Human aggrecan keratan sulfate undergoes structural changes during adolescent development

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Human aggrecan keratan sulfate undergoes structural changes during adolescent development. / Brown, Gavin; Huckerby, T N; Bayliss, M T et al.
In: Journal of Biological Chemistry, Vol. 273, No. 41, 09.10.1998, p. 26408-26414.

Research output: Contribution to Journal/Magazine › Journal article › peer-review

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Brown, Gavin ; Huckerby, T N ; Bayliss, M T et al. / Human aggrecan keratan sulfate undergoes structural changes during adolescent development. In: Journal of Biological Chemistry. 1998 ; Vol. 273, No. 41. pp. 26408-26414.

Bibtex

@article{a588c0d3892546fcbc256af9731b5606,

title = "Human aggrecan keratan sulfate undergoes structural changes during adolescent development",

abstract = "Alkaline borohydride-reduced keratan sulfate chains were isolated from human articular cartilage aggrecan from individuals of various ages (0-85 years old). The chains were structurally characterized using 1H NMR spectroscopy, gel permeation chromatography, and oligosaccharide profiling (after digestion with the enzymes keratanase and keratanase II). The results show that from birth to early adolescence (0-9 years) the levels of alpha(1-3)-fucosylation, alpha(2-3)-sialylation, and galactose sulfation increase. Also, the weight-average molecular weight of the chains increases. During maturation (9-18 years) the levels of fucosylation and galactose sulfation continue to increase and alpha(2-6)-sialylation of the chains occurs. In adult life (18-85 years) there is little change in the weight-average molecular weight of the chains, and the levels of fucosylation, sialylation, and sulfation remain fairly constant.",

keywords = "Acetylglucosaminidase, Adolescent, Adult, Aged, Aged, 80 and over, Aggrecans, Carbohydrate Conformation, Carbohydrate Sequence, Extracellular Matrix Proteins, Fucose, Humans, Hydrolysis, Keratan Sulfate, Lectins, C-Type, Magnetic Resonance Spectroscopy, Middle Aged, Molecular Sequence Data, N-Acetylneuraminic Acid, Proteoglycans",

author = "Gavin Brown and Huckerby, {T N} and Bayliss, {M T} and Nieduszynski, {I A}",

year = "1998",

month = oct,

day = "9",

doi = "10.1074/jbc.273.41.26408",

language = "English",

volume = "273",

pages = "26408--26414",

journal = "Journal of Biological Chemistry",

issn = "0021-9258",

publisher = "American Society for Biochemistry and Molecular Biology Inc.",

number = "41",

}

RIS

TY - JOUR

T1 - Human aggrecan keratan sulfate undergoes structural changes during adolescent development

AU - Brown, Gavin

AU - Huckerby, T N

AU - Bayliss, M T

AU - Nieduszynski, I A

PY - 1998/10/9

Y1 - 1998/10/9

N2 - Alkaline borohydride-reduced keratan sulfate chains were isolated from human articular cartilage aggrecan from individuals of various ages (0-85 years old). The chains were structurally characterized using 1H NMR spectroscopy, gel permeation chromatography, and oligosaccharide profiling (after digestion with the enzymes keratanase and keratanase II). The results show that from birth to early adolescence (0-9 years) the levels of alpha(1-3)-fucosylation, alpha(2-3)-sialylation, and galactose sulfation increase. Also, the weight-average molecular weight of the chains increases. During maturation (9-18 years) the levels of fucosylation and galactose sulfation continue to increase and alpha(2-6)-sialylation of the chains occurs. In adult life (18-85 years) there is little change in the weight-average molecular weight of the chains, and the levels of fucosylation, sialylation, and sulfation remain fairly constant.

AB - Alkaline borohydride-reduced keratan sulfate chains were isolated from human articular cartilage aggrecan from individuals of various ages (0-85 years old). The chains were structurally characterized using 1H NMR spectroscopy, gel permeation chromatography, and oligosaccharide profiling (after digestion with the enzymes keratanase and keratanase II). The results show that from birth to early adolescence (0-9 years) the levels of alpha(1-3)-fucosylation, alpha(2-3)-sialylation, and galactose sulfation increase. Also, the weight-average molecular weight of the chains increases. During maturation (9-18 years) the levels of fucosylation and galactose sulfation continue to increase and alpha(2-6)-sialylation of the chains occurs. In adult life (18-85 years) there is little change in the weight-average molecular weight of the chains, and the levels of fucosylation, sialylation, and sulfation remain fairly constant.

KW - Acetylglucosaminidase

KW - Adolescent

KW - Adult

KW - Aged

KW - Aged, 80 and over

KW - Aggrecans

KW - Carbohydrate Conformation

KW - Carbohydrate Sequence

KW - Extracellular Matrix Proteins

KW - Fucose

KW - Humans

KW - Hydrolysis

KW - Keratan Sulfate

KW - Lectins, C-Type

KW - Magnetic Resonance Spectroscopy

KW - Middle Aged

KW - Molecular Sequence Data

KW - N-Acetylneuraminic Acid

KW - Proteoglycans

U2 - 10.1074/jbc.273.41.26408

DO - 10.1074/jbc.273.41.26408

M3 - Journal article

C2 - 9756873

VL - 273

SP - 26408

EP - 26414

JO - Journal of Biological Chemistry

JF - Journal of Biological Chemistry

SN - 0021-9258

IS - 41

ER -

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