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Glutathione modulates the level of free radicals produced in UVA irradiated cells.

Research output: Contribution to Journal/Magazine › Journal article › peer-review

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Glutathione modulates the level of free radicals produced in UVA irradiated cells. / Tobi, Simon E.; Paul, Nigel; McMillan, Trevor J.
In: Journal of Photochemistry and Photobiology B: Biology, Vol. 57, No. 2-3, 09.2001, p. 102-112.

Research output: Contribution to Journal/Magazine › Journal article › peer-review

Harvard

Tobi, SE, Paul, N & McMillan, TJ 2001, 'Glutathione modulates the level of free radicals produced in UVA irradiated cells.', Journal of Photochemistry and Photobiology B: Biology, vol. 57, no. 2-3, pp. 102-112. https://doi.org/10.1016/S1011-1344(00)00084-1

APA

Tobi, S. E., Paul, N., & McMillan, T. J. (2001). Glutathione modulates the level of free radicals produced in UVA irradiated cells. Journal of Photochemistry and Photobiology B: Biology, 57(2-3), 102-112. https://doi.org/10.1016/S1011-1344(00)00084-1

Vancouver

Tobi SE, Paul N, McMillan TJ. Glutathione modulates the level of free radicals produced in UVA irradiated cells. Journal of Photochemistry and Photobiology B: Biology. 2001 Sept;57(2-3):102-112. doi: 10.1016/S1011-1344(00)00084-1

Author

Tobi, Simon E. ; Paul, Nigel ; McMillan, Trevor J. / Glutathione modulates the level of free radicals produced in UVA irradiated cells. In: Journal of Photochemistry and Photobiology B: Biology. 2001 ; Vol. 57, No. 2-3. pp. 102-112.

Bibtex

@article{470b920bd2904feaa972dc1656954b28,
title = "Glutathione modulates the level of free radicals produced in UVA irradiated cells.",
abstract = "We have developed an assay to detect reactive oxygen species (ROS) generated by UVA radiation utilising chemical probes which become fluorescent upon oxidation. Using a human bladder carcinoma cell line (MGH-U1) and spontaneously immortalised keratinocytes (HaCaT), we have shown a UVA (narrow band 365±5 nm) dose-dependent increase in fluorescence by flow cytometry following loading of the cells with either dihydrorhodamine 123 (DHR) or 2′,7′-dichlorodihydrofluorescein diacetate (DCFH-DA). The UVA response of both DHR and DCFH was enhanced by elevation of intracellular levels of the photosensitiser protoporphyrin IX by incubation for 2.5 h with 5-aminolaevulinic acid. Depletion of the antioxidant glutathione (GSH) using the inhibitor -buthionine-sulphoximine (BSO), resulted in an increase in the UVA-induced fluorescence of DCF but not of rhodamine 123. Conversely, raising intracellular GSH levels with N-acetyl cysteine (NAC) had relatively little protective effect in terms of degree of induced fluorescence.",
keywords = "UVA, Reactive oxygen species, Glutathione, 2′, 7′-Dichlorodihydrofluorescein diacetate",
author = "Tobi, {Simon E.} and Nigel Paul and McMillan, {Trevor J.}",
year = "2001",
month = sep,
doi = "10.1016/S1011-1344(00)00084-1",
language = "English",
volume = "57",
pages = "102--112",
journal = "Journal of Photochemistry and Photobiology B: Biology",
issn = "1011-1344",
publisher = "Elsevier",
number = "2-3",

}

RIS

TY - JOUR

T1 - Glutathione modulates the level of free radicals produced in UVA irradiated cells.

AU - Tobi, Simon E.

AU - Paul, Nigel

AU - McMillan, Trevor J.

PY - 2001/9

Y1 - 2001/9

N2 - We have developed an assay to detect reactive oxygen species (ROS) generated by UVA radiation utilising chemical probes which become fluorescent upon oxidation. Using a human bladder carcinoma cell line (MGH-U1) and spontaneously immortalised keratinocytes (HaCaT), we have shown a UVA (narrow band 365±5 nm) dose-dependent increase in fluorescence by flow cytometry following loading of the cells with either dihydrorhodamine 123 (DHR) or 2′,7′-dichlorodihydrofluorescein diacetate (DCFH-DA). The UVA response of both DHR and DCFH was enhanced by elevation of intracellular levels of the photosensitiser protoporphyrin IX by incubation for 2.5 h with 5-aminolaevulinic acid. Depletion of the antioxidant glutathione (GSH) using the inhibitor -buthionine-sulphoximine (BSO), resulted in an increase in the UVA-induced fluorescence of DCF but not of rhodamine 123. Conversely, raising intracellular GSH levels with N-acetyl cysteine (NAC) had relatively little protective effect in terms of degree of induced fluorescence.

AB - We have developed an assay to detect reactive oxygen species (ROS) generated by UVA radiation utilising chemical probes which become fluorescent upon oxidation. Using a human bladder carcinoma cell line (MGH-U1) and spontaneously immortalised keratinocytes (HaCaT), we have shown a UVA (narrow band 365±5 nm) dose-dependent increase in fluorescence by flow cytometry following loading of the cells with either dihydrorhodamine 123 (DHR) or 2′,7′-dichlorodihydrofluorescein diacetate (DCFH-DA). The UVA response of both DHR and DCFH was enhanced by elevation of intracellular levels of the photosensitiser protoporphyrin IX by incubation for 2.5 h with 5-aminolaevulinic acid. Depletion of the antioxidant glutathione (GSH) using the inhibitor -buthionine-sulphoximine (BSO), resulted in an increase in the UVA-induced fluorescence of DCF but not of rhodamine 123. Conversely, raising intracellular GSH levels with N-acetyl cysteine (NAC) had relatively little protective effect in terms of degree of induced fluorescence.

KW - UVA

KW - Reactive oxygen species

KW - Glutathione

KW - 2′

KW - 7′-Dichlorodihydrofluorescein diacetate

U2 - 10.1016/S1011-1344(00)00084-1

DO - 10.1016/S1011-1344(00)00084-1

M3 - Journal article

VL - 57

SP - 102

EP - 112

JO - Journal of Photochemistry and Photobiology B: Biology

JF - Journal of Photochemistry and Photobiology B: Biology

SN - 1011-1344

IS - 2-3

ER -