Rights statement: This is the author’s version of a work that was accepted for publication in Soil Biology and Biochemistry. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Soil Biology and Biochemistry, 120, 2018 DOI: 10.1016/j.soilbio.2018.02.009
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Research output: Contribution to Journal/Magazine › Journal article › peer-review
Research output: Contribution to Journal/Magazine › Journal article › peer-review
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TY - JOUR
T1 - Impact of single and binary mixtures of phenanthrene and N-PAHs on microbial utilization of 14C-glucose in soil
AU - Anyanwu, Ihuoma N.
AU - Semple, Kirk T.
N1 - This is the author’s version of a work that was accepted for publication in Soil Biology and Biochemistry. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Soil Biology and Biochemistry, 120, 2018 DOI: 10.1016/j.soilbio.2018.02.009
PY - 2018/5
Y1 - 2018/5
N2 - Microbes are susceptible to contaminant effects, and high concentration of chemicals in soil can impact on microbial growth, density, viability and development. The impact of single and binary mixtures of phenanthrene and its nitrogen-containing polycyclic aromatic hydrocarbon analogues (N-PAHs) on microbial metabolism of 14C-glucose in soil was measured over a 90 d soil-contact time. Impacts were assessed by measuring the rates and mean overall extents of mineralisation (%), as well as the incorporation of 14C-glucose into the microbial biomass. The result revealed that the extents of 14C-glucose mineralisation were consistently greater in N-PAH amended soils than the control and phenanthrene soils with increased incubations. This indicates a trend of increasing diversion of C from biosynthesis to maintenance requirement by soil microorganisms. Furthermore, biomass uptake in the amended soils showed reduced substrate utilization (fixed-kEC), suggesting that N-PAHs decreased the amount of substrate-C that was incorporated into the microbial biomass. This however, signifies that N-PAHs imposes oxidative stress on soil microbial community.
AB - Microbes are susceptible to contaminant effects, and high concentration of chemicals in soil can impact on microbial growth, density, viability and development. The impact of single and binary mixtures of phenanthrene and its nitrogen-containing polycyclic aromatic hydrocarbon analogues (N-PAHs) on microbial metabolism of 14C-glucose in soil was measured over a 90 d soil-contact time. Impacts were assessed by measuring the rates and mean overall extents of mineralisation (%), as well as the incorporation of 14C-glucose into the microbial biomass. The result revealed that the extents of 14C-glucose mineralisation were consistently greater in N-PAH amended soils than the control and phenanthrene soils with increased incubations. This indicates a trend of increasing diversion of C from biosynthesis to maintenance requirement by soil microorganisms. Furthermore, biomass uptake in the amended soils showed reduced substrate utilization (fixed-kEC), suggesting that N-PAHs decreased the amount of substrate-C that was incorporated into the microbial biomass. This however, signifies that N-PAHs imposes oxidative stress on soil microbial community.
KW - N-PAHs
KW - Phenanthrene
KW - 14C-biomass uptake
KW - Mineralisation
KW - kEC coefficient
U2 - 10.1016/j.soilbio.2018.02.009
DO - 10.1016/j.soilbio.2018.02.009
M3 - Journal article
VL - 120
SP - 222
EP - 229
JO - Soil Biology and Biochemistry
JF - Soil Biology and Biochemistry
SN - 0038-0717
ER -