Research output: Contribution to Journal/Magazine › Journal article › peer-review
Research output: Contribution to Journal/Magazine › Journal article › peer-review
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TY - JOUR
T1 - Grasses of different C4 subtypes reveal leaf traits related to drought tolerance in their natural habitats
T2 - changes in structure, water potential, and amino acid content
AU - Carmo-Silva, Ana E.
AU - Francisco, Ana
AU - Powers, Stephen J.
AU - Keys, Alfred J.
AU - Ascensão, Lia
AU - Parry, Martin A. J.
AU - Arrabaça, Maria Celeste
PY - 2009/7/1
Y1 - 2009/7/1
N2 - Three grasses (Poaceae) of different C4 subtypes, Paspalum dilatatum (NADP-malic enzyme [ME]), Cynodon dactylon (NAD-ME) and Zoysia japonica (phosphoenolpyruvate carboxykinase), occur in natural habitats that differ in annual rainfall. Their leaf characteristics were studied to identify traits related to drought tolerance. Plants were grown in pots, and water deficit was gradually induced by withholding water. Leaves of Z. japonica had the greatest and P. dilatatum the lowest relative dry matter content. Transverse sections of leaves that developed during the water deficit showed little change compared to control leaves, consistent with low phenotypic plasticity. Anatomical features distinguished the three species, with xeromorphic characteristics most strongly represented in Z. japonica. The leaf relative water content (RWC) decreased with the soil water content similarly for the three grasses. However, at 80% RWC, the leaf water potential was - 3.1 MPa for Z. japonica and only - 1.3 MPa for P. dilatatum and C. dactylon. Soluble amino acids, especially proline, increased as RWC decreased in leaves of C. dactylon and Z. japonica. Phenylalanine, valine, leucine, and isoleucine increased more in Z. japonica than in the other two species. The results provide evidence that C. dactylon and, especially, Z. japonica have evolved leaf traits better suited to arid habitats.
AB - Three grasses (Poaceae) of different C4 subtypes, Paspalum dilatatum (NADP-malic enzyme [ME]), Cynodon dactylon (NAD-ME) and Zoysia japonica (phosphoenolpyruvate carboxykinase), occur in natural habitats that differ in annual rainfall. Their leaf characteristics were studied to identify traits related to drought tolerance. Plants were grown in pots, and water deficit was gradually induced by withholding water. Leaves of Z. japonica had the greatest and P. dilatatum the lowest relative dry matter content. Transverse sections of leaves that developed during the water deficit showed little change compared to control leaves, consistent with low phenotypic plasticity. Anatomical features distinguished the three species, with xeromorphic characteristics most strongly represented in Z. japonica. The leaf relative water content (RWC) decreased with the soil water content similarly for the three grasses. However, at 80% RWC, the leaf water potential was - 3.1 MPa for Z. japonica and only - 1.3 MPa for P. dilatatum and C. dactylon. Soluble amino acids, especially proline, increased as RWC decreased in leaves of C. dactylon and Z. japonica. Phenylalanine, valine, leucine, and isoleucine increased more in Z. japonica than in the other two species. The results provide evidence that C. dactylon and, especially, Z. japonica have evolved leaf traits better suited to arid habitats.
KW - C grasses
KW - Cynodon dactylon
KW - Drought stress
KW - Leaf anatomy
KW - Paspalum dilatatum
KW - Poaceae
KW - Water deficit
KW - Zoysia japonica
U2 - 10.3732/ajb.0800224
DO - 10.3732/ajb.0800224
M3 - Journal article
AN - SCOPUS:64949083328
VL - 96
SP - 1222
EP - 1235
JO - American Journal of Botany
JF - American Journal of Botany
SN - 0002-9122
IS - 7
ER -