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Imaging sclera with hard X-ray microscopy

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Imaging sclera with hard X-ray microscopy. / Fullwood, Nigel J.; Martin, Frank; Bentley, Adam et al.
In: Micron, Vol. 42, No. 5, 07.2011, p. 506-511.

Research output: Contribution to Journal/MagazineLiterature reviewpeer-review

Harvard

Fullwood, NJ, Martin, F, Bentley, A, Lee, JP & Lee, SJ 2011, 'Imaging sclera with hard X-ray microscopy', Micron, vol. 42, no. 5, pp. 506-511. https://doi.org/10.1016/j.micron.2011.01.012

APA

Vancouver

Fullwood NJ, Martin F, Bentley A, Lee JP, Lee SJ. Imaging sclera with hard X-ray microscopy. Micron. 2011 Jul;42(5):506-511. doi: 10.1016/j.micron.2011.01.012

Author

Fullwood, Nigel J. ; Martin, Frank ; Bentley, Adam et al. / Imaging sclera with hard X-ray microscopy. In: Micron. 2011 ; Vol. 42, No. 5. pp. 506-511.

Bibtex

@article{96fcaab16ac946f3acc12dce74a1cc12,
title = "Imaging sclera with hard X-ray microscopy",
abstract = "In this study, the organization of collagen fibrils within the sclera of the eye was investigated using the 7 keV hard X-ray microscope of the Pohang light source and compared to images from electron and atomic force microscopy. From the captured X-ray images, individual collagen fibrils were observed clearly in a spatial resolution much better than 100 nm, both in longitudinal sections and in transverse sections. Some of the collagen fibrils showed evidence of axial periodicity. In some regions of the samples, we could see cross-bridge like structures between adjacent collagen fibrils. The X-ray microscope also allowed the observation of keratocytes and the lamella structure of the scleral stroma. The X-ray microscope has some unique advantages in the nano-scale imaging of bio-samples relative to other established imaging techniques. (C) 2011 Elsevier Ltd. All rights reserved.",
keywords = "X-ray microscopy, Synchrotron, Sclera, Collagen, Proteoglycans, OBSERVING TRANSPARENT SPECIMENS, PHASE-CONTRAST, ELECTRON-MICROSCOPY, CORNEAL STROMA, RESOLUTION, COLLAGENS, AGGRECAN",
author = "Fullwood, {Nigel J.} and Frank Martin and Adam Bentley and Lee, {Jin Pyung} and Lee, {Sang Joon}",
year = "2011",
month = jul,
doi = "10.1016/j.micron.2011.01.012",
language = "English",
volume = "42",
pages = "506--511",
journal = "Micron",
issn = "0968-4328",
publisher = "Elsevier Limited",
number = "5",

}

RIS

TY - JOUR

T1 - Imaging sclera with hard X-ray microscopy

AU - Fullwood, Nigel J.

AU - Martin, Frank

AU - Bentley, Adam

AU - Lee, Jin Pyung

AU - Lee, Sang Joon

PY - 2011/7

Y1 - 2011/7

N2 - In this study, the organization of collagen fibrils within the sclera of the eye was investigated using the 7 keV hard X-ray microscope of the Pohang light source and compared to images from electron and atomic force microscopy. From the captured X-ray images, individual collagen fibrils were observed clearly in a spatial resolution much better than 100 nm, both in longitudinal sections and in transverse sections. Some of the collagen fibrils showed evidence of axial periodicity. In some regions of the samples, we could see cross-bridge like structures between adjacent collagen fibrils. The X-ray microscope also allowed the observation of keratocytes and the lamella structure of the scleral stroma. The X-ray microscope has some unique advantages in the nano-scale imaging of bio-samples relative to other established imaging techniques. (C) 2011 Elsevier Ltd. All rights reserved.

AB - In this study, the organization of collagen fibrils within the sclera of the eye was investigated using the 7 keV hard X-ray microscope of the Pohang light source and compared to images from electron and atomic force microscopy. From the captured X-ray images, individual collagen fibrils were observed clearly in a spatial resolution much better than 100 nm, both in longitudinal sections and in transverse sections. Some of the collagen fibrils showed evidence of axial periodicity. In some regions of the samples, we could see cross-bridge like structures between adjacent collagen fibrils. The X-ray microscope also allowed the observation of keratocytes and the lamella structure of the scleral stroma. The X-ray microscope has some unique advantages in the nano-scale imaging of bio-samples relative to other established imaging techniques. (C) 2011 Elsevier Ltd. All rights reserved.

KW - X-ray microscopy

KW - Synchrotron

KW - Sclera

KW - Collagen

KW - Proteoglycans

KW - OBSERVING TRANSPARENT SPECIMENS

KW - PHASE-CONTRAST

KW - ELECTRON-MICROSCOPY

KW - CORNEAL STROMA

KW - RESOLUTION

KW - COLLAGENS

KW - AGGRECAN

U2 - 10.1016/j.micron.2011.01.012

DO - 10.1016/j.micron.2011.01.012

M3 - Literature review

VL - 42

SP - 506

EP - 511

JO - Micron

JF - Micron

SN - 0968-4328

IS - 5

ER -