Final published version
Research output: Contribution to Journal/Magazine › Journal article › peer-review
Research output: Contribution to Journal/Magazine › Journal article › peer-review
}
TY - JOUR
T1 - Gel-free/label-free proteomic, photosynthetic, and biochemical analysis of cowpea ( Vigna unguiculata [L.] Walp.) resistance against Cowpea severe mosaic virus (CPSMV)
AU - Varela, Anna Lidia N.
AU - Komatsu, Setsuko
AU - Wang, Xin
AU - Silva, Rodolpho G.G.
AU - Souza, Pedro Filho N.
AU - Moreira Lobo, Ana Karla
AU - Vasconcelos, Ilka M.
AU - Silveira, Joaquim Albenisio Gomes
AU - Oliveira, Jose T.A.
PY - 2017/6/30
Y1 - 2017/6/30
N2 - Cowpea severe mosaic virus (CPSMV) causes significant losses in cowpea (Vigna unguiculata) production. In this present study biochemical, physiological, and proteomic analysis were done to identify pathways and defense proteins that are altered during the incompatible interaction between the cowpea genotype BRS-Marataoã and CPSMV. The leaf protein extracts from mock- (MI) and CPSMV-inoculated plantlets (V) were evaluated at 2 and 6 days post-inoculation (DPI). Data support the assumptions that increases in biochemical (high hydrogen peroxide, antioxidant enzymes, and secondary compounds) and physiological responses (high photosynthesis index and chlorophyll content), confirmed by label-free comparative proteomic approach, in which quantitative changes in proteasome proteins, proteins related to photosynthesis, redox homeostasis, regulation factors/RNA processing proteins were observed may be implicated in the resistance of BRS-Marataoã to CPSMV. This pioneering study provides information for the selection of specific pathways and proteins, altered in this incompatible relationship, which could be chosen as targets for detailed studies to advance our understanding of the molecular, physiological, and biochemistry basis of the resistance mechanism of cowpea and design approachs to engineer plants that are more productive.
AB - Cowpea severe mosaic virus (CPSMV) causes significant losses in cowpea (Vigna unguiculata) production. In this present study biochemical, physiological, and proteomic analysis were done to identify pathways and defense proteins that are altered during the incompatible interaction between the cowpea genotype BRS-Marataoã and CPSMV. The leaf protein extracts from mock- (MI) and CPSMV-inoculated plantlets (V) were evaluated at 2 and 6 days post-inoculation (DPI). Data support the assumptions that increases in biochemical (high hydrogen peroxide, antioxidant enzymes, and secondary compounds) and physiological responses (high photosynthesis index and chlorophyll content), confirmed by label-free comparative proteomic approach, in which quantitative changes in proteasome proteins, proteins related to photosynthesis, redox homeostasis, regulation factors/RNA processing proteins were observed may be implicated in the resistance of BRS-Marataoã to CPSMV. This pioneering study provides information for the selection of specific pathways and proteins, altered in this incompatible relationship, which could be chosen as targets for detailed studies to advance our understanding of the molecular, physiological, and biochemistry basis of the resistance mechanism of cowpea and design approachs to engineer plants that are more productive.
KW - Cowpea
KW - CPSMV
KW - incompatible interaction
KW - Biochemistry
KW - Physiology
KW - Gel free/label-free proteomic
U2 - 10.1016/j.jprot.2017.05.003
DO - 10.1016/j.jprot.2017.05.003
M3 - Journal article
VL - 163
SP - 76
EP - 91
JO - Journal of Proteomics
JF - Journal of Proteomics
SN - 1874-3919
ER -