Final published version
Research output: Contribution to Journal/Magazine › Conference article › peer-review
Research output: Contribution to Journal/Magazine › Conference article › peer-review
}
TY - JOUR
T1 - Glucose oxidation using oxygen resistant pyranose-2-oxidase for biofuel cell applications
AU - Sahin, Samet
AU - Wongnate, Thanyaporn
AU - Chaiyen, Pimchai
AU - Yu, Eileen H.
PY - 2014/10/20
Y1 - 2014/10/20
N2 - In this study, the effect of oxygen on glucose oxidation using Glucose Oxidase (GOx) and oxygen resistant Pyranose-2-Oxidase (P2O) has been studied. Enzyme solutions with ferrocene carboxylic acid (FcCOOH) as electron mediator were tested with glassy carbon electrode (GCE) under air and nitrogen saturated conditions in a three electrode electrochemical cell system. Electrochemical characterization of enzymes has been achieved in solution by using cyclic voltammetry (CV), linear sweep voltammetry (LSV) and chronoamperometry (CA). In the presence of glucose, CV and LSV results show increasing anodic peak current and decreasing cathodic peak current with increasing glucose concentrations, which reflects the ferrocene-mediated bioelectrocatalysis of glucose oxidation. The experiments with CA show enhanced stability with oxygen resistant P2O where GOx loses 30 % of its current density in the presence of oxygen after 3 hours. These results indicate that P2O, a promising enzyme with no oxygen reactivity and long stability, which can be used in enzymatic biofuel cell applications as an alternative to GOx.
AB - In this study, the effect of oxygen on glucose oxidation using Glucose Oxidase (GOx) and oxygen resistant Pyranose-2-Oxidase (P2O) has been studied. Enzyme solutions with ferrocene carboxylic acid (FcCOOH) as electron mediator were tested with glassy carbon electrode (GCE) under air and nitrogen saturated conditions in a three electrode electrochemical cell system. Electrochemical characterization of enzymes has been achieved in solution by using cyclic voltammetry (CV), linear sweep voltammetry (LSV) and chronoamperometry (CA). In the presence of glucose, CV and LSV results show increasing anodic peak current and decreasing cathodic peak current with increasing glucose concentrations, which reflects the ferrocene-mediated bioelectrocatalysis of glucose oxidation. The experiments with CA show enhanced stability with oxygen resistant P2O where GOx loses 30 % of its current density in the presence of oxygen after 3 hours. These results indicate that P2O, a promising enzyme with no oxygen reactivity and long stability, which can be used in enzymatic biofuel cell applications as an alternative to GOx.
U2 - 10.3303/CET1441062
DO - 10.3303/CET1441062
M3 - Conference article
AN - SCOPUS:84908618474
VL - 41
SP - 367
EP - 372
JO - Chemical Engineering Transactions
JF - Chemical Engineering Transactions
SN - 2283-9216
IS - Special Issue
T2 - 10th European Symposium on Electrochemical Engineering, ESEE 2014
Y2 - 28 September 2014 through 2 October 2014
ER -