Research output: Contribution to Journal/Magazine › Journal article › peer-review
Research output: Contribution to Journal/Magazine › Journal article › peer-review
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TY - JOUR
T1 - High-throughput sequence analysis of variants of human cytomegalovirus strains Towne and AD169
AU - Bradley, Amanda J.
AU - Lurain, Nell S.
AU - Ghazal, Peter
AU - Trivedi, Urmi
AU - Cunningham, Charles
AU - Baluchova, Katarina
AU - Gatherer, Derek
AU - Wilkinson, Gavin W. G.
AU - Dargan, Derrick J.
AU - Davison, Andrew J.
PY - 2009/10
Y1 - 2009/10
N2 - The genomes of commonly used variants of human cytomegalovirus (HCMV) strains Towne and AD169 each contain a substantial mutation in which a region (U(L)/b') at the right end of the long unique region has been replaced by an inverted duplication of a region from the left end of the genome. Using high-throughput technology, we have sequenced HCMV strain Towne (ATCC VR-977) and confirmed the presence of two variants, one exhibiting the replacement in U(L)/b' and the other intact in this region. Both variants are mutated in genes RL13, UL1, UL40, UL130, US1 and US9. We have also sequenced a novel AD169 variant (varUC) that is intact in U(L)/b' except for a small deletion that affects genes UL144, UL142, UL141 and UL140. Like other AD169 variants, varUC is mutated in genes RL5A, RL13, UL36 and UL131A. A subpopulation of varUC contains an additional deletion affecting genes IRS1, US1 and US2.
AB - The genomes of commonly used variants of human cytomegalovirus (HCMV) strains Towne and AD169 each contain a substantial mutation in which a region (U(L)/b') at the right end of the long unique region has been replaced by an inverted duplication of a region from the left end of the genome. Using high-throughput technology, we have sequenced HCMV strain Towne (ATCC VR-977) and confirmed the presence of two variants, one exhibiting the replacement in U(L)/b' and the other intact in this region. Both variants are mutated in genes RL13, UL1, UL40, UL130, US1 and US9. We have also sequenced a novel AD169 variant (varUC) that is intact in U(L)/b' except for a small deletion that affects genes UL144, UL142, UL141 and UL140. Like other AD169 variants, varUC is mutated in genes RL5A, RL13, UL36 and UL131A. A subpopulation of varUC contains an additional deletion affecting genes IRS1, US1 and US2.
KW - Base Sequence
KW - Cytomegalovirus
KW - DNA, Viral
KW - Genetic Variation
KW - Genome, Viral
KW - Humans
KW - Mutation
U2 - 10.1099/vir.0.013250-0
DO - 10.1099/vir.0.013250-0
M3 - Journal article
C2 - 19553388
VL - 90
SP - 2375
EP - 2380
JO - Journal of General Virology
JF - Journal of General Virology
SN - 0022-1317
IS - 10
ER -