Home > Research > Publications & Outputs > Identification of important amino acid replacem...

Research

Associated organisational units

Links

Text available via DOI:

  • https://doi.org/10.1101/075168

    Final published version

    Available under license: CC BY-NC-ND: Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License

Keywords

View graph of relations

Identification of important amino acid replacements in the 2013-2016 Ebola virus outbreak

Research output: Contribution to Journal/Magazine › Journal article

Published

Standard

Identification of important amino acid replacements in the 2013-2016 Ebola virus outbreak. / Olabode, Abayomi; Gatherer, Derek; Jiang, Xiaowei et al.
In: Biorxiv, 14.09.2016.

Research output: Contribution to Journal/Magazine › Journal article

Harvard

Olabode, A, Gatherer, D, Jiang, X, Matthews, D, Hiscox, J, Gunther, S, Carroll, M, Lovell, S & Robertson, D 2016, 'Identification of important amino acid replacements in the 2013-2016 Ebola virus outbreak', Biorxiv. https://doi.org/10.1101/075168

APA

Olabode, A., Gatherer, D., Jiang, X., Matthews, D., Hiscox, J., Gunther, S., Carroll, M., Lovell, S., & Robertson, D. (2016). Identification of important amino acid replacements in the 2013-2016 Ebola virus outbreak. Biorxiv. https://doi.org/10.1101/075168

Vancouver

Olabode A, Gatherer D, Jiang X, Matthews D, Hiscox J, Gunther S et al. Identification of important amino acid replacements in the 2013-2016 Ebola virus outbreak. Biorxiv. 2016 Sept 14. doi: 10.1101/075168

Author

Olabode, Abayomi ; Gatherer, Derek ; Jiang, Xiaowei et al. / Identification of important amino acid replacements in the 2013-2016 Ebola virus outbreak. In: Biorxiv. 2016.

Bibtex

@article{5343ab982bc744f6a5d9e228076d4a80,
title = "Identification of important amino acid replacements in the 2013-2016 Ebola virus outbreak",
abstract = "The phylogenetic relationships of Zaire ebolavirus have been intensively analysed over the course of the 2013-2016 outbreak. However, there has been limited consideration of the functional impact of this variation. Here we describe an analysis of the available sequence data in the context of protein structure and phylogenetic history. Amino acid replacements are rare and predicted to have minor effects on protein stability. Synonymous mutations greatly outnumber nonsynonymous mutations, and most of the latter fall into unstructured intrinsically disordered regions, indicating that purifying selection is the dominant mode of selective pressure. However, one replacement, occurring early in the outbreak in Gueckedou in Guinea on 31st March 2014 (alanine to valine at position 82 in the GP protein), is close to the site where the virus binds to the host receptor NPC1 and is located in the phylogenetic tree at the origin of the major B lineage of the outbreak. The functional and evolutionary evidence indicates this A82V change likely has consequences for EBOV's host specificity and hence adaptation to humans.",
keywords = "Ebola, Ebola virus disease, EVD, molecular evolution, epidemiology, bats, Zaire ebolavirus, protein",
author = "Abayomi Olabode and Derek Gatherer and Xiaowei Jiang and David Matthews and Julian Hiscox and Stephan Gunther and Miles Carroll and Simon Lovell and David Robertson",
year = "2016",
month = sep,
day = "14",
doi = "10.1101/075168",
language = "English",
journal = "Biorxiv",
publisher = "Cold Spring Harbor Laboratory Press",

}

RIS

TY - JOUR

T1 - Identification of important amino acid replacements in the 2013-2016 Ebola virus outbreak

AU - Olabode, Abayomi

AU - Gatherer, Derek

AU - Jiang, Xiaowei

AU - Matthews, David

AU - Hiscox, Julian

AU - Gunther, Stephan

AU - Carroll, Miles

AU - Lovell, Simon

AU - Robertson, David

PY - 2016/9/14

Y1 - 2016/9/14

N2 - The phylogenetic relationships of Zaire ebolavirus have been intensively analysed over the course of the 2013-2016 outbreak. However, there has been limited consideration of the functional impact of this variation. Here we describe an analysis of the available sequence data in the context of protein structure and phylogenetic history. Amino acid replacements are rare and predicted to have minor effects on protein stability. Synonymous mutations greatly outnumber nonsynonymous mutations, and most of the latter fall into unstructured intrinsically disordered regions, indicating that purifying selection is the dominant mode of selective pressure. However, one replacement, occurring early in the outbreak in Gueckedou in Guinea on 31st March 2014 (alanine to valine at position 82 in the GP protein), is close to the site where the virus binds to the host receptor NPC1 and is located in the phylogenetic tree at the origin of the major B lineage of the outbreak. The functional and evolutionary evidence indicates this A82V change likely has consequences for EBOV's host specificity and hence adaptation to humans.

AB - The phylogenetic relationships of Zaire ebolavirus have been intensively analysed over the course of the 2013-2016 outbreak. However, there has been limited consideration of the functional impact of this variation. Here we describe an analysis of the available sequence data in the context of protein structure and phylogenetic history. Amino acid replacements are rare and predicted to have minor effects on protein stability. Synonymous mutations greatly outnumber nonsynonymous mutations, and most of the latter fall into unstructured intrinsically disordered regions, indicating that purifying selection is the dominant mode of selective pressure. However, one replacement, occurring early in the outbreak in Gueckedou in Guinea on 31st March 2014 (alanine to valine at position 82 in the GP protein), is close to the site where the virus binds to the host receptor NPC1 and is located in the phylogenetic tree at the origin of the major B lineage of the outbreak. The functional and evolutionary evidence indicates this A82V change likely has consequences for EBOV's host specificity and hence adaptation to humans.

KW - Ebola

KW - Ebola virus disease

KW - EVD

KW - molecular evolution

KW - epidemiology

KW - bats

KW - Zaire ebolavirus

KW - protein

U2 - 10.1101/075168

DO - 10.1101/075168

M3 - Journal article

JO - Biorxiv

JF - Biorxiv

ER -