Home > Research > Publications & Outputs > Gut microbial community supplementation and red...

Electronic data

  • fiac147

    Final published version, 1.86 MB, PDF document

    Available under license: CC BY: Creative Commons Attribution 4.0 International License

Links

Text available via DOI:

View graph of relations

Gut microbial community supplementation and reduction modulates African armyworm susceptibility to a baculovirus

Research output: Contribution to Journal/MagazineJournal articlepeer-review

Published

Standard

Gut microbial community supplementation and reduction modulates African armyworm susceptibility to a baculovirus. / Donkersley, Philip; Rice, Annabel; Graham, Robert I et al.
In: FEMS Microbiology Ecology, Vol. 99, No. 1, fiac147, 31.01.2023.

Research output: Contribution to Journal/MagazineJournal articlepeer-review

Harvard

APA

Vancouver

Donkersley P, Rice A, Graham RI, Wilson K. Gut microbial community supplementation and reduction modulates African armyworm susceptibility to a baculovirus. FEMS Microbiology Ecology. 2023 Jan 31;99(1):fiac147. Epub 2022 Dec 6. doi: 10.1093/femsec/fiac147

Author

Bibtex

@article{5a4d6417a8c44fa99c404378f64efd51,
title = "Gut microbial community supplementation and reduction modulates African armyworm susceptibility to a baculovirus",
abstract = "Gut microbiota stimulates the immune system and inhibits pathogens, and thus, it is critical for disease prevention. Probiotics represent an effective alternative to antibiotics used for the therapy and prevention of bacterial diseases. Probiotic bacteria are commonly used in vertebrates, although their use in invertebrates is still rare. We manipulated the gut microbiome of the African Armyworm (Spodoptera exempta Walker) using antibiotics and field-collected frass, in an attempt to understand the interactions of the gut microbiome with the nucleopolyhedrovirus, SpexNPV. We found that S. exempta individuals with supplemented gut microbiome were significantly more resistant to SpexNPV, relative to those with a typical laboratory gut microbiome. Illumina MiSeq sequencing revealed the bacterial phyla in the S. exempta gut belonged to 28 different classes. Individuals with an increased abundance of Lactobacillales had a higher probability of surviving viral infection. In contrast, there was an increased abundance of Enterobacteriales and Pseudomonadales in individuals dying from viral infection, corresponding with decreased abundance of these two Orders in surviving caterpillars, suggesting a potential role for them in modulating the interaction between the host and its pathogen. These results have important implications for laboratory studies testing biopesticides.",
keywords = "African armyworm, Gut, Microbiome, Nucleopolyhedrovirus, symbiosis",
author = "Philip Donkersley and Annabel Rice and Graham, {Robert I} and Kenneth Wilson",
year = "2023",
month = jan,
day = "31",
doi = "10.1093/femsec/fiac147",
language = "English",
volume = "99",
journal = "FEMS Microbiology Ecology",
issn = "0168-6496",
publisher = "Wiley-Blackwell",
number = "1",

}

RIS

TY - JOUR

T1 - Gut microbial community supplementation and reduction modulates African armyworm susceptibility to a baculovirus

AU - Donkersley, Philip

AU - Rice, Annabel

AU - Graham, Robert I

AU - Wilson, Kenneth

PY - 2023/1/31

Y1 - 2023/1/31

N2 - Gut microbiota stimulates the immune system and inhibits pathogens, and thus, it is critical for disease prevention. Probiotics represent an effective alternative to antibiotics used for the therapy and prevention of bacterial diseases. Probiotic bacteria are commonly used in vertebrates, although their use in invertebrates is still rare. We manipulated the gut microbiome of the African Armyworm (Spodoptera exempta Walker) using antibiotics and field-collected frass, in an attempt to understand the interactions of the gut microbiome with the nucleopolyhedrovirus, SpexNPV. We found that S. exempta individuals with supplemented gut microbiome were significantly more resistant to SpexNPV, relative to those with a typical laboratory gut microbiome. Illumina MiSeq sequencing revealed the bacterial phyla in the S. exempta gut belonged to 28 different classes. Individuals with an increased abundance of Lactobacillales had a higher probability of surviving viral infection. In contrast, there was an increased abundance of Enterobacteriales and Pseudomonadales in individuals dying from viral infection, corresponding with decreased abundance of these two Orders in surviving caterpillars, suggesting a potential role for them in modulating the interaction between the host and its pathogen. These results have important implications for laboratory studies testing biopesticides.

AB - Gut microbiota stimulates the immune system and inhibits pathogens, and thus, it is critical for disease prevention. Probiotics represent an effective alternative to antibiotics used for the therapy and prevention of bacterial diseases. Probiotic bacteria are commonly used in vertebrates, although their use in invertebrates is still rare. We manipulated the gut microbiome of the African Armyworm (Spodoptera exempta Walker) using antibiotics and field-collected frass, in an attempt to understand the interactions of the gut microbiome with the nucleopolyhedrovirus, SpexNPV. We found that S. exempta individuals with supplemented gut microbiome were significantly more resistant to SpexNPV, relative to those with a typical laboratory gut microbiome. Illumina MiSeq sequencing revealed the bacterial phyla in the S. exempta gut belonged to 28 different classes. Individuals with an increased abundance of Lactobacillales had a higher probability of surviving viral infection. In contrast, there was an increased abundance of Enterobacteriales and Pseudomonadales in individuals dying from viral infection, corresponding with decreased abundance of these two Orders in surviving caterpillars, suggesting a potential role for them in modulating the interaction between the host and its pathogen. These results have important implications for laboratory studies testing biopesticides.

KW - African armyworm

KW - Gut

KW - Microbiome

KW - Nucleopolyhedrovirus

KW - symbiosis

U2 - 10.1093/femsec/fiac147

DO - 10.1093/femsec/fiac147

M3 - Journal article

C2 - 36473704

VL - 99

JO - FEMS Microbiology Ecology

JF - FEMS Microbiology Ecology

SN - 0168-6496

IS - 1

M1 - fiac147

ER -