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    Rights statement: http://journals.cambridge.org/action/displayJournal?jid=PAR The final, definitive version of this article has been published in the Journal, Parasitology, 116 (4), pp 305-309 1998, © 1998 Cambridge University Press.

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In vitro stimulation of metacyclogenesis in Leishmania braziliensis, L. donovani, L. major and L. mexicana

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In vitro stimulation of metacyclogenesis in Leishmania braziliensis, L. donovani, L. major and L. mexicana. / Zakai, H A; Chance, M L; Bates, P A.

In: Parasitology, Vol. 116 , No. 4, 04.1998, p. 305-309.

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Zakai, H A ; Chance, M L ; Bates, P A. / In vitro stimulation of metacyclogenesis in Leishmania braziliensis, L. donovani, L. major and L. mexicana. In: Parasitology. 1998 ; Vol. 116 , No. 4. pp. 305-309.

Bibtex

@article{7d65a58e7e034ddcb133fe757d50393f,
title = "In vitro stimulation of metacyclogenesis in Leishmania braziliensis, L. donovani, L. major and L. mexicana",
abstract = "Promastigotes of Leishmania braziliensis, L. donovani, L. major and L. mexicana recently derived from tissue amastigotes were cultured in Schneider's Drosophila medium supplemented with 20% (v/v) heat-inactivated foetal calf serum and 25 micrograms gentamicin sulfate/ml at pH 5.5. These cultures produced more metacyclic promastigotes in their stationary-phase populations than others cultured at pH 7.0. Metacyclic promastigotes possessed a short (<or = 8 microns) and narrow (<or = 1.5 microns) cell body with a flagellum twice or more the length of the cell body. Promastigotes from acidic cultures were more resistant to complement-mediated lysis and more infective in vivo than those grown at neutral pH. These results demonstrate that induction of metacyclogenesis by acidic pH is a response conserved across a variety of species of Leishmania.",
keywords = "Leishmania spp, promastigote , metacyclic , culture , infectivity , differentiation",
author = "Zakai, {H A} and Chance, {M L} and Bates, {P A}",
note = "http://journals.cambridge.org/action/displayJournal?jid=PAR The final, definitive version of this article has been published in the Journal, Parasitology, 116 (4), pp 305-309 1998, {\textcopyright} 1998 Cambridge University Press.",
year = "1998",
month = apr,
language = "English",
volume = "116 ",
pages = "305--309",
journal = "Parasitology",
issn = "0031-1820",
publisher = "Cambridge University Press",
number = "4",

}

RIS

TY - JOUR

T1 - In vitro stimulation of metacyclogenesis in Leishmania braziliensis, L. donovani, L. major and L. mexicana

AU - Zakai, H A

AU - Chance, M L

AU - Bates, P A

N1 - http://journals.cambridge.org/action/displayJournal?jid=PAR The final, definitive version of this article has been published in the Journal, Parasitology, 116 (4), pp 305-309 1998, © 1998 Cambridge University Press.

PY - 1998/4

Y1 - 1998/4

N2 - Promastigotes of Leishmania braziliensis, L. donovani, L. major and L. mexicana recently derived from tissue amastigotes were cultured in Schneider's Drosophila medium supplemented with 20% (v/v) heat-inactivated foetal calf serum and 25 micrograms gentamicin sulfate/ml at pH 5.5. These cultures produced more metacyclic promastigotes in their stationary-phase populations than others cultured at pH 7.0. Metacyclic promastigotes possessed a short (<or = 8 microns) and narrow (<or = 1.5 microns) cell body with a flagellum twice or more the length of the cell body. Promastigotes from acidic cultures were more resistant to complement-mediated lysis and more infective in vivo than those grown at neutral pH. These results demonstrate that induction of metacyclogenesis by acidic pH is a response conserved across a variety of species of Leishmania.

AB - Promastigotes of Leishmania braziliensis, L. donovani, L. major and L. mexicana recently derived from tissue amastigotes were cultured in Schneider's Drosophila medium supplemented with 20% (v/v) heat-inactivated foetal calf serum and 25 micrograms gentamicin sulfate/ml at pH 5.5. These cultures produced more metacyclic promastigotes in their stationary-phase populations than others cultured at pH 7.0. Metacyclic promastigotes possessed a short (<or = 8 microns) and narrow (<or = 1.5 microns) cell body with a flagellum twice or more the length of the cell body. Promastigotes from acidic cultures were more resistant to complement-mediated lysis and more infective in vivo than those grown at neutral pH. These results demonstrate that induction of metacyclogenesis by acidic pH is a response conserved across a variety of species of Leishmania.

KW - Leishmania spp

KW - promastigote

KW - metacyclic

KW - culture

KW - infectivity

KW - differentiation

M3 - Journal article

C2 - 9585932

VL - 116

SP - 305

EP - 309

JO - Parasitology

JF - Parasitology

SN - 0031-1820

IS - 4

ER -