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Go with the flow - Use of flow cytometry in environmental microbiology

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Go with the flow - Use of flow cytometry in environmental microbiology. / Porter, Jonathan; Deere, Daniel; Hardman, Melanie et al.
In: FEMS Microbiology Ecology, Vol. 24, No. 2, 01.10.1997, p. 93-101.

Research output: Contribution to Journal/Magazine › Review article › peer-review

Harvard

Porter, J, Deere, D, Hardman, M, Edwards, C & Pickup, R 1997, 'Go with the flow - Use of flow cytometry in environmental microbiology', FEMS Microbiology Ecology, vol. 24, no. 2, pp. 93-101. https://doi.org/10.1111/j.1574-6941.1997.tb00426.x

APA

Porter, J., Deere, D., Hardman, M., Edwards, C., & Pickup, R. (1997). Go with the flow - Use of flow cytometry in environmental microbiology. FEMS Microbiology Ecology, 24(2), 93-101. https://doi.org/10.1111/j.1574-6941.1997.tb00426.x

Vancouver

Porter J, Deere D, Hardman M, Edwards C, Pickup R. Go with the flow - Use of flow cytometry in environmental microbiology. FEMS Microbiology Ecology. 1997 Oct 1;24(2):93-101. doi: 10.1111/j.1574-6941.1997.tb00426.x

Author

Porter, Jonathan ; Deere, Daniel ; Hardman, Melanie et al. / Go with the flow - Use of flow cytometry in environmental microbiology. In: FEMS Microbiology Ecology. 1997 ; Vol. 24, No. 2. pp. 93-101.

Bibtex

@article{41dcf1ff267949d2afa7d486cec0978d,
title = "Go with the flow - Use of flow cytometry in environmental microbiology",
abstract = "Microbiological activity in the natural world is of key importance in the integrated functioning of ecosystems, yet we remain largely ignorant of the role and relevance of the vast majority of microorganisms. This ignorance is largely due to widely acknowledged, but unresolved problems in methodologies. Application of flow cytometry to such studies has already revolutionised our understanding of marine photosynthetic planktonic microorganisms, revealed new levels of complexity in the behaviour of bacterial populations and produced a reliable screening protocol for eukaryotic water-borne pathogens. Advances in fluorescent probe technology now offer realistic approaches for direct cell identification, viability assessment and responses to environmental changes using basic, single light-source flow cytometers. Here we review current applications of flow cytometry in environmental microbiology and present a case for the adoption of the technique as a necessary and routine research instrument.",
keywords = "Direct method, Flow cytometry, Specific detection, Viability assessment",
author = "Jonathan Porter and Daniel Deere and Melanie Hardman and Clive Edwards and Roger Pickup",
year = "1997",
month = oct,
day = "1",
doi = "10.1111/j.1574-6941.1997.tb00426.x",
language = "English",
volume = "24",
pages = "93--101",
journal = "FEMS Microbiology Ecology",
issn = "0168-6496",
publisher = "Wiley-Blackwell",
number = "2",

}

RIS

TY - JOUR

T1 - Go with the flow - Use of flow cytometry in environmental microbiology

AU - Porter, Jonathan

AU - Deere, Daniel

AU - Hardman, Melanie

AU - Edwards, Clive

AU - Pickup, Roger

PY - 1997/10/1

Y1 - 1997/10/1

N2 - Microbiological activity in the natural world is of key importance in the integrated functioning of ecosystems, yet we remain largely ignorant of the role and relevance of the vast majority of microorganisms. This ignorance is largely due to widely acknowledged, but unresolved problems in methodologies. Application of flow cytometry to such studies has already revolutionised our understanding of marine photosynthetic planktonic microorganisms, revealed new levels of complexity in the behaviour of bacterial populations and produced a reliable screening protocol for eukaryotic water-borne pathogens. Advances in fluorescent probe technology now offer realistic approaches for direct cell identification, viability assessment and responses to environmental changes using basic, single light-source flow cytometers. Here we review current applications of flow cytometry in environmental microbiology and present a case for the adoption of the technique as a necessary and routine research instrument.

AB - Microbiological activity in the natural world is of key importance in the integrated functioning of ecosystems, yet we remain largely ignorant of the role and relevance of the vast majority of microorganisms. This ignorance is largely due to widely acknowledged, but unresolved problems in methodologies. Application of flow cytometry to such studies has already revolutionised our understanding of marine photosynthetic planktonic microorganisms, revealed new levels of complexity in the behaviour of bacterial populations and produced a reliable screening protocol for eukaryotic water-borne pathogens. Advances in fluorescent probe technology now offer realistic approaches for direct cell identification, viability assessment and responses to environmental changes using basic, single light-source flow cytometers. Here we review current applications of flow cytometry in environmental microbiology and present a case for the adoption of the technique as a necessary and routine research instrument.

KW - Direct method

KW - Flow cytometry

KW - Specific detection

KW - Viability assessment

U2 - 10.1111/j.1574-6941.1997.tb00426.x

DO - 10.1111/j.1574-6941.1997.tb00426.x

M3 - Review article

AN - SCOPUS:0030725473

VL - 24

SP - 93

EP - 101

JO - FEMS Microbiology Ecology

JF - FEMS Microbiology Ecology

SN - 0168-6496

IS - 2

ER -