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Growth factor mRNA and protein in preserved human amniotic membrane.

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Growth factor mRNA and protein in preserved human amniotic membrane. / Koizumi, Noriko; Inatomi, Tsutomo; Sotozono, Chie et al.
In: Current Eye Research, Vol. 20, No. 3, 2000, p. 173-177.

Research output: Contribution to Journal/MagazineJournal articlepeer-review

Harvard

Koizumi, N, Inatomi, T, Sotozono, C, Fullwood, NJ, Quantock, AJ & Kinoshita, S 2000, 'Growth factor mRNA and protein in preserved human amniotic membrane.', Current Eye Research, vol. 20, no. 3, pp. 173-177. https://doi.org/10.1076/0271-3683(200003)20:3;1-9;FT173

APA

Koizumi, N., Inatomi, T., Sotozono, C., Fullwood, N. J., Quantock, A. J., & Kinoshita, S. (2000). Growth factor mRNA and protein in preserved human amniotic membrane. Current Eye Research, 20(3), 173-177. https://doi.org/10.1076/0271-3683(200003)20:3;1-9;FT173

Vancouver

Koizumi N, Inatomi T, Sotozono C, Fullwood NJ, Quantock AJ, Kinoshita S. Growth factor mRNA and protein in preserved human amniotic membrane. Current Eye Research. 2000;20(3):173-177. doi: 10.1076/0271-3683(200003)20:3;1-9;FT173

Author

Koizumi, Noriko ; Inatomi, Tsutomo ; Sotozono, Chie et al. / Growth factor mRNA and protein in preserved human amniotic membrane. In: Current Eye Research. 2000 ; Vol. 20, No. 3. pp. 173-177.

Bibtex

@article{45e43b7303f14f20a742e8d6e0054fb0,
title = "Growth factor mRNA and protein in preserved human amniotic membrane.",
abstract = "Purpose. To investigate the expression of growth factor mRNA and the level of growth factor protein in preserved human amniotic membrane (AM). Methods. RT-PCR was used to examine the expression of mRNA for eight growth factors (EGF, TGF-a, KGF, HGF, bFGF, TGF-1, -2, -3) and two growth factor receptors (KGFR and HGFR) in human AM preserved at -80°C for one month. In addition, ELISAs were used to measure the protein concentrations of seven growth factors (EGF, TGF-a, KGF, HGF, bFGF, TGF-1, -2) in preserved human corneas and in AM both with and without amniotic epithelium. Results. RT-PCR revealed that human AM expresses mRNA for EGF, TGF-a, KGF, HGF, bFGF, TGF-1, -2, -3, KGFR and HGFR, while ELISAs showed that it contains EGF, TGF-a, KGF, HGF, bFGF, TGF-1, -2. AM without amniotic epithelium also contains all seven growth factors examined, however, in this tissue the protein levels of EGF, KGF, HGF and bFGF were found to be significantly lower than in native AM. Conclusions. Preserved human AM expresses mRNAs for a number of growth factors and contains several growth factor proteins that might benefit epithelialization after AM transplantation. High levels of EGF, KGF, HGF and bFGF in AM with amniotic epithelium as compared to AM without amniotic epithelium suggest an epithelial origin for these growth factors. We feel that EGF, KGF and HGF in particular might play important roles in ocular surface wound healing after AM transplantation.",
author = "Noriko Koizumi and Tsutomo Inatomi and Chie Sotozono and Fullwood, {Nigel J.} and Quantock, {Andrew J.} and Shigeru Kinoshita",
year = "2000",
doi = "10.1076/0271-3683(200003)20:3;1-9;FT173",
language = "English",
volume = "20",
pages = "173--177",
journal = "Current Eye Research",
issn = "1460-2202",
publisher = "Informa Healthcare",
number = "3",

}

RIS

TY - JOUR

T1 - Growth factor mRNA and protein in preserved human amniotic membrane.

AU - Koizumi, Noriko

AU - Inatomi, Tsutomo

AU - Sotozono, Chie

AU - Fullwood, Nigel J.

AU - Quantock, Andrew J.

AU - Kinoshita, Shigeru

PY - 2000

Y1 - 2000

N2 - Purpose. To investigate the expression of growth factor mRNA and the level of growth factor protein in preserved human amniotic membrane (AM). Methods. RT-PCR was used to examine the expression of mRNA for eight growth factors (EGF, TGF-a, KGF, HGF, bFGF, TGF-1, -2, -3) and two growth factor receptors (KGFR and HGFR) in human AM preserved at -80°C for one month. In addition, ELISAs were used to measure the protein concentrations of seven growth factors (EGF, TGF-a, KGF, HGF, bFGF, TGF-1, -2) in preserved human corneas and in AM both with and without amniotic epithelium. Results. RT-PCR revealed that human AM expresses mRNA for EGF, TGF-a, KGF, HGF, bFGF, TGF-1, -2, -3, KGFR and HGFR, while ELISAs showed that it contains EGF, TGF-a, KGF, HGF, bFGF, TGF-1, -2. AM without amniotic epithelium also contains all seven growth factors examined, however, in this tissue the protein levels of EGF, KGF, HGF and bFGF were found to be significantly lower than in native AM. Conclusions. Preserved human AM expresses mRNAs for a number of growth factors and contains several growth factor proteins that might benefit epithelialization after AM transplantation. High levels of EGF, KGF, HGF and bFGF in AM with amniotic epithelium as compared to AM without amniotic epithelium suggest an epithelial origin for these growth factors. We feel that EGF, KGF and HGF in particular might play important roles in ocular surface wound healing after AM transplantation.

AB - Purpose. To investigate the expression of growth factor mRNA and the level of growth factor protein in preserved human amniotic membrane (AM). Methods. RT-PCR was used to examine the expression of mRNA for eight growth factors (EGF, TGF-a, KGF, HGF, bFGF, TGF-1, -2, -3) and two growth factor receptors (KGFR and HGFR) in human AM preserved at -80°C for one month. In addition, ELISAs were used to measure the protein concentrations of seven growth factors (EGF, TGF-a, KGF, HGF, bFGF, TGF-1, -2) in preserved human corneas and in AM both with and without amniotic epithelium. Results. RT-PCR revealed that human AM expresses mRNA for EGF, TGF-a, KGF, HGF, bFGF, TGF-1, -2, -3, KGFR and HGFR, while ELISAs showed that it contains EGF, TGF-a, KGF, HGF, bFGF, TGF-1, -2. AM without amniotic epithelium also contains all seven growth factors examined, however, in this tissue the protein levels of EGF, KGF, HGF and bFGF were found to be significantly lower than in native AM. Conclusions. Preserved human AM expresses mRNAs for a number of growth factors and contains several growth factor proteins that might benefit epithelialization after AM transplantation. High levels of EGF, KGF, HGF and bFGF in AM with amniotic epithelium as compared to AM without amniotic epithelium suggest an epithelial origin for these growth factors. We feel that EGF, KGF and HGF in particular might play important roles in ocular surface wound healing after AM transplantation.

U2 - 10.1076/0271-3683(200003)20:3;1-9;FT173

DO - 10.1076/0271-3683(200003)20:3;1-9;FT173

M3 - Journal article

VL - 20

SP - 173

EP - 177

JO - Current Eye Research

JF - Current Eye Research

SN - 1460-2202

IS - 3

ER -