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Heterogeneous catalysis mediated cofactor NADH regeneration for enzymatic reduction

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Heterogeneous catalysis mediated cofactor NADH regeneration for enzymatic reduction. / Wang, Xiaodong; Yiu, Humphrey H. P.
In: ACS Catalysis, Vol. 6, No. 3, 18.02.2016, p. 1880-1886.

Research output: Contribution to Journal/Magazine › Journal article › peer-review

Harvard

Wang, X & Yiu, HHP 2016, 'Heterogeneous catalysis mediated cofactor NADH regeneration for enzymatic reduction', ACS Catalysis, vol. 6, no. 3, pp. 1880-1886. https://doi.org/10.1021/acscatal.5b02820

APA

Wang, X., & Yiu, H. H. P. (2016). Heterogeneous catalysis mediated cofactor NADH regeneration for enzymatic reduction. ACS Catalysis, 6(3), 1880-1886. https://doi.org/10.1021/acscatal.5b02820

Vancouver

Wang X, Yiu HHP. Heterogeneous catalysis mediated cofactor NADH regeneration for enzymatic reduction. ACS Catalysis. 2016 Feb 18;6(3):1880-1886. doi: 10.1021/acscatal.5b02820

Author

Wang, Xiaodong ; Yiu, Humphrey H. P. / Heterogeneous catalysis mediated cofactor NADH regeneration for enzymatic reduction. In: ACS Catalysis. 2016 ; Vol. 6, No. 3. pp. 1880-1886.

Bibtex

@article{41a5e4d5b0c64b42907854a5c5e0e979,
title = "Heterogeneous catalysis mediated cofactor NADH regeneration for enzymatic reduction",
abstract = "Enzymatic reduction using oxidoreductases is important in commercial chemical production. This enzymatic action requires a cofactor (e.g., NADH) as a hydrogen source that is consumed during reaction and must be regenerated. We present, for the first time, an in situ NADH regeneration (NAD+ → NADH) using a heterogeneous catalyst (Pt/Al2O3) and H2 coupled with an enzymatic reduction. This regeneration system can be operated at ambient pressure where NADH yield and turnover frequency (TOF) increased with temperature (20–37 °C) and pH (4.0–9.9) delivering full selectivity to enzymatically active NADH. Cofactor regeneration by heterogeneous catalysis represents a cleaner (H+ as sole byproduct) alternative to current enzymatic and homogeneous (electro- and photo-) catalytic methods with the added benefit of facile catalyst separation. The viability of coupling cofactor regeneration with enzymatic (alcohol dehydrogenase, ADH) reaction is established in aldehyde reduction (propanal to propanol) where 100% alcohol yield was achieved. The potential of this hybrid inorganic–enzymatic system is further demonstrated in the continuous (fed-batch) conversion of propanal with catalyst (activity/selectivity) stability for up to 100 h.",
author = "Xiaodong Wang and Yiu, {Humphrey H. P.}",
year = "2016",
month = feb,
day = "18",
doi = "10.1021/acscatal.5b02820",
language = "English",
volume = "6",
pages = "1880--1886",
journal = "ACS Catalysis",
issn = "2155-5435",
publisher = "American Chemical Society",
number = "3",

}

RIS

TY - JOUR

T1 - Heterogeneous catalysis mediated cofactor NADH regeneration for enzymatic reduction

AU - Wang, Xiaodong

AU - Yiu, Humphrey H. P.

PY - 2016/2/18

Y1 - 2016/2/18

N2 - Enzymatic reduction using oxidoreductases is important in commercial chemical production. This enzymatic action requires a cofactor (e.g., NADH) as a hydrogen source that is consumed during reaction and must be regenerated. We present, for the first time, an in situ NADH regeneration (NAD+ → NADH) using a heterogeneous catalyst (Pt/Al2O3) and H2 coupled with an enzymatic reduction. This regeneration system can be operated at ambient pressure where NADH yield and turnover frequency (TOF) increased with temperature (20–37 °C) and pH (4.0–9.9) delivering full selectivity to enzymatically active NADH. Cofactor regeneration by heterogeneous catalysis represents a cleaner (H+ as sole byproduct) alternative to current enzymatic and homogeneous (electro- and photo-) catalytic methods with the added benefit of facile catalyst separation. The viability of coupling cofactor regeneration with enzymatic (alcohol dehydrogenase, ADH) reaction is established in aldehyde reduction (propanal to propanol) where 100% alcohol yield was achieved. The potential of this hybrid inorganic–enzymatic system is further demonstrated in the continuous (fed-batch) conversion of propanal with catalyst (activity/selectivity) stability for up to 100 h.

AB - Enzymatic reduction using oxidoreductases is important in commercial chemical production. This enzymatic action requires a cofactor (e.g., NADH) as a hydrogen source that is consumed during reaction and must be regenerated. We present, for the first time, an in situ NADH regeneration (NAD+ → NADH) using a heterogeneous catalyst (Pt/Al2O3) and H2 coupled with an enzymatic reduction. This regeneration system can be operated at ambient pressure where NADH yield and turnover frequency (TOF) increased with temperature (20–37 °C) and pH (4.0–9.9) delivering full selectivity to enzymatically active NADH. Cofactor regeneration by heterogeneous catalysis represents a cleaner (H+ as sole byproduct) alternative to current enzymatic and homogeneous (electro- and photo-) catalytic methods with the added benefit of facile catalyst separation. The viability of coupling cofactor regeneration with enzymatic (alcohol dehydrogenase, ADH) reaction is established in aldehyde reduction (propanal to propanol) where 100% alcohol yield was achieved. The potential of this hybrid inorganic–enzymatic system is further demonstrated in the continuous (fed-batch) conversion of propanal with catalyst (activity/selectivity) stability for up to 100 h.

U2 - 10.1021/acscatal.5b02820

DO - 10.1021/acscatal.5b02820

M3 - Journal article

VL - 6

SP - 1880

EP - 1886

JO - ACS Catalysis

JF - ACS Catalysis

SN - 2155-5435

IS - 3

ER -