Research output: Contribution to Journal/Magazine › Journal article › peer-review
Research output: Contribution to Journal/Magazine › Journal article › peer-review
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TY - JOUR
T1 - Human periodontal ligament cell sheets cultured on amniotic membrane substrate
AU - Adachi, K.
AU - Amemiya, T.
AU - Nakamura, T.
AU - Honjyo, K.
AU - Kumamoto, S.
AU - Yamamoto, T.
AU - Bentley, Adam
AU - Fullwood, Nigel
AU - Kinoshita, S.
AU - Kanamura, N.
PY - 2014/9
Y1 - 2014/9
N2 - ObjectivePeriodontal ligament (PDL) cells and their substrates play key roles in periodontal regeneration. However, there has been no report on the use of amniotic membrane (AM) as a substrate for culturing PDL cells. In the current study, we conducted an analysis of PDL cells cultivated on AM to determine the distribution of factors responsible for maintaining the characteristics of PDL.Materials and MethodsAmniotic membrane was obtained from women undergoing cesarean sections, whereas PDL tissue was obtained from human maxillary third molars. The harvested PDL cells were maintained in explant culture for three or four passages, following which they were cultured on AM.ResultsAfter 3 weeks of culture, the PDL cells had grown well on AM. Immunofluorescence showed that these cells were capable of proliferating and potentially maintaining their PDL-like properties. In addition, strong cell–cell adhesion structures, namely desmosomes and tight junctions, were shown to be present between cells. Electron microscopy images showed that the cultured PDL cells had differentiated and proliferated on AM with lateral conjugation and adhesion to AM.ConclusionWe conclude that AM may represent a suitable substrate for culturing PDL cells and that PDL cells cultured on AM show sheet formation.
AB - ObjectivePeriodontal ligament (PDL) cells and their substrates play key roles in periodontal regeneration. However, there has been no report on the use of amniotic membrane (AM) as a substrate for culturing PDL cells. In the current study, we conducted an analysis of PDL cells cultivated on AM to determine the distribution of factors responsible for maintaining the characteristics of PDL.Materials and MethodsAmniotic membrane was obtained from women undergoing cesarean sections, whereas PDL tissue was obtained from human maxillary third molars. The harvested PDL cells were maintained in explant culture for three or four passages, following which they were cultured on AM.ResultsAfter 3 weeks of culture, the PDL cells had grown well on AM. Immunofluorescence showed that these cells were capable of proliferating and potentially maintaining their PDL-like properties. In addition, strong cell–cell adhesion structures, namely desmosomes and tight junctions, were shown to be present between cells. Electron microscopy images showed that the cultured PDL cells had differentiated and proliferated on AM with lateral conjugation and adhesion to AM.ConclusionWe conclude that AM may represent a suitable substrate for culturing PDL cells and that PDL cells cultured on AM show sheet formation.
KW - periodontal ligament cell
KW - amniotic membrane
KW - tissue engineering
U2 - 10.1111/odi.12176
DO - 10.1111/odi.12176
M3 - Journal article
C2 - 24112848
VL - 20
SP - 582
EP - 590
JO - Oral Diseases
JF - Oral Diseases
SN - 1601-0825
IS - 6
ER -