Home > Research > Publications & Outputs > High-Throughput Screening of Microbial Isolates...

Electronic data


Text available via DOI:

View graph of relations

High-Throughput Screening of Microbial Isolates with Impact on Caenorhabditis elegans Health

Research output: Contribution to Journal/MagazineJournal articlepeer-review

Article numbere63860
<mark>Journal publication date</mark>28/04/2022
<mark>Journal</mark>Journal of Visualized Experiments
Issue number182
Publication StatusPublished
Early online date28/04/22
<mark>Original language</mark>English


With its small size, short lifespan, and easy genetics, Caenorhabditis elegans offers a convenient platform to study the impact of microbial isolates on host physiology. It also fluoresces in blue when dying, providing a convenient means of pinpointing death. This property has been exploited to develop high-throughput label-free C. elegans survival assays (LFASS). These involve time-lapse fluorescence recording of worm populations set in multiwell plates, from which population median time of death can be derived. The present study adopts the LFASS approach to screen multiple microbial isolates at once for the effects on C. elegans susceptibility to severe heat and oxidative stresses. Such microbial screening pipeline, which can notably be used to prescreen probiotics, using severe stress resistance as a proxy for host health is reported here. The protocol describes how to grow both C. elegans gut microbiota isolate collections and synchronous worm populations in multiwell arrays before combining them for the assays. The example provided covers the testing of 47 bacterial isolates and one control strain on two worm strains, in two stress assays in parallel. However, the approach pipeline is readily scalable and applicable to the screening of many other modalities. Thus, it provides a versatile setup to rapidly survey a multiparametric landscape of biological and biochemical conditions that impact C. elegans health.

Bibliographic note

Export Date: 26 May 2022