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Identification of β-propeller phytase-encoding genes in culturable Paenibacillus and Bacillus spp. from the rhizosphere of pasture plants on volcanic soils

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Identification of β-propeller phytase-encoding genes in culturable Paenibacillus and Bacillus spp. from the rhizosphere of pasture plants on volcanic soils. / Jorquera, Milko A.; Crowley, David E.; Marschner, Petra et al.
In: FEMS Microbiology Ecology, Vol. 75, No. 1, 01.2011, p. 163-172.

Research output: Contribution to Journal/Magazine › Journal article › peer-review

Harvard

Jorquera, MA, Crowley, DE, Marschner, P, Greiner, R, Fernández, MT, Romero, D, Menezes-Blackburn, D & De La Luz Mora, M 2011, 'Identification of β-propeller phytase-encoding genes in culturable Paenibacillus and Bacillus spp. from the rhizosphere of pasture plants on volcanic soils', FEMS Microbiology Ecology, vol. 75, no. 1, pp. 163-172. https://doi.org/10.1111/j.1574-6941.2010.00995.x

APA

Jorquera, M. A., Crowley, D. E., Marschner, P., Greiner, R., Fernández, M. T., Romero, D., Menezes-Blackburn, D., & De La Luz Mora, M. (2011). Identification of β-propeller phytase-encoding genes in culturable Paenibacillus and Bacillus spp. from the rhizosphere of pasture plants on volcanic soils. FEMS Microbiology Ecology, 75(1), 163-172. https://doi.org/10.1111/j.1574-6941.2010.00995.x

Vancouver

Jorquera MA, Crowley DE, Marschner P, Greiner R, Fernández MT, Romero D et al. Identification of β-propeller phytase-encoding genes in culturable Paenibacillus and Bacillus spp. from the rhizosphere of pasture plants on volcanic soils. FEMS Microbiology Ecology. 2011 Jan;75(1):163-172. Epub 2011 Jan 1. doi: 10.1111/j.1574-6941.2010.00995.x

Author

Jorquera, Milko A. ; Crowley, David E. ; Marschner, Petra et al. / Identification of β-propeller phytase-encoding genes in culturable Paenibacillus and Bacillus spp. from the rhizosphere of pasture plants on volcanic soils. In: FEMS Microbiology Ecology. 2011 ; Vol. 75, No. 1. pp. 163-172.

Bibtex

@article{2286cfd11dee466f9faee8cd02e19214,
title = "Identification of β-propeller phytase-encoding genes in culturable Paenibacillus and Bacillus spp. from the rhizosphere of pasture plants on volcanic soils",
abstract = "Phytate is one of the most abundant sources of organic phosphorus (P) in soils, but must be mineralized by phytase-producing bacteria to release P for plant uptake. Microbial inoculants based on Bacillus spp. have been developed commercially, but few studies have evaluated the ecology of these bacteria in the rhizosphere or the types of enzymes that they produce. Here, we studied the diversity of aerobic endospore-forming bacteria (EFB) with the ability to mineralize phytate in the rhizosphere of pasture plants grown in volcanic soils of southern Chile. PCR methods were used to detect candidate phytase-encoding genes and to identify EFB bacteria that carry these genes. This study revealed that the phytate-degrading EFB populations of pasture plants included species of Paenibacillus and Bacillus, which carried genes encoding β-propeller phytase (BPP). Assays of enzymatic activity confirmed the ability of these rhizosphere isolates to degrade phytate. The phytase-encoding genes described here may prove valuable as molecular markers to evaluate the role of EFB in organic P mobilization in the rhizosphere.",
keywords = "6-Phytase, Agriculture, Amino Acid Sequence, Bacillus, Chile, Consensus Sequence, Genes, Bacterial, Molecular Sequence Data, Paenibacillus, Phosphorus, Phylogeny, Phytic Acid, Poaceae, Polymerase Chain Reaction, Protein Structure, Tertiary, Rhizosphere, Soil, Soil Microbiology",
author = "Jorquera, {Milko A.} and Crowley, {David E.} and Petra Marschner and Ralf Greiner and Fern{\'a}ndez, {Mar{\'i}a Teresa} and Daniela Romero and Daniel Menezes-Blackburn and {De La Luz Mora}, Mar{\'i}a",
note = "{\textcopyright} 2010 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. All rights reserved.",
year = "2011",
month = jan,
doi = "10.1111/j.1574-6941.2010.00995.x",
language = "English",
volume = "75",
pages = "163--172",
journal = "FEMS Microbiology Ecology",
issn = "0168-6496",
publisher = "Wiley-Blackwell",
number = "1",

}

RIS

TY - JOUR

T1 - Identification of β-propeller phytase-encoding genes in culturable Paenibacillus and Bacillus spp. from the rhizosphere of pasture plants on volcanic soils

AU - Jorquera, Milko A.

AU - Crowley, David E.

AU - Marschner, Petra

AU - Greiner, Ralf

AU - Fernández, María Teresa

AU - Romero, Daniela

AU - Menezes-Blackburn, Daniel

AU - De La Luz Mora, María

N1 - © 2010 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. All rights reserved.

PY - 2011/1

Y1 - 2011/1

N2 - Phytate is one of the most abundant sources of organic phosphorus (P) in soils, but must be mineralized by phytase-producing bacteria to release P for plant uptake. Microbial inoculants based on Bacillus spp. have been developed commercially, but few studies have evaluated the ecology of these bacteria in the rhizosphere or the types of enzymes that they produce. Here, we studied the diversity of aerobic endospore-forming bacteria (EFB) with the ability to mineralize phytate in the rhizosphere of pasture plants grown in volcanic soils of southern Chile. PCR methods were used to detect candidate phytase-encoding genes and to identify EFB bacteria that carry these genes. This study revealed that the phytate-degrading EFB populations of pasture plants included species of Paenibacillus and Bacillus, which carried genes encoding β-propeller phytase (BPP). Assays of enzymatic activity confirmed the ability of these rhizosphere isolates to degrade phytate. The phytase-encoding genes described here may prove valuable as molecular markers to evaluate the role of EFB in organic P mobilization in the rhizosphere.

AB - Phytate is one of the most abundant sources of organic phosphorus (P) in soils, but must be mineralized by phytase-producing bacteria to release P for plant uptake. Microbial inoculants based on Bacillus spp. have been developed commercially, but few studies have evaluated the ecology of these bacteria in the rhizosphere or the types of enzymes that they produce. Here, we studied the diversity of aerobic endospore-forming bacteria (EFB) with the ability to mineralize phytate in the rhizosphere of pasture plants grown in volcanic soils of southern Chile. PCR methods were used to detect candidate phytase-encoding genes and to identify EFB bacteria that carry these genes. This study revealed that the phytate-degrading EFB populations of pasture plants included species of Paenibacillus and Bacillus, which carried genes encoding β-propeller phytase (BPP). Assays of enzymatic activity confirmed the ability of these rhizosphere isolates to degrade phytate. The phytase-encoding genes described here may prove valuable as molecular markers to evaluate the role of EFB in organic P mobilization in the rhizosphere.

KW - 6-Phytase

KW - Agriculture

KW - Amino Acid Sequence

KW - Bacillus

KW - Chile

KW - Consensus Sequence

KW - Genes, Bacterial

KW - Molecular Sequence Data

KW - Paenibacillus

KW - Phosphorus

KW - Phylogeny

KW - Phytic Acid

KW - Poaceae

KW - Polymerase Chain Reaction

KW - Protein Structure, Tertiary

KW - Rhizosphere

KW - Soil

KW - Soil Microbiology

U2 - 10.1111/j.1574-6941.2010.00995.x

DO - 10.1111/j.1574-6941.2010.00995.x

M3 - Journal article

C2 - 21073489

VL - 75

SP - 163

EP - 172

JO - FEMS Microbiology Ecology

JF - FEMS Microbiology Ecology

SN - 0168-6496

IS - 1

ER -