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Genetic structure and insecticide resistance characteristics of fall armyworm populations invading China

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Genetic structure and insecticide resistance characteristics of fall armyworm populations invading China. / Zhang, Lei; Liu, Bo; Zhang, Weigang et al.

In: Molecular Ecology Resources, Vol. 20, No. 6, 01.11.2020, p. 1682-1696.

Research output: Contribution to Journal/MagazineJournal articlepeer-review

Harvard

Zhang, L, Liu, B, Zhang, W, Liu, C, Zhang, D, Zhao, S, Li, Z, Xu, P, Wilson, K, Withers, A, Jones, CM, Smith, J, Chipabika, G, Kachigamba, DL, Nam, K, d'Alencon, E, Liu, B, Liang, X, Jin, M, Wu, C, Chakrabarty, S, Yang, X, Jiang, Y, Liu, J, Liu, X, Quan, W, Wang, G, Fan, W, Qian, W, Wu, K & Xiao, Y 2020, 'Genetic structure and insecticide resistance characteristics of fall armyworm populations invading China', Molecular Ecology Resources, vol. 20, no. 6, pp. 1682-1696. https://doi.org/10.1111/1755-0998.13219

APA

Zhang, L., Liu, B., Zhang, W., Liu, C., Zhang, D., Zhao, S., Li, Z., Xu, P., Wilson, K., Withers, A., Jones, C. M., Smith, J., Chipabika, G., Kachigamba, D. L., Nam, K., d'Alencon, E., Liu, B., Liang, X., Jin, M., ... Xiao, Y. (2020). Genetic structure and insecticide resistance characteristics of fall armyworm populations invading China. Molecular Ecology Resources, 20(6), 1682-1696. https://doi.org/10.1111/1755-0998.13219

Vancouver

Zhang L, Liu B, Zhang W, Liu C, Zhang D, Zhao S et al. Genetic structure and insecticide resistance characteristics of fall armyworm populations invading China. Molecular Ecology Resources. 2020 Nov 1;20(6):1682-1696. Epub 2020 Jul 20. doi: 10.1111/1755-0998.13219

Author

Zhang, Lei ; Liu, Bo ; Zhang, Weigang et al. / Genetic structure and insecticide resistance characteristics of fall armyworm populations invading China. In: Molecular Ecology Resources. 2020 ; Vol. 20, No. 6. pp. 1682-1696.

Bibtex

@article{052df3d0bb34482ca2e2e80e501cde7f,
title = "Genetic structure and insecticide resistance characteristics of fall armyworm populations invading China",
abstract = "The rapid wide-scale spread of fall armyworm (Spodoptera frugiperda) has caused serious crop losses34 globally. However, differences in the genetic background of subpopulations and the mechanisms of rapid adaptation behind the invasion are still not well understood. Here we report the assembly of a 390.38-M chromosome-level genome of fall armyworm using Pacific Bioscience (PacBio) and Hi-C sequencing technologies with scaffold N50 of 12.7 M consisting of 22260 annotated protein-coding genes. Genome-wide resequencing of 103 samples from 16 provinces in China revealed that the fall armyworm population comprises a complex inter-strain hybrid, mainly with the corn-strain genetic background and less of the rice-strain, which highlights the inaccuracy of strain identification using mitochondrial or Triosephosphate isomerase (Tpi) genes. Analysis of genes related to pesticide- and Bt-resistance showed that the risk of fall armyworm developing resistance to conventional pesticides is very high. Laboratory bioassay results showed that insects invading China carry resistance to organophosphate and pyrethroid pesticides, but are sensitive to genetically modified maize expressing Bacillus thuringiensis (Bt) toxins Cry1Ab in field experiments. Additionally, two mitochondrial fragments are inserted into the nuclear genome, and the insertion event occurred after the differentiation of the two strains. This study represents a valuable advance toward improving management strategies for fall armyworm.",
keywords = "Spodoptera frugiperda, gene insertion, subpopulations, population structure, resistance risk",
author = "Lei Zhang and Bo Liu and Weigang Zhang and Conghui Liu and Dandan Zhang and Shengyuan Zhao and Zaiyuan Li and Pengjun Xu and Kenneth Wilson and Amy Withers and Jones, {Christopher M.} and Judith Smith and Gilson Chipabika and Kachigamba, {Donald L.} and Kiwoong Nam and Emmanuelle d'Alencon and Bei Liu and Xinyue Liang and Minghui Jin and Chao Wu and Swapan Chakrabarty and Xianming Yang and Yuying Jiang and Jie Liu and Xiaolin Liu and Weipeng Quan and Guirong Wang and Wei Fan and Wanqiang Qian and Kongming Wu and Yutao Xiao",
year = "2020",
month = nov,
day = "1",
doi = "10.1111/1755-0998.13219",
language = "English",
volume = "20",
pages = "1682--1696",
journal = "Molecular Ecology Resources",
issn = "1755-098X",
publisher = "Wiley-Blackwell",
number = "6",

}

RIS

TY - JOUR

T1 - Genetic structure and insecticide resistance characteristics of fall armyworm populations invading China

AU - Zhang, Lei

AU - Liu, Bo

AU - Zhang, Weigang

AU - Liu, Conghui

AU - Zhang, Dandan

AU - Zhao, Shengyuan

AU - Li, Zaiyuan

AU - Xu, Pengjun

AU - Wilson, Kenneth

AU - Withers, Amy

AU - Jones, Christopher M.

AU - Smith, Judith

AU - Chipabika, Gilson

AU - Kachigamba, Donald L.

AU - Nam, Kiwoong

AU - d'Alencon, Emmanuelle

AU - Liu, Bei

AU - Liang, Xinyue

AU - Jin, Minghui

AU - Wu, Chao

AU - Chakrabarty, Swapan

AU - Yang, Xianming

AU - Jiang, Yuying

AU - Liu, Jie

AU - Liu, Xiaolin

AU - Quan, Weipeng

AU - Wang, Guirong

AU - Fan, Wei

AU - Qian, Wanqiang

AU - Wu, Kongming

AU - Xiao, Yutao

PY - 2020/11/1

Y1 - 2020/11/1

N2 - The rapid wide-scale spread of fall armyworm (Spodoptera frugiperda) has caused serious crop losses34 globally. However, differences in the genetic background of subpopulations and the mechanisms of rapid adaptation behind the invasion are still not well understood. Here we report the assembly of a 390.38-M chromosome-level genome of fall armyworm using Pacific Bioscience (PacBio) and Hi-C sequencing technologies with scaffold N50 of 12.7 M consisting of 22260 annotated protein-coding genes. Genome-wide resequencing of 103 samples from 16 provinces in China revealed that the fall armyworm population comprises a complex inter-strain hybrid, mainly with the corn-strain genetic background and less of the rice-strain, which highlights the inaccuracy of strain identification using mitochondrial or Triosephosphate isomerase (Tpi) genes. Analysis of genes related to pesticide- and Bt-resistance showed that the risk of fall armyworm developing resistance to conventional pesticides is very high. Laboratory bioassay results showed that insects invading China carry resistance to organophosphate and pyrethroid pesticides, but are sensitive to genetically modified maize expressing Bacillus thuringiensis (Bt) toxins Cry1Ab in field experiments. Additionally, two mitochondrial fragments are inserted into the nuclear genome, and the insertion event occurred after the differentiation of the two strains. This study represents a valuable advance toward improving management strategies for fall armyworm.

AB - The rapid wide-scale spread of fall armyworm (Spodoptera frugiperda) has caused serious crop losses34 globally. However, differences in the genetic background of subpopulations and the mechanisms of rapid adaptation behind the invasion are still not well understood. Here we report the assembly of a 390.38-M chromosome-level genome of fall armyworm using Pacific Bioscience (PacBio) and Hi-C sequencing technologies with scaffold N50 of 12.7 M consisting of 22260 annotated protein-coding genes. Genome-wide resequencing of 103 samples from 16 provinces in China revealed that the fall armyworm population comprises a complex inter-strain hybrid, mainly with the corn-strain genetic background and less of the rice-strain, which highlights the inaccuracy of strain identification using mitochondrial or Triosephosphate isomerase (Tpi) genes. Analysis of genes related to pesticide- and Bt-resistance showed that the risk of fall armyworm developing resistance to conventional pesticides is very high. Laboratory bioassay results showed that insects invading China carry resistance to organophosphate and pyrethroid pesticides, but are sensitive to genetically modified maize expressing Bacillus thuringiensis (Bt) toxins Cry1Ab in field experiments. Additionally, two mitochondrial fragments are inserted into the nuclear genome, and the insertion event occurred after the differentiation of the two strains. This study represents a valuable advance toward improving management strategies for fall armyworm.

KW - Spodoptera frugiperda

KW - gene insertion

KW - subpopulations

KW - population structure

KW - resistance risk

U2 - 10.1111/1755-0998.13219

DO - 10.1111/1755-0998.13219

M3 - Journal article

VL - 20

SP - 1682

EP - 1696

JO - Molecular Ecology Resources

JF - Molecular Ecology Resources

SN - 1755-098X

IS - 6

ER -