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Identification of Leaf Promoters for Use in Transgenic Wheat

Research output: Contribution to Journal/Magazine › Journal article › peer-review

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Identification of Leaf Promoters for Use in Transgenic Wheat. / Alotaibi, Saqer; Sparks, Caroline; Parry, Martin et al.
In: Plants, Vol. 7, No. 2, 28.03.2018.

Research output: Contribution to Journal/Magazine › Journal article › peer-review

Harvard

Alotaibi, S, Sparks, C, Parry, M, Simkin, A & Raines, C 2018, 'Identification of Leaf Promoters for Use in Transgenic Wheat', Plants, vol. 7, no. 2. https://doi.org/10.3390/plants7020027

APA

Alotaibi, S., Sparks, C., Parry, M., Simkin, A., & Raines, C. (2018). Identification of Leaf Promoters for Use in Transgenic Wheat. Plants, 7(2). https://doi.org/10.3390/plants7020027

Vancouver

Alotaibi S, Sparks C, Parry M, Simkin A, Raines C. Identification of Leaf Promoters for Use in Transgenic Wheat. Plants. 2018 Mar 28;7(2). doi: 10.3390/plants7020027

Author

Alotaibi, Saqer ; Sparks, Caroline ; Parry, Martin et al. / Identification of Leaf Promoters for Use in Transgenic Wheat. In: Plants. 2018 ; Vol. 7, No. 2.

Bibtex

@article{eb73bc723377434ba53955efa3bf3ba1,
title = "Identification of Leaf Promoters for Use in Transgenic Wheat",
abstract = "Wheat yields have plateaued in recent years and given the growing global population there is a pressing need to develop higher yielding varieties to meet future demand. Genetic manipulation of photosynthesis in elite wheat varieties offers the opportunity to significantly increase yields. However, the absence of a well-defined molecular tool-box of promoters to manipulate leaf processes in wheat hinders advancements in this area. Two promoters, one driving the expression of sedoheptulose-1,7-bisphosphatase (SBPase) and the other fructose-1,6-bisphosphate aldolase (FBPA) from Brachypodium distachyon were identified and cloned into a vector in front of the GUS reporter gene. Both promoters were shown to be functionally active in wheat in both transient assays and in stably transformed wheat plants. Analysis of the stable transformants of wheat (cv. Cadenza) showed that both promoters controlled gus expression throughout leaf development as well as in other green tissues. The availability of these promoters provides new tools for the expression of genes in transgenic wheat leaves and also paves the way for multigene manipulation of photosynthesis to improve yields. ",
keywords = "promoter, wheat, tissue specific, photosynthesis, reporter gene, yield",
author = "Saqer Alotaibi and Caroline Sparks and Martin Parry and Andrew Simkin and Christine Raines",
year = "2018",
month = mar,
day = "28",
doi = "10.3390/plants7020027",
language = "English",
volume = "7",
journal = "Plants",
issn = "2223-7747",
publisher = "MDPI AG",
number = "2",

}

RIS

TY - JOUR

T1 - Identification of Leaf Promoters for Use in Transgenic Wheat

AU - Alotaibi, Saqer

AU - Sparks, Caroline

AU - Parry, Martin

AU - Simkin, Andrew

AU - Raines, Christine

PY - 2018/3/28

Y1 - 2018/3/28

N2 - Wheat yields have plateaued in recent years and given the growing global population there is a pressing need to develop higher yielding varieties to meet future demand. Genetic manipulation of photosynthesis in elite wheat varieties offers the opportunity to significantly increase yields. However, the absence of a well-defined molecular tool-box of promoters to manipulate leaf processes in wheat hinders advancements in this area. Two promoters, one driving the expression of sedoheptulose-1,7-bisphosphatase (SBPase) and the other fructose-1,6-bisphosphate aldolase (FBPA) from Brachypodium distachyon were identified and cloned into a vector in front of the GUS reporter gene. Both promoters were shown to be functionally active in wheat in both transient assays and in stably transformed wheat plants. Analysis of the stable transformants of wheat (cv. Cadenza) showed that both promoters controlled gus expression throughout leaf development as well as in other green tissues. The availability of these promoters provides new tools for the expression of genes in transgenic wheat leaves and also paves the way for multigene manipulation of photosynthesis to improve yields.

AB - Wheat yields have plateaued in recent years and given the growing global population there is a pressing need to develop higher yielding varieties to meet future demand. Genetic manipulation of photosynthesis in elite wheat varieties offers the opportunity to significantly increase yields. However, the absence of a well-defined molecular tool-box of promoters to manipulate leaf processes in wheat hinders advancements in this area. Two promoters, one driving the expression of sedoheptulose-1,7-bisphosphatase (SBPase) and the other fructose-1,6-bisphosphate aldolase (FBPA) from Brachypodium distachyon were identified and cloned into a vector in front of the GUS reporter gene. Both promoters were shown to be functionally active in wheat in both transient assays and in stably transformed wheat plants. Analysis of the stable transformants of wheat (cv. Cadenza) showed that both promoters controlled gus expression throughout leaf development as well as in other green tissues. The availability of these promoters provides new tools for the expression of genes in transgenic wheat leaves and also paves the way for multigene manipulation of photosynthesis to improve yields.

KW - promoter

KW - wheat

KW - tissue specific

KW - photosynthesis

KW - reporter gene

KW - yield

U2 - 10.3390/plants7020027

DO - 10.3390/plants7020027

M3 - Journal article

VL - 7

JO - Plants

JF - Plants

SN - 2223-7747

IS - 2

ER -