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Genetic structure and regulation of isoprene synthase in Poplar (Populus spp.)

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Genetic structure and regulation of isoprene synthase in Poplar (Populus spp.). / Vickers, Claudia E.; Possell, Malcolm; Hewitt, C. N.; Mullineaux, Philip M.

In: Plant Molecular Biology, Vol. 73, No. 4-5, 07.2010, p. 547-558.

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Vickers, Claudia E. ; Possell, Malcolm ; Hewitt, C. N. ; Mullineaux, Philip M. / Genetic structure and regulation of isoprene synthase in Poplar (Populus spp.). In: Plant Molecular Biology. 2010 ; Vol. 73, No. 4-5. pp. 547-558.

Bibtex

@article{80e9ff82f8e24dc189ae31f06f081e43,
title = "Genetic structure and regulation of isoprene synthase in Poplar (Populus spp.)",
abstract = "Isoprene is a volatile 5-carbon hydrocarbon derived from the chloroplastic methylerythritol 2-C-methyl-d-erythritol 4-phosphate isoprenoid pathway. In plants, isoprene emission is controlled by the enzyme isoprene synthase; however, there is still relatively little known about the genetics and regulation of this enzyme. Isoprene synthase gene structure was analysed in three poplar species. It was found that genes encoding stromal isoprene synthase exist as a small gene family, the members of which encode virtually identical proteins and are differentially regulated. Accumulation of isoprene synthase protein is developmentally regulated, but does not differ between sun and shade leaves and does not increase when heat stress is applied. Our data suggest that, in mature leaves, isoprene emission rates are primarily determined by substrate (dimethylallyl diphosphate, DMADP) availability. In immature leaves, where isoprene synthase levels are variable, emission levels are also influenced by the amount of isoprene synthase protein. No thylakoid isoforms could be identified in Populus alba or in Salix babylonica. Together, these data show that control of isoprene emission at the genetic level is far more complicated than previously assumed.",
keywords = "Isoprene, Poplar, Isoprene synthase, Developmental regulation, Isoprenoid pathway, VOLATILE ORGANIC-COMPOUNDS, DIMETHYLALLYL DIPHOSPHATE, MESSENGER-RNA, QUERCUS-ROBUR, ESCHERICHIA-COLI, LEAVES, EMISSION, PLANTS, ASPEN, HYDROCARBONS",
author = "Vickers, {Claudia E.} and Malcolm Possell and Hewitt, {C. N.} and Mullineaux, {Philip M.}",
year = "2010",
month = jul,
doi = "10.1007/s11103-010-9642-3",
language = "English",
volume = "73",
pages = "547--558",
journal = "Plant Molecular Biology",
issn = "0167-4412",
publisher = "Springer Netherlands",
number = "4-5",

}

RIS

TY - JOUR

T1 - Genetic structure and regulation of isoprene synthase in Poplar (Populus spp.)

AU - Vickers, Claudia E.

AU - Possell, Malcolm

AU - Hewitt, C. N.

AU - Mullineaux, Philip M.

PY - 2010/7

Y1 - 2010/7

N2 - Isoprene is a volatile 5-carbon hydrocarbon derived from the chloroplastic methylerythritol 2-C-methyl-d-erythritol 4-phosphate isoprenoid pathway. In plants, isoprene emission is controlled by the enzyme isoprene synthase; however, there is still relatively little known about the genetics and regulation of this enzyme. Isoprene synthase gene structure was analysed in three poplar species. It was found that genes encoding stromal isoprene synthase exist as a small gene family, the members of which encode virtually identical proteins and are differentially regulated. Accumulation of isoprene synthase protein is developmentally regulated, but does not differ between sun and shade leaves and does not increase when heat stress is applied. Our data suggest that, in mature leaves, isoprene emission rates are primarily determined by substrate (dimethylallyl diphosphate, DMADP) availability. In immature leaves, where isoprene synthase levels are variable, emission levels are also influenced by the amount of isoprene synthase protein. No thylakoid isoforms could be identified in Populus alba or in Salix babylonica. Together, these data show that control of isoprene emission at the genetic level is far more complicated than previously assumed.

AB - Isoprene is a volatile 5-carbon hydrocarbon derived from the chloroplastic methylerythritol 2-C-methyl-d-erythritol 4-phosphate isoprenoid pathway. In plants, isoprene emission is controlled by the enzyme isoprene synthase; however, there is still relatively little known about the genetics and regulation of this enzyme. Isoprene synthase gene structure was analysed in three poplar species. It was found that genes encoding stromal isoprene synthase exist as a small gene family, the members of which encode virtually identical proteins and are differentially regulated. Accumulation of isoprene synthase protein is developmentally regulated, but does not differ between sun and shade leaves and does not increase when heat stress is applied. Our data suggest that, in mature leaves, isoprene emission rates are primarily determined by substrate (dimethylallyl diphosphate, DMADP) availability. In immature leaves, where isoprene synthase levels are variable, emission levels are also influenced by the amount of isoprene synthase protein. No thylakoid isoforms could be identified in Populus alba or in Salix babylonica. Together, these data show that control of isoprene emission at the genetic level is far more complicated than previously assumed.

KW - Isoprene

KW - Poplar

KW - Isoprene synthase

KW - Developmental regulation

KW - Isoprenoid pathway

KW - VOLATILE ORGANIC-COMPOUNDS

KW - DIMETHYLALLYL DIPHOSPHATE

KW - MESSENGER-RNA

KW - QUERCUS-ROBUR

KW - ESCHERICHIA-COLI

KW - LEAVES

KW - EMISSION

KW - PLANTS

KW - ASPEN

KW - HYDROCARBONS

U2 - 10.1007/s11103-010-9642-3

DO - 10.1007/s11103-010-9642-3

M3 - Journal article

VL - 73

SP - 547

EP - 558

JO - Plant Molecular Biology

JF - Plant Molecular Biology

SN - 0167-4412

IS - 4-5

ER -