Research output: Contribution to Journal/Magazine › Literature review › peer-review
Research output: Contribution to Journal/Magazine › Literature review › peer-review
}
TY - JOUR
T1 - Imaging sclera with hard X-ray microscopy
AU - Fullwood, Nigel J.
AU - Martin, Frank
AU - Bentley, Adam
AU - Lee, Jin Pyung
AU - Lee, Sang Joon
PY - 2011/7
Y1 - 2011/7
N2 - In this study, the organization of collagen fibrils within the sclera of the eye was investigated using the 7 keV hard X-ray microscope of the Pohang light source and compared to images from electron and atomic force microscopy. From the captured X-ray images, individual collagen fibrils were observed clearly in a spatial resolution much better than 100 nm, both in longitudinal sections and in transverse sections. Some of the collagen fibrils showed evidence of axial periodicity. In some regions of the samples, we could see cross-bridge like structures between adjacent collagen fibrils. The X-ray microscope also allowed the observation of keratocytes and the lamella structure of the scleral stroma. The X-ray microscope has some unique advantages in the nano-scale imaging of bio-samples relative to other established imaging techniques. (C) 2011 Elsevier Ltd. All rights reserved.
AB - In this study, the organization of collagen fibrils within the sclera of the eye was investigated using the 7 keV hard X-ray microscope of the Pohang light source and compared to images from electron and atomic force microscopy. From the captured X-ray images, individual collagen fibrils were observed clearly in a spatial resolution much better than 100 nm, both in longitudinal sections and in transverse sections. Some of the collagen fibrils showed evidence of axial periodicity. In some regions of the samples, we could see cross-bridge like structures between adjacent collagen fibrils. The X-ray microscope also allowed the observation of keratocytes and the lamella structure of the scleral stroma. The X-ray microscope has some unique advantages in the nano-scale imaging of bio-samples relative to other established imaging techniques. (C) 2011 Elsevier Ltd. All rights reserved.
KW - X-ray microscopy
KW - Synchrotron
KW - Sclera
KW - Collagen
KW - Proteoglycans
KW - OBSERVING TRANSPARENT SPECIMENS
KW - PHASE-CONTRAST
KW - ELECTRON-MICROSCOPY
KW - CORNEAL STROMA
KW - RESOLUTION
KW - COLLAGENS
KW - AGGRECAN
U2 - 10.1016/j.micron.2011.01.012
DO - 10.1016/j.micron.2011.01.012
M3 - Literature review
VL - 42
SP - 506
EP - 511
JO - Micron
JF - Micron
SN - 0968-4328
IS - 5
ER -