Research output: Contribution to Journal/Magazine › Journal article › peer-review
Research output: Contribution to Journal/Magazine › Journal article › peer-review
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TY - JOUR
T1 - Immuno-electron microscopic localisation of caveolin 1 in human placenta
AU - Byrne, Simon
AU - Ahenkorah, John
AU - Hottor, Bismarck
AU - Lockwood, C.
AU - Ockleford, Colin D.
PY - 2007/3/5
Y1 - 2007/3/5
N2 - We have localised the placental endothelial marker caveolin-1 at the ultrastructural level using indirect immunogold labelling. The particulate label has been quantified to assess the distribution of the target protein within term placental chorionic villi. The mesodermal compartment of the tissue was more heavily labelled than the ectodermally derived trophoblast. Basal plate lining endothelium, and villous endothelium had similar immunoreactivity with anti-caveolin-1 antibody. A polarised distribution of the caveolin within chorionic villous capillary endothelial cells was observed. As evidenced by immuno-reactivity, the protein was statistically significantly more concentrated in the region associated with the basal membrane than the apical membrane. The latter region contained in turn significantly more anticaveolin-1 immunoreactivity than the central region. These differences are discussed in the light of possible transport and signalling platform roles for villous and basal plate endothelium. (c) 2006 Elsevier GmbH. All rights reserved.
AB - We have localised the placental endothelial marker caveolin-1 at the ultrastructural level using indirect immunogold labelling. The particulate label has been quantified to assess the distribution of the target protein within term placental chorionic villi. The mesodermal compartment of the tissue was more heavily labelled than the ectodermally derived trophoblast. Basal plate lining endothelium, and villous endothelium had similar immunoreactivity with anti-caveolin-1 antibody. A polarised distribution of the caveolin within chorionic villous capillary endothelial cells was observed. As evidenced by immuno-reactivity, the protein was statistically significantly more concentrated in the region associated with the basal membrane than the apical membrane. The latter region contained in turn significantly more anticaveolin-1 immunoreactivity than the central region. These differences are discussed in the light of possible transport and signalling platform roles for villous and basal plate endothelium. (c) 2006 Elsevier GmbH. All rights reserved.
KW - caveolin-1
KW - chorionic villi
KW - gold labelling
KW - materno-foetal interaction
KW - placental transport
KW - signalling
KW - NITRIC-OXIDE SYNTHASE
KW - LASER-SCANNING MICROSCOPY
KW - ULTRATHIN CRYOSECTIONS
KW - ENDOTHELIAL-CELLS
KW - IN-VIVO
KW - IMMUNOFLUORESCENCE
KW - TROPHOBLAST
KW - EXPRESSION
U2 - 10.1016/j.imbio.2006.09.002
DO - 10.1016/j.imbio.2006.09.002
M3 - Journal article
VL - 212
SP - 39
EP - 46
JO - Immunobiology
JF - Immunobiology
SN - 0171-2985
IS - 1
ER -