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Gametophytic and sporophytic expression of an anther-specific Arabidopsis thaliana gene

Research output: Contribution to Journal/Magazine › Journal article › peer-review

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Gametophytic and sporophytic expression of an anther-specific Arabidopsis thaliana gene. / Roberts, Michael; Foster, Gary D.; Blundell, Robert P. et al.
In: The Plant Journal, Vol. 3, No. 1, 01.1993, p. 111-120.

Research output: Contribution to Journal/Magazine › Journal article › peer-review

Harvard

Roberts, M, Foster, GD, Blundell, RP, Robinson, SW, Kumar, A, Draper, J & Scott, R 1993, 'Gametophytic and sporophytic expression of an anther-specific Arabidopsis thaliana gene', The Plant Journal, vol. 3, no. 1, pp. 111-120. https://doi.org/10.1046/j.1365-313X.1993.t01-5-00999.x

APA

Roberts, M., Foster, G. D., Blundell, R. P., Robinson, S. W., Kumar, A., Draper, J., & Scott, R. (1993). Gametophytic and sporophytic expression of an anther-specific Arabidopsis thaliana gene. The Plant Journal, 3(1), 111-120. https://doi.org/10.1046/j.1365-313X.1993.t01-5-00999.x

Vancouver

Roberts M, Foster GD, Blundell RP, Robinson SW, Kumar A, Draper J et al. Gametophytic and sporophytic expression of an anther-specific Arabidopsis thaliana gene. The Plant Journal. 1993 Jan;3(1):111-120. doi: 10.1046/j.1365-313X.1993.t01-5-00999.x

Author

Roberts, Michael ; Foster, Gary D. ; Blundell, Robert P. et al. / Gametophytic and sporophytic expression of an anther-specific Arabidopsis thaliana gene. In: The Plant Journal. 1993 ; Vol. 3, No. 1. pp. 111-120.

Bibtex

@article{7835ecd42ebb4230a6e78688b9b321d8,
title = "Gametophytic and sporophytic expression of an anther-specific Arabidopsis thaliana gene",
abstract = "Genomic and cDNA clones of the anther-specific APG gene from Arabidopsis thaliana and Brassica napus which encodes a novel proline-rich protein, were isolated and characterized. Southern blotting and Northern analysis of male fertile and cytoplasmic male sterile varieties of B. napus showed that the APG gene is present as a single copy in the Arabidopsis genome, and that the B. napus APG gene is a member of a small anther-specific gene family. Analysis of developmentally staged B. napus flower buds indicated that APG transcript is confined to the anther during the period of microspore development. Reporter gene fusions established that the APG promoter directs expression in a number of cell types in anthers of transformed plants. This expression is consistent with the temporal pattern of mRNA accumulation in B. napus buds and follows a complex developmental pattern. Most significantly, the promoter is active in both sporophytic and gametophytic cell types, with activity of the transgene in each cell type being delineated by various cytological markers.",
keywords = "gametophyte, sporophyte, anther, Gene expression",
author = "Michael Roberts and Foster, {Gary D.} and Blundell, {Robert P.} and Robinson, {Susan W.} and Amar Kumar and John Draper and Rod Scott",
year = "1993",
month = jan,
doi = "10.1046/j.1365-313X.1993.t01-5-00999.x",
language = "English",
volume = "3",
pages = "111--120",
journal = "The Plant Journal",
publisher = "Blackwell Publishing Ltd",
number = "1",

}

RIS

TY - JOUR

T1 - Gametophytic and sporophytic expression of an anther-specific Arabidopsis thaliana gene

AU - Roberts, Michael

AU - Foster, Gary D.

AU - Blundell, Robert P.

AU - Robinson, Susan W.

AU - Kumar, Amar

AU - Draper, John

AU - Scott, Rod

PY - 1993/1

Y1 - 1993/1

N2 - Genomic and cDNA clones of the anther-specific APG gene from Arabidopsis thaliana and Brassica napus which encodes a novel proline-rich protein, were isolated and characterized. Southern blotting and Northern analysis of male fertile and cytoplasmic male sterile varieties of B. napus showed that the APG gene is present as a single copy in the Arabidopsis genome, and that the B. napus APG gene is a member of a small anther-specific gene family. Analysis of developmentally staged B. napus flower buds indicated that APG transcript is confined to the anther during the period of microspore development. Reporter gene fusions established that the APG promoter directs expression in a number of cell types in anthers of transformed plants. This expression is consistent with the temporal pattern of mRNA accumulation in B. napus buds and follows a complex developmental pattern. Most significantly, the promoter is active in both sporophytic and gametophytic cell types, with activity of the transgene in each cell type being delineated by various cytological markers.

AB - Genomic and cDNA clones of the anther-specific APG gene from Arabidopsis thaliana and Brassica napus which encodes a novel proline-rich protein, were isolated and characterized. Southern blotting and Northern analysis of male fertile and cytoplasmic male sterile varieties of B. napus showed that the APG gene is present as a single copy in the Arabidopsis genome, and that the B. napus APG gene is a member of a small anther-specific gene family. Analysis of developmentally staged B. napus flower buds indicated that APG transcript is confined to the anther during the period of microspore development. Reporter gene fusions established that the APG promoter directs expression in a number of cell types in anthers of transformed plants. This expression is consistent with the temporal pattern of mRNA accumulation in B. napus buds and follows a complex developmental pattern. Most significantly, the promoter is active in both sporophytic and gametophytic cell types, with activity of the transgene in each cell type being delineated by various cytological markers.

KW - gametophyte

KW - sporophyte

KW - anther

KW - Gene expression

U2 - 10.1046/j.1365-313X.1993.t01-5-00999.x

DO - 10.1046/j.1365-313X.1993.t01-5-00999.x

M3 - Journal article

VL - 3

SP - 111

EP - 120

JO - The Plant Journal

JF - The Plant Journal

IS - 1

ER -