to the central cavity. Orf binds DNA, favoring single-stranded over duplex and with no obvious preference for gapped, 3'-tailed, or 5'-tailed substrates. An interaction between Orf and ssDNA-binding protein was indicated by far Western analysis. The functional similarities between Orf and RecFOR are discussed in relation to the early steps of recombinational exchange and the interplay between phage and bacterial recombinases.",
keywords = "bacteriophage , DNA repair , genetic recombination, NinB",
author = "Karen Maxwell and Patricia Reed and Rong-guang Zhang and Steven Beasley and Adrian Walmsley and Fiona Curtis and Andrej Joachimiak and Aled Edwards and Gary Sharples",
year = "2005",
month = aug,
day = "9",
doi = "10.1073/pnas.0503399102",
language = "English",
volume = "102",
pages = "11260--11265",
journal = "Proceedings of the National Academy of Sciences of the United States of America",
issn = "0027-8424",
publisher = "National Academy of Sciences",
number = "32",

}

RIS

TY - JOUR

T1 - Functional similarities between phage Lambda Orf and Escherichia coli RecFOR in initiation of genetic exchange

AU - Maxwell, Karen

AU - Reed, Patricia

AU - Zhang, Rong-guang

AU - Beasley, Steven

AU - Walmsley, Adrian

AU - Curtis, Fiona

AU - Joachimiak, Andrej

AU - Edwards, Aled

AU - Sharples, Gary

PY - 2005/8/9

Y1 - 2005/8/9

N2 - Genetic recombination in bacteriophage relies on DNA end processing by Exo to expose 3'-tailed strands for annealing and exchange by protein. Phage encodes an additional recombinase, Orf, which participates in the early stages of recombination by supplying a function equivalent to the Escherichia coli RecFOR complex. These host enzymes assist loading of the RecA strand exchange protein onto ssDNA coated with ssDNA-binding protein. In this study, we purified the Orf protein, analyzed its biochemical properties, and determined its crystal structure at 2.5 Å. The homodimeric Orf protein is arranged as a toroid with a shallow U-shaped cleft, lined with basic residues, running perpendicular to the central cavity. Orf binds DNA, favoring single-stranded over duplex and with no obvious preference for gapped, 3'-tailed, or 5'-tailed substrates. An interaction between Orf and ssDNA-binding protein was indicated by far Western analysis. The functional similarities between Orf and RecFOR are discussed in relation to the early steps of recombinational exchange and the interplay between phage and bacterial recombinases.

AB - Genetic recombination in bacteriophage relies on DNA end processing by Exo to expose 3'-tailed strands for annealing and exchange by protein. Phage encodes an additional recombinase, Orf, which participates in the early stages of recombination by supplying a function equivalent to the Escherichia coli RecFOR complex. These host enzymes assist loading of the RecA strand exchange protein onto ssDNA coated with ssDNA-binding protein. In this study, we purified the Orf protein, analyzed its biochemical properties, and determined its crystal structure at 2.5 Å. The homodimeric Orf protein is arranged as a toroid with a shallow U-shaped cleft, lined with basic residues, running perpendicular to the central cavity. Orf binds DNA, favoring single-stranded over duplex and with no obvious preference for gapped, 3'-tailed, or 5'-tailed substrates. An interaction between Orf and ssDNA-binding protein was indicated by far Western analysis. The functional similarities between Orf and RecFOR are discussed in relation to the early steps of recombinational exchange and the interplay between phage and bacterial recombinases.

KW - bacteriophage

KW - DNA repair

KW - genetic recombination

KW - NinB

U2 - 10.1073/pnas.0503399102

DO - 10.1073/pnas.0503399102

M3 - Journal article

VL - 102

SP - 11260

EP - 11265

JO - Proceedings of the National Academy of Sciences of the United States of America

JF - Proceedings of the National Academy of Sciences of the United States of America

SN - 0027-8424

IS - 32

ER -