Research output: Contribution to Journal/Magazine › Journal article › peer-review
Research output: Contribution to Journal/Magazine › Journal article › peer-review
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TY - JOUR
T1 - Genes required for osmoregulation and apical secretion in Caenorhabditis elegans
AU - Liégeois, Samuel
AU - Benedetto, Alexandre
AU - Michaux, Grégoire
AU - Belliard, Guillaume
AU - Labouesse, Michel
PY - 2007/2/1
Y1 - 2007/2/1
N2 - Few studies have investigated whether or not there is an interdependence between osmoregulation and vesicular trafficking. We previously showed that in Caenorhabditis elegans che-14 mutations affect osmoregulation, cuticle secretion, and sensory organ development. We report the identification of seven lethal mutations displaying che-14-like phenotypes, which define four new genes, rdy-1-rdy-4 (rod-like larval lethality and dye-filling defective). rdy-1, rdy-2, and rdy-4 mutations affect excretory canal function and cuticle formation. Moreover, rdy-1 and rdy-2 mutations reduce the amount of matrix material normally secreted by sheath cells in the amphid channel. In contrast, rdy-3 mutants have short cystic excretory canals, suggesting that it acts in a different process. rdy-1 encodes the vacuolar H+-ATPase a-subunit VHA-5, whereas rdy-2 encodes a new tetraspan protein. We suggest that RDY-1/VHA-5 acts upstream of RDY-2 and CHE-14 in some tissues, since it is required for their delivery to the epidermal, but not the amphid sheath, apical plasma membrane. Hence, the RDY-1/VHA-5 trafficking function appears essential in some cells and its proton pump function essential in others. Finally, we show that RDY-1/VHA-5 distribution changes prior to molting in parallel with that of actin microfilaments and propose a model for molting whereby actin provides a spatial cue for secretion.
AB - Few studies have investigated whether or not there is an interdependence between osmoregulation and vesicular trafficking. We previously showed that in Caenorhabditis elegans che-14 mutations affect osmoregulation, cuticle secretion, and sensory organ development. We report the identification of seven lethal mutations displaying che-14-like phenotypes, which define four new genes, rdy-1-rdy-4 (rod-like larval lethality and dye-filling defective). rdy-1, rdy-2, and rdy-4 mutations affect excretory canal function and cuticle formation. Moreover, rdy-1 and rdy-2 mutations reduce the amount of matrix material normally secreted by sheath cells in the amphid channel. In contrast, rdy-3 mutants have short cystic excretory canals, suggesting that it acts in a different process. rdy-1 encodes the vacuolar H+-ATPase a-subunit VHA-5, whereas rdy-2 encodes a new tetraspan protein. We suggest that RDY-1/VHA-5 acts upstream of RDY-2 and CHE-14 in some tissues, since it is required for their delivery to the epidermal, but not the amphid sheath, apical plasma membrane. Hence, the RDY-1/VHA-5 trafficking function appears essential in some cells and its proton pump function essential in others. Finally, we show that RDY-1/VHA-5 distribution changes prior to molting in parallel with that of actin microfilaments and propose a model for molting whereby actin provides a spatial cue for secretion.
KW - Actin Cytoskeleton
KW - Actins
KW - Amino Acid Sequence
KW - Animals
KW - Base Sequence
KW - Caenorhabditis elegans
KW - Caenorhabditis elegans Proteins
KW - Epidermis
KW - Genes, Helminth
KW - Genetic Testing
KW - Membrane Proteins
KW - Molecular Sequence Data
KW - Molting
KW - Mosaicism
KW - Mutagenesis
KW - Mutation
KW - Phenotype
KW - Vacuolar Proton-Translocating ATPases
KW - Water-Electrolyte Balance
KW - Journal Article
KW - Research Support, Non-U.S. Gov't
U2 - 10.1534/genetics.106.066035
DO - 10.1534/genetics.106.066035
M3 - Journal article
C2 - 17179093
VL - 175
SP - 709
EP - 724
JO - Genetics
JF - Genetics
SN - 0016-6731
IS - 2
ER -