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Immune cells on the corneal endothelium of an allogeneic corneal transplantation rabbit model

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Immune cells on the corneal endothelium of an allogeneic corneal transplantation rabbit model. / Koudouna, Elena; Okumura, Naoki; Okazaki, Yugo et al.
In: Investigative Ophthalmology and Visual Science, Vol. 58, No. 1, 01.01.2017, p. 242-251.

Research output: Contribution to Journal/MagazineJournal articlepeer-review

Harvard

Koudouna, E, Okumura, N, Okazaki, Y, Nakano, S, Inoue, R, Fullwood, NJ, Hori, J, Kinoshita, S & Koizumi, N 2017, 'Immune cells on the corneal endothelium of an allogeneic corneal transplantation rabbit model', Investigative Ophthalmology and Visual Science, vol. 58, no. 1, pp. 242-251. https://doi.org/10.1167/iovs.16-20019

APA

Koudouna, E., Okumura, N., Okazaki, Y., Nakano, S., Inoue, R., Fullwood, N. J., Hori, J., Kinoshita, S., & Koizumi, N. (2017). Immune cells on the corneal endothelium of an allogeneic corneal transplantation rabbit model. Investigative Ophthalmology and Visual Science, 58(1), 242-251. https://doi.org/10.1167/iovs.16-20019

Vancouver

Koudouna E, Okumura N, Okazaki Y, Nakano S, Inoue R, Fullwood NJ et al. Immune cells on the corneal endothelium of an allogeneic corneal transplantation rabbit model. Investigative Ophthalmology and Visual Science. 2017 Jan 1;58(1):242-251. doi: 10.1167/iovs.16-20019

Author

Koudouna, Elena ; Okumura, Naoki ; Okazaki, Yugo et al. / Immune cells on the corneal endothelium of an allogeneic corneal transplantation rabbit model. In: Investigative Ophthalmology and Visual Science. 2017 ; Vol. 58, No. 1. pp. 242-251.

Bibtex

@article{08e233c5ebc74fd18e8ecee9050ac538,
title = "Immune cells on the corneal endothelium of an allogeneic corneal transplantation rabbit model",
abstract = "PURPOSE. Corneal endothelial cell density undergoes a progressive decrease for many years after transplantation, eventually threatening patients with late endothelial failure. The purpose of this study was to investigate the possibility of an immunologic response in successfully grafted corneal endothelium. METHODS. The corneal endothelium of patients who had undergone corneal transplantation was evaluated by specular microscopy. Rabbit models were subjected to penetrating keratoplasty (PK) with either syngeneic or allogeneic corneal transplants and Descemet{\textquoteright}s stripping endothelial keratoplasty (DSEK) with allogeneic corneal transplants. The presence of immune cells and expression of proinflammatory cytokines were determined by immunostaining. The corneal endothelium and immune cells were also evaluated by scanning electron microscopy. RESULTS. Scanning slit contact specular microscopy of patients with no features of graft rejection revealed cell-like white dots on the grafted corneal endothelium. The corneal endothelium of the allogeneic PK and DSEK rabbit models displayed the presence of immune cells, including CD4+ T-helper cells, CD8+ cytotoxic T cells, CD20+ B lymphocytes, CD68+ macrophages, and neutrophils, but these immune cells were rarely observed in the syngeneic PK model. These immune cells also produced proinflammatory cytokines. Notably, some of the corneal endothelial cells situated near these immune cells exhibited features of apoptosis. CONCLUSIONS. T lymphocytes, B lymphocytes, macrophages, and neutrophils are present on the grafted corneal endothelium in both PK and DSEK allogeneic rabbit models. The potential involvement of immune cells as an underlying pathophysiology for late endothelial failure deserves further examination.",
keywords = "Corneal endothelium, Immune cells, Inflammation",
author = "Elena Koudouna and Naoki Okumura and Yugo Okazaki and Shinichiro Nakano and Ryota Inoue and Fullwood, {Nigel J.} and Junko Hori and Shigeru Kinoshita and Noriko Koizumi",
year = "2017",
month = jan,
day = "1",
doi = "10.1167/iovs.16-20019",
language = "English",
volume = "58",
pages = "242--251",
journal = "Investigative Ophthalmology and Visual Science",
issn = "0146-0404",
publisher = "ASSOC RESEARCH VISION OPHTHALMOLOGY INC",
number = "1",

}

RIS

TY - JOUR

T1 - Immune cells on the corneal endothelium of an allogeneic corneal transplantation rabbit model

AU - Koudouna, Elena

AU - Okumura, Naoki

AU - Okazaki, Yugo

AU - Nakano, Shinichiro

AU - Inoue, Ryota

AU - Fullwood, Nigel J.

AU - Hori, Junko

AU - Kinoshita, Shigeru

AU - Koizumi, Noriko

PY - 2017/1/1

Y1 - 2017/1/1

N2 - PURPOSE. Corneal endothelial cell density undergoes a progressive decrease for many years after transplantation, eventually threatening patients with late endothelial failure. The purpose of this study was to investigate the possibility of an immunologic response in successfully grafted corneal endothelium. METHODS. The corneal endothelium of patients who had undergone corneal transplantation was evaluated by specular microscopy. Rabbit models were subjected to penetrating keratoplasty (PK) with either syngeneic or allogeneic corneal transplants and Descemet’s stripping endothelial keratoplasty (DSEK) with allogeneic corneal transplants. The presence of immune cells and expression of proinflammatory cytokines were determined by immunostaining. The corneal endothelium and immune cells were also evaluated by scanning electron microscopy. RESULTS. Scanning slit contact specular microscopy of patients with no features of graft rejection revealed cell-like white dots on the grafted corneal endothelium. The corneal endothelium of the allogeneic PK and DSEK rabbit models displayed the presence of immune cells, including CD4+ T-helper cells, CD8+ cytotoxic T cells, CD20+ B lymphocytes, CD68+ macrophages, and neutrophils, but these immune cells were rarely observed in the syngeneic PK model. These immune cells also produced proinflammatory cytokines. Notably, some of the corneal endothelial cells situated near these immune cells exhibited features of apoptosis. CONCLUSIONS. T lymphocytes, B lymphocytes, macrophages, and neutrophils are present on the grafted corneal endothelium in both PK and DSEK allogeneic rabbit models. The potential involvement of immune cells as an underlying pathophysiology for late endothelial failure deserves further examination.

AB - PURPOSE. Corneal endothelial cell density undergoes a progressive decrease for many years after transplantation, eventually threatening patients with late endothelial failure. The purpose of this study was to investigate the possibility of an immunologic response in successfully grafted corneal endothelium. METHODS. The corneal endothelium of patients who had undergone corneal transplantation was evaluated by specular microscopy. Rabbit models were subjected to penetrating keratoplasty (PK) with either syngeneic or allogeneic corneal transplants and Descemet’s stripping endothelial keratoplasty (DSEK) with allogeneic corneal transplants. The presence of immune cells and expression of proinflammatory cytokines were determined by immunostaining. The corneal endothelium and immune cells were also evaluated by scanning electron microscopy. RESULTS. Scanning slit contact specular microscopy of patients with no features of graft rejection revealed cell-like white dots on the grafted corneal endothelium. The corneal endothelium of the allogeneic PK and DSEK rabbit models displayed the presence of immune cells, including CD4+ T-helper cells, CD8+ cytotoxic T cells, CD20+ B lymphocytes, CD68+ macrophages, and neutrophils, but these immune cells were rarely observed in the syngeneic PK model. These immune cells also produced proinflammatory cytokines. Notably, some of the corneal endothelial cells situated near these immune cells exhibited features of apoptosis. CONCLUSIONS. T lymphocytes, B lymphocytes, macrophages, and neutrophils are present on the grafted corneal endothelium in both PK and DSEK allogeneic rabbit models. The potential involvement of immune cells as an underlying pathophysiology for late endothelial failure deserves further examination.

KW - Corneal endothelium

KW - Immune cells

KW - Inflammation

U2 - 10.1167/iovs.16-20019

DO - 10.1167/iovs.16-20019

M3 - Journal article

AN - SCOPUS:85010919216

VL - 58

SP - 242

EP - 251

JO - Investigative Ophthalmology and Visual Science

JF - Investigative Ophthalmology and Visual Science

SN - 0146-0404

IS - 1

ER -