Immuno-electron microscopic localisation of caveolin 1 in human placenta

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Immuno-electron microscopic localisation of caveolin 1 in human placenta. / Byrne, Simon; Ahenkorah, John; Hottor, Bismarck et al.
In: Immunobiology, Vol. 212, No. 1, 05.03.2007, p. 39-46.

Research output: Contribution to Journal/Magazine › Journal article › peer-review

Harvard

Byrne, S, Ahenkorah, J, Hottor, B, Lockwood, C & Ockleford, CD 2007, 'Immuno-electron microscopic localisation of caveolin 1 in human placenta', Immunobiology, vol. 212, no. 1, pp. 39-46. https://doi.org/10.1016/j.imbio.2006.09.002

APA

Byrne, S., Ahenkorah, J., Hottor, B., Lockwood, C., & Ockleford, C. D. (2007). Immuno-electron microscopic localisation of caveolin 1 in human placenta. Immunobiology, 212(1), 39-46. https://doi.org/10.1016/j.imbio.2006.09.002

Vancouver

Byrne S, Ahenkorah J, Hottor B, Lockwood C, Ockleford CD. Immuno-electron microscopic localisation of caveolin 1 in human placenta. Immunobiology. 2007 Mar 5;212(1):39-46. doi: 10.1016/j.imbio.2006.09.002

Author

Byrne, Simon ; Ahenkorah, John ; Hottor, Bismarck et al. / Immuno-electron microscopic localisation of caveolin 1 in human placenta. In: Immunobiology. 2007 ; Vol. 212, No. 1. pp. 39-46.

Bibtex

@article{aa604b4aefe64109b2c27054d6b0d5c6,

title = "Immuno-electron microscopic localisation of caveolin 1 in human placenta",

abstract = "We have localised the placental endothelial marker caveolin-1 at the ultrastructural level using indirect immunogold labelling. The particulate label has been quantified to assess the distribution of the target protein within term placental chorionic villi. The mesodermal compartment of the tissue was more heavily labelled than the ectodermally derived trophoblast. Basal plate lining endothelium, and villous endothelium had similar immunoreactivity with anti-caveolin-1 antibody. A polarised distribution of the caveolin within chorionic villous capillary endothelial cells was observed. As evidenced by immuno-reactivity, the protein was statistically significantly more concentrated in the region associated with the basal membrane than the apical membrane. The latter region contained in turn significantly more anticaveolin-1 immunoreactivity than the central region. These differences are discussed in the light of possible transport and signalling platform roles for villous and basal plate endothelium. (c) 2006 Elsevier GmbH. All rights reserved.",

keywords = "caveolin-1, chorionic villi, gold labelling, materno-foetal interaction, placental transport, signalling, NITRIC-OXIDE SYNTHASE, LASER-SCANNING MICROSCOPY, ULTRATHIN CRYOSECTIONS, ENDOTHELIAL-CELLS, IN-VIVO, IMMUNOFLUORESCENCE, TROPHOBLAST, EXPRESSION",

author = "Simon Byrne and John Ahenkorah and Bismarck Hottor and C. Lockwood and Ockleford, {Colin D.}",

year = "2007",

month = mar,

day = "5",

doi = "10.1016/j.imbio.2006.09.002",

language = "English",

volume = "212",

pages = "39--46",

journal = "Immunobiology",

issn = "0171-2985",

publisher = "Urban und Fischer Verlag GmbH und Co. KG",

number = "1",

}

RIS

TY - JOUR

T1 - Immuno-electron microscopic localisation of caveolin 1 in human placenta

AU - Byrne, Simon

AU - Ahenkorah, John

AU - Hottor, Bismarck

AU - Lockwood, C.

AU - Ockleford, Colin D.

PY - 2007/3/5

Y1 - 2007/3/5

N2 - We have localised the placental endothelial marker caveolin-1 at the ultrastructural level using indirect immunogold labelling. The particulate label has been quantified to assess the distribution of the target protein within term placental chorionic villi. The mesodermal compartment of the tissue was more heavily labelled than the ectodermally derived trophoblast. Basal plate lining endothelium, and villous endothelium had similar immunoreactivity with anti-caveolin-1 antibody. A polarised distribution of the caveolin within chorionic villous capillary endothelial cells was observed. As evidenced by immuno-reactivity, the protein was statistically significantly more concentrated in the region associated with the basal membrane than the apical membrane. The latter region contained in turn significantly more anticaveolin-1 immunoreactivity than the central region. These differences are discussed in the light of possible transport and signalling platform roles for villous and basal plate endothelium. (c) 2006 Elsevier GmbH. All rights reserved.

AB - We have localised the placental endothelial marker caveolin-1 at the ultrastructural level using indirect immunogold labelling. The particulate label has been quantified to assess the distribution of the target protein within term placental chorionic villi. The mesodermal compartment of the tissue was more heavily labelled than the ectodermally derived trophoblast. Basal plate lining endothelium, and villous endothelium had similar immunoreactivity with anti-caveolin-1 antibody. A polarised distribution of the caveolin within chorionic villous capillary endothelial cells was observed. As evidenced by immuno-reactivity, the protein was statistically significantly more concentrated in the region associated with the basal membrane than the apical membrane. The latter region contained in turn significantly more anticaveolin-1 immunoreactivity than the central region. These differences are discussed in the light of possible transport and signalling platform roles for villous and basal plate endothelium. (c) 2006 Elsevier GmbH. All rights reserved.

KW - caveolin-1

KW - chorionic villi

KW - gold labelling

KW - materno-foetal interaction

KW - placental transport

KW - signalling

KW - NITRIC-OXIDE SYNTHASE

KW - LASER-SCANNING MICROSCOPY

KW - ULTRATHIN CRYOSECTIONS

KW - ENDOTHELIAL-CELLS

KW - IN-VIVO

KW - IMMUNOFLUORESCENCE

KW - TROPHOBLAST

KW - EXPRESSION

U2 - 10.1016/j.imbio.2006.09.002

DO - 10.1016/j.imbio.2006.09.002

M3 - Journal article

VL - 212

SP - 39

EP - 46

JO - Immunobiology

JF - Immunobiology

SN - 0171-2985

IS - 1

ER -

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