Rights statement: This is the author’s version of a work that was accepted for publication in Science of the Total Environment. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Science of the Total Environment, 811, 2022 DOI: 10.1016/j.scitotenv.2021.152243
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Research output: Contribution to Journal/Magazine › Journal article › peer-review
Research output: Contribution to Journal/Magazine › Journal article › peer-review
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TY - JOUR
T1 - Impact of lignocellulosic waste-immobilised white-rot fungi on enhancing the development of 14C-phenanthrene catabolism in soil
AU - Omoni, V.T.
AU - Ibeto, C.N.
AU - Lag-Brotons, A.J.
AU - Bankole, P.O.
AU - Semple, K.T.
N1 - This is the author’s version of a work that was accepted for publication in Science of the Total Environment. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Science of the Total Environment, 811, 2022 DOI: 10.1016/j.scitotenv.2021.152243
PY - 2022/3/10
Y1 - 2022/3/10
N2 - In this study, an investigation was carried out to explore the the impact of white-rot fungi (WRF) on enhancing the development of phenanthrene catabolism in soil over time (1, 25, 50, 75 and 100 d). The WRF were immobilised on spent brewery grains (SBG) prior to inoculation to the soil. The results showed that SBG-immobilised WRF-amended soils reduced the lag phases and increased the extents of 14C-phenanthrene mineralisation. Greater reductions in the lag phases and increases in the rates of mineralisation were observed in immobilised Trametes versicolor-amended soil compared to the other WRF-amendments. However, the presence of Pleurotus ostreatus and Phanerochaete chrysosporium influenced biodegradation more strongly than the other fungal species. In addition, fungal enzyme activities increased in the amended soils and positively correlated with the extents of 14C-phenanthrene mineralisation in all soil amendments. Maximum ligninolytic enzyme activities were observed in P. ostreatus-amended soil. Microbial populations increased in all amended soils while PAH-degrading fungal numbers increased with increased soil-PAH contact time and strongly positively correlated with fastest rates of mineralisation. The findings presented in this study demonstrate that inoculating the soil with these immobilised WRFs generally enhanced the mineralisation of the 14C-phenanthrene in soil. This has the potential to be used to stimulate or enhance PAH catabolism in field-contaminated soils.
AB - In this study, an investigation was carried out to explore the the impact of white-rot fungi (WRF) on enhancing the development of phenanthrene catabolism in soil over time (1, 25, 50, 75 and 100 d). The WRF were immobilised on spent brewery grains (SBG) prior to inoculation to the soil. The results showed that SBG-immobilised WRF-amended soils reduced the lag phases and increased the extents of 14C-phenanthrene mineralisation. Greater reductions in the lag phases and increases in the rates of mineralisation were observed in immobilised Trametes versicolor-amended soil compared to the other WRF-amendments. However, the presence of Pleurotus ostreatus and Phanerochaete chrysosporium influenced biodegradation more strongly than the other fungal species. In addition, fungal enzyme activities increased in the amended soils and positively correlated with the extents of 14C-phenanthrene mineralisation in all soil amendments. Maximum ligninolytic enzyme activities were observed in P. ostreatus-amended soil. Microbial populations increased in all amended soils while PAH-degrading fungal numbers increased with increased soil-PAH contact time and strongly positively correlated with fastest rates of mineralisation. The findings presented in this study demonstrate that inoculating the soil with these immobilised WRFs generally enhanced the mineralisation of the 14C-phenanthrene in soil. This has the potential to be used to stimulate or enhance PAH catabolism in field-contaminated soils.
KW - Enzymes
KW - Immobilisation
KW - Lignocellulose
KW - Phenanthrene
KW - Soil
KW - White-rot fungi
KW - Anthracene
KW - Biodegradation
KW - Enzyme immobilization
KW - Fungi
KW - Metabolism
KW - Mineralogy
KW - Soil pollution
KW - Soils
KW - Amended soil
KW - Enzymes activity
KW - Lag phase
KW - Lag phasis
KW - Lignocellulosic wastes
KW - Mineralisation
KW - Phenanthrene mineralization
KW - Spent brewery grains
KW - White rot fungi
KW - carbon
KW - carbon 14
KW - fungal enzyme
KW - lignocellulose
KW - nitrogen
KW - phenanthrene
KW - polycyclic aromatic hydrocarbon
KW - absorption lag time
KW - Article
KW - biodegradation
KW - Bjerkandera adusta
KW - catabolism
KW - contact time
KW - controlled study
KW - environmental impact
KW - enzyme activity
KW - fungus culture
KW - Irpex lateus
KW - microbial community
KW - mineralization
KW - nonhuman
KW - Phanerochaete
KW - physical phenomena
KW - Pleurotus ostreatus
KW - soil acidity
KW - soil inoculation
KW - soil pollution
KW - soil property
KW - Trametes versicolor
KW - waste management
KW - white rot fungus
U2 - 10.1016/j.scitotenv.2021.152243
DO - 10.1016/j.scitotenv.2021.152243
M3 - Journal article
VL - 811
JO - Science of the Total Environment
JF - Science of the Total Environment
SN - 0048-9697
M1 - 152243
ER -