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Galantamine inhibits β-amyloid aggregation and cytotoxicity

Research output: Contribution to Journal/Magazine › Journal article › peer-review

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Galantamine inhibits β-amyloid aggregation and cytotoxicity. / Matharu, Balpreet; Gibson, Gillian; Parsons, Richard et al.
In: Journal of the Neurological Sciences, Vol. 280, No. 1-2, 15.05.2009, p. 49-58.

Research output: Contribution to Journal/Magazine › Journal article › peer-review

Harvard

Matharu, B, Gibson, G, Parsons, R, Huckerby, TN, Moore, SA, Cooper, LJ, Millichamp, R, Allsop, D & Austen, B 2009, 'Galantamine inhibits β-amyloid aggregation and cytotoxicity', Journal of the Neurological Sciences, vol. 280, no. 1-2, pp. 49-58. https://doi.org/10.1016/j.jns.2009.01.024

APA

Matharu, B., Gibson, G., Parsons, R., Huckerby, T. N., Moore, S. A., Cooper, L. J., Millichamp, R., Allsop, D., & Austen, B. (2009). Galantamine inhibits β-amyloid aggregation and cytotoxicity. Journal of the Neurological Sciences, 280(1-2), 49-58. https://doi.org/10.1016/j.jns.2009.01.024

Vancouver

Matharu B, Gibson G, Parsons R, Huckerby TN, Moore SA, Cooper LJ et al. Galantamine inhibits β-amyloid aggregation and cytotoxicity. Journal of the Neurological Sciences. 2009 May 15;280(1-2):49-58. doi: 10.1016/j.jns.2009.01.024

Author

Matharu, Balpreet ; Gibson, Gillian ; Parsons, Richard et al. / Galantamine inhibits β-amyloid aggregation and cytotoxicity. In: Journal of the Neurological Sciences. 2009 ; Vol. 280, No. 1-2. pp. 49-58.

Bibtex

@article{9c6fb9be34e94857a37554999d9dc823,
title = "Galantamine inhibits β-amyloid aggregation and cytotoxicity",
abstract = "The ability of galantamine (Reminyl) to inhibit the aggregation and toxicity of the beta-amyloid peptide (Abeta) was investigated. Galantamine showed concentration-dependent inhibition of aggregation of both Abeta 1-40 and Abeta 1-42, as determined by an ELISA method. Electron microscope studies of Abeta 1-40 incubated in the presence of galantamine revealed fibrils that were disordered and clumped in appearance. MTT and lactate dehydrogenase assays, employing SH-SY5Y human neuroblastoma cells, showed that galantamine reduced the cytotoxicity induced by Abeta 1-40. Galantamine also dramatically reduced Abeta 1-40-induced cellular apoptosis in these cells. There is some evidence that galantamine may not be acting purely as a symptomatic treatment. Disease-modifying effects of the drug could be due to an additional effect on Abeta aggregation and/or toxicity.",
keywords = "Amyloid beta-Peptides, Analysis of Variance, Apoptosis, Cell Line, Tumor, Cholinesterase Inhibitors, Dose-Response Relationship, Drug, Enzyme-Linked Immunosorbent Assay, Galantamine, Humans, L-Lactate Dehydrogenase, Microscopy, Electron, Models, Molecular, Neuroprotective Agents, Nuclear Magnetic Resonance, Biomolecular, Peptide Fragments",
author = "Balpreet Matharu and Gillian Gibson and Richard Parsons and Huckerby, {Thomas N} and Moore, {Susan A} and Cooper, {Leanne J} and Robert Millichamp and David Allsop and Brian Austen",
year = "2009",
month = may,
day = "15",
doi = "10.1016/j.jns.2009.01.024",
language = "English",
volume = "280",
pages = "49--58",
journal = "Journal of the Neurological Sciences",
publisher = "Elsevier",
number = "1-2",

}

RIS

TY - JOUR

T1 - Galantamine inhibits β-amyloid aggregation and cytotoxicity

AU - Matharu, Balpreet

AU - Gibson, Gillian

AU - Parsons, Richard

AU - Huckerby, Thomas N

AU - Moore, Susan A

AU - Cooper, Leanne J

AU - Millichamp, Robert

AU - Allsop, David

AU - Austen, Brian

PY - 2009/5/15

Y1 - 2009/5/15

N2 - The ability of galantamine (Reminyl) to inhibit the aggregation and toxicity of the beta-amyloid peptide (Abeta) was investigated. Galantamine showed concentration-dependent inhibition of aggregation of both Abeta 1-40 and Abeta 1-42, as determined by an ELISA method. Electron microscope studies of Abeta 1-40 incubated in the presence of galantamine revealed fibrils that were disordered and clumped in appearance. MTT and lactate dehydrogenase assays, employing SH-SY5Y human neuroblastoma cells, showed that galantamine reduced the cytotoxicity induced by Abeta 1-40. Galantamine also dramatically reduced Abeta 1-40-induced cellular apoptosis in these cells. There is some evidence that galantamine may not be acting purely as a symptomatic treatment. Disease-modifying effects of the drug could be due to an additional effect on Abeta aggregation and/or toxicity.

AB - The ability of galantamine (Reminyl) to inhibit the aggregation and toxicity of the beta-amyloid peptide (Abeta) was investigated. Galantamine showed concentration-dependent inhibition of aggregation of both Abeta 1-40 and Abeta 1-42, as determined by an ELISA method. Electron microscope studies of Abeta 1-40 incubated in the presence of galantamine revealed fibrils that were disordered and clumped in appearance. MTT and lactate dehydrogenase assays, employing SH-SY5Y human neuroblastoma cells, showed that galantamine reduced the cytotoxicity induced by Abeta 1-40. Galantamine also dramatically reduced Abeta 1-40-induced cellular apoptosis in these cells. There is some evidence that galantamine may not be acting purely as a symptomatic treatment. Disease-modifying effects of the drug could be due to an additional effect on Abeta aggregation and/or toxicity.

KW - Amyloid beta-Peptides

KW - Analysis of Variance

KW - Apoptosis

KW - Cell Line, Tumor

KW - Cholinesterase Inhibitors

KW - Dose-Response Relationship, Drug

KW - Enzyme-Linked Immunosorbent Assay

KW - Galantamine

KW - Humans

KW - L-Lactate Dehydrogenase

KW - Microscopy, Electron

KW - Models, Molecular

KW - Neuroprotective Agents

KW - Nuclear Magnetic Resonance, Biomolecular

KW - Peptide Fragments

U2 - 10.1016/j.jns.2009.01.024

DO - 10.1016/j.jns.2009.01.024

M3 - Journal article

C2 - 19249060

VL - 280

SP - 49

EP - 58

JO - Journal of the Neurological Sciences

JF - Journal of the Neurological Sciences

IS - 1-2

ER -