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Research output: Contribution to Journal/Magazine › Journal article › peer-review
Research output: Contribution to Journal/Magazine › Journal article › peer-review
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TY - JOUR
T1 - Genetic structure and insecticide resistance characteristics of fall armyworm populations invading China
AU - Zhang, Lei
AU - Liu, Bo
AU - Zhang, Weigang
AU - Liu, Conghui
AU - Zhang, Dandan
AU - Zhao, Shengyuan
AU - Li, Zaiyuan
AU - Xu, Pengjun
AU - Wilson, Kenneth
AU - Withers, Amy
AU - Jones, Christopher M.
AU - Smith, Judith
AU - Chipabika, Gilson
AU - Kachigamba, Donald L.
AU - Nam, Kiwoong
AU - d'Alencon, Emmanuelle
AU - Liu, Bei
AU - Liang, Xinyue
AU - Jin, Minghui
AU - Wu, Chao
AU - Chakrabarty, Swapan
AU - Yang, Xianming
AU - Jiang, Yuying
AU - Liu, Jie
AU - Liu, Xiaolin
AU - Quan, Weipeng
AU - Wang, Guirong
AU - Fan, Wei
AU - Qian, Wanqiang
AU - Wu, Kongming
AU - Xiao, Yutao
PY - 2020/11/1
Y1 - 2020/11/1
N2 - The rapid wide-scale spread of fall armyworm (Spodoptera frugiperda) has caused serious crop losses34 globally. However, differences in the genetic background of subpopulations and the mechanisms of rapid adaptation behind the invasion are still not well understood. Here we report the assembly of a 390.38-M chromosome-level genome of fall armyworm using Pacific Bioscience (PacBio) and Hi-C sequencing technologies with scaffold N50 of 12.7 M consisting of 22260 annotated protein-coding genes. Genome-wide resequencing of 103 samples from 16 provinces in China revealed that the fall armyworm population comprises a complex inter-strain hybrid, mainly with the corn-strain genetic background and less of the rice-strain, which highlights the inaccuracy of strain identification using mitochondrial or Triosephosphate isomerase (Tpi) genes. Analysis of genes related to pesticide- and Bt-resistance showed that the risk of fall armyworm developing resistance to conventional pesticides is very high. Laboratory bioassay results showed that insects invading China carry resistance to organophosphate and pyrethroid pesticides, but are sensitive to genetically modified maize expressing Bacillus thuringiensis (Bt) toxins Cry1Ab in field experiments. Additionally, two mitochondrial fragments are inserted into the nuclear genome, and the insertion event occurred after the differentiation of the two strains. This study represents a valuable advance toward improving management strategies for fall armyworm.
AB - The rapid wide-scale spread of fall armyworm (Spodoptera frugiperda) has caused serious crop losses34 globally. However, differences in the genetic background of subpopulations and the mechanisms of rapid adaptation behind the invasion are still not well understood. Here we report the assembly of a 390.38-M chromosome-level genome of fall armyworm using Pacific Bioscience (PacBio) and Hi-C sequencing technologies with scaffold N50 of 12.7 M consisting of 22260 annotated protein-coding genes. Genome-wide resequencing of 103 samples from 16 provinces in China revealed that the fall armyworm population comprises a complex inter-strain hybrid, mainly with the corn-strain genetic background and less of the rice-strain, which highlights the inaccuracy of strain identification using mitochondrial or Triosephosphate isomerase (Tpi) genes. Analysis of genes related to pesticide- and Bt-resistance showed that the risk of fall armyworm developing resistance to conventional pesticides is very high. Laboratory bioassay results showed that insects invading China carry resistance to organophosphate and pyrethroid pesticides, but are sensitive to genetically modified maize expressing Bacillus thuringiensis (Bt) toxins Cry1Ab in field experiments. Additionally, two mitochondrial fragments are inserted into the nuclear genome, and the insertion event occurred after the differentiation of the two strains. This study represents a valuable advance toward improving management strategies for fall armyworm.
KW - Spodoptera frugiperda
KW - gene insertion
KW - subpopulations
KW - population structure
KW - resistance risk
U2 - 10.1111/1755-0998.13219
DO - 10.1111/1755-0998.13219
M3 - Journal article
VL - 20
SP - 1682
EP - 1696
JO - Molecular Ecology Resources
JF - Molecular Ecology Resources
SN - 1755-098X
IS - 6
ER -