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Genetic content of wild-type human cytomegalovirus

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Genetic content of wild-type human cytomegalovirus. / Dolan, Aidan; Cunningham, Charles; Hector, Ralph D. et al.
In: Journal of General Virology, Vol. 85, No. 5, 05.2004, p. 1301-1312.

Research output: Contribution to Journal/Magazine › Journal article › peer-review

Harvard

Dolan, A, Cunningham, C, Hector, RD, Hassan-Walker, AF, Lee, L, Addison, C, Dargan, DJ, McGeoch, DJ, Gatherer, D, Emery, VC, Griffiths, PD, Sinzger, C, McSharry, BP, Wilkinson, GWG & Davison, AJ 2004, 'Genetic content of wild-type human cytomegalovirus', Journal of General Virology, vol. 85, no. 5, pp. 1301-1312. https://doi.org/10.1099/vir.0.79888-0

APA

Dolan, A., Cunningham, C., Hector, R. D., Hassan-Walker, A. F., Lee, L., Addison, C., Dargan, D. J., McGeoch, D. J., Gatherer, D., Emery, V. C., Griffiths, P. D., Sinzger, C., McSharry, B. P., Wilkinson, G. W. G., & Davison, A. J. (2004). Genetic content of wild-type human cytomegalovirus. Journal of General Virology, 85(5), 1301-1312. https://doi.org/10.1099/vir.0.79888-0

Vancouver

Dolan A, Cunningham C, Hector RD, Hassan-Walker AF, Lee L, Addison C et al. Genetic content of wild-type human cytomegalovirus. Journal of General Virology. 2004 May;85(5):1301-1312. doi: 10.1099/vir.0.79888-0

Author

Dolan, Aidan ; Cunningham, Charles ; Hector, Ralph D. et al. / Genetic content of wild-type human cytomegalovirus. In: Journal of General Virology. 2004 ; Vol. 85, No. 5. pp. 1301-1312.

Bibtex

@article{87657aa6dd3b40d08ed090fe8d648c13,
title = "Genetic content of wild-type human cytomegalovirus",
abstract = "The genetic content of wild-type human cytomegalovirus was investigated by sequencing the 235 645 bp genome of a low passage strain (Merlin). Substantial regions of the genome (genes RL1-UL11, UL105-UL112 and UL120-UL150) were also sequenced in several other strains, including two that had not been passaged in cell culture. Comparative analyses, which employed the published genome sequence of a high passage strain (AD169), indicated that Merlin accurately reflects the wild-type complement of 165 genes, containing no obvious mutations other than a single nucleotide substitution that truncates gene UL128. A sizeable subset of genes exhibits unusually high variation between strains, and comprises many, but not all, of those that encode proteins known or predicted to be secreted or membrane-associated. In contrast to unpassaged strains, all of the passaged strains analysed have visibly disabling mutations in one or both of two groups of genes that may influence cell tropism. One comprises UL128, UL130 and UL131A, which putatively encode secreted proteins, and the other contains RL5A, RL13 and UL9, which are members of the RL11 glycoprotein gene family. The case in support of a lack of protein-coding potential in the region between UL105 and UL111 A was also strengthened.",
keywords = "OPEN READING FRAME, GENOME, SEQUENCE, STRAINS, PROTEIN, AD169, IDENTIFICATION, EXPRESSION, ENCODES, REGION",
author = "Aidan Dolan and Charles Cunningham and Hector, {Ralph D.} and Hassan-Walker, {Aycan F.} and Lydia Lee and Clare Addison and Dargan, {Derrick J.} and McGeoch, {Duncan J.} and Derek Gatherer and Emery, {Vincent C.} and Griffiths, {Paul D.} and Christian Sinzger and McSharry, {Brian P.} and Wilkinson, {Gavin W. G.} and Davison, {Andrew J.}",
year = "2004",
month = may,
doi = "10.1099/vir.0.79888-0",
language = "English",
volume = "85",
pages = "1301--1312",
journal = "Journal of General Virology",
issn = "0022-1317",
publisher = "Society for General Microbiology",
number = "5",

}

RIS

TY - JOUR

T1 - Genetic content of wild-type human cytomegalovirus

AU - Dolan, Aidan

AU - Cunningham, Charles

AU - Hector, Ralph D.

AU - Hassan-Walker, Aycan F.

AU - Lee, Lydia

AU - Addison, Clare

AU - Dargan, Derrick J.

AU - McGeoch, Duncan J.

AU - Gatherer, Derek

AU - Emery, Vincent C.

AU - Griffiths, Paul D.

AU - Sinzger, Christian

AU - McSharry, Brian P.

AU - Wilkinson, Gavin W. G.

AU - Davison, Andrew J.

PY - 2004/5

Y1 - 2004/5

N2 - The genetic content of wild-type human cytomegalovirus was investigated by sequencing the 235 645 bp genome of a low passage strain (Merlin). Substantial regions of the genome (genes RL1-UL11, UL105-UL112 and UL120-UL150) were also sequenced in several other strains, including two that had not been passaged in cell culture. Comparative analyses, which employed the published genome sequence of a high passage strain (AD169), indicated that Merlin accurately reflects the wild-type complement of 165 genes, containing no obvious mutations other than a single nucleotide substitution that truncates gene UL128. A sizeable subset of genes exhibits unusually high variation between strains, and comprises many, but not all, of those that encode proteins known or predicted to be secreted or membrane-associated. In contrast to unpassaged strains, all of the passaged strains analysed have visibly disabling mutations in one or both of two groups of genes that may influence cell tropism. One comprises UL128, UL130 and UL131A, which putatively encode secreted proteins, and the other contains RL5A, RL13 and UL9, which are members of the RL11 glycoprotein gene family. The case in support of a lack of protein-coding potential in the region between UL105 and UL111 A was also strengthened.

AB - The genetic content of wild-type human cytomegalovirus was investigated by sequencing the 235 645 bp genome of a low passage strain (Merlin). Substantial regions of the genome (genes RL1-UL11, UL105-UL112 and UL120-UL150) were also sequenced in several other strains, including two that had not been passaged in cell culture. Comparative analyses, which employed the published genome sequence of a high passage strain (AD169), indicated that Merlin accurately reflects the wild-type complement of 165 genes, containing no obvious mutations other than a single nucleotide substitution that truncates gene UL128. A sizeable subset of genes exhibits unusually high variation between strains, and comprises many, but not all, of those that encode proteins known or predicted to be secreted or membrane-associated. In contrast to unpassaged strains, all of the passaged strains analysed have visibly disabling mutations in one or both of two groups of genes that may influence cell tropism. One comprises UL128, UL130 and UL131A, which putatively encode secreted proteins, and the other contains RL5A, RL13 and UL9, which are members of the RL11 glycoprotein gene family. The case in support of a lack of protein-coding potential in the region between UL105 and UL111 A was also strengthened.

KW - OPEN READING FRAME

KW - GENOME

KW - SEQUENCE

KW - STRAINS

KW - PROTEIN

KW - AD169

KW - IDENTIFICATION

KW - EXPRESSION

KW - ENCODES

KW - REGION

U2 - 10.1099/vir.0.79888-0

DO - 10.1099/vir.0.79888-0

M3 - Journal article

VL - 85

SP - 1301

EP - 1312

JO - Journal of General Virology

JF - Journal of General Virology

SN - 0022-1317

IS - 5

ER -