Home > Research > Publications & Outputs > Infrared spectroscopy detects changes in an amp...

Electronic data

  • AqTox2016

    Rights statement: This is the author’s version of a work that was accepted for publication in Aquatic Toxicology. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Aquatic Toxicology, 178, 2016 DOI: 10.1016/j.aquatox.2016.07.005

    Accepted author manuscript, 849 KB, PDF document

    Available under license: CC BY-NC-ND: Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License

Links

Text available via DOI:

View graph of relations

Infrared spectroscopy detects changes in an amphibian cell line induced by fungicides: comparison of single and mixture effects

Research output: Contribution to Journal/MagazineJournal articlepeer-review

Published

Standard

Infrared spectroscopy detects changes in an amphibian cell line induced by fungicides: comparison of single and mixture effects . / Strong, Becky; Halsall, Crispin James; Jones, Kevin Christopher et al.
In: Aquatic Toxicology, Vol. 178, 09.2016, p. 8-18.

Research output: Contribution to Journal/MagazineJournal articlepeer-review

Harvard

APA

Vancouver

Author

Bibtex

@article{bdcd463809e04968a6d876349099d5e5,
title = "Infrared spectroscopy detects changes in an amphibian cell line induced by fungicides: comparison of single and mixture effects ",
abstract = "Amphibians are regarded as sensitive sentinels of environmental pollution due to their permeable skin and complex life cycle, which usually involves reproduction and development in the aquatic environment. Fungicides are widely applied agrochemicals and have been associated with developmental defects in amphibians; thus, it is important to determine chronic effects of environmentally-relevant concentrations of such contaminants in target cells. Infrared (IR) spectroscopy has been employed to signature the biological effects of environmental contaminants through extracting key features in IR spectra with chemometric methods. Herein, the Xenopus laevis (A6) cell line was exposed to low concentrations of carbendazim (a benzimidazole fungicide) or flusilazole (a triazole fungicide) either singly or as a binary mixture. Cells were then examined using attenuated total reflection Fourier-transform IR (ATR-FTIR) spectroscopy coupled with multivariate analysis. Results indicate significant changes in the IR spectra of cells induced by both agents at all concentrations following single exposures, primarily in regions associated with protein and phospholipids. Distinct differences were apparent in the IR spectra of cells exposed to carbendazim and those exposed to flusilazole, suggesting different mechanisms of action. Exposure to binary mixtures of carbendazim and flusilazole also induced significant spectral alterations, again in regions associated with phospholipids and proteins, but also in regions associated with DNA and carbohydrates. Overall these findings demonstrate that IR spectroscopy is a sensitive technique for examining the effects of environmentally-relevant levels of fungicides at the cellular level. The combination of IR spectroscopy with the A6 cell line could serve as a useful model to identify agents that might threaten amphibian health in a rapid and high throughput manner.",
keywords = "A6 cells, ATR-FTIR spectroscopy, Carbendazim, Flusilazole, Xenopus laevis",
author = "Becky Strong and Halsall, {Crispin James} and Jones, {Kevin Christopher} and Shore, {Richard Francis} and Martin, {Francis Luke}",
note = "This is the author{\textquoteright}s version of a work that was accepted for publication in Aquatic Toxicology. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Aquatic Toxicology, 178, 2016 DOI: 10.1016/j.aquatox.2016.07.005",
year = "2016",
month = sep,
doi = "10.1016/j.aquatox.2016.07.005",
language = "English",
volume = "178",
pages = "8--18",
journal = "Aquatic Toxicology",
issn = "1879-1514",
publisher = "Elsevier",

}

RIS

TY - JOUR

T1 - Infrared spectroscopy detects changes in an amphibian cell line induced by fungicides

T2 - comparison of single and mixture effects

AU - Strong, Becky

AU - Halsall, Crispin James

AU - Jones, Kevin Christopher

AU - Shore, Richard Francis

AU - Martin, Francis Luke

N1 - This is the author’s version of a work that was accepted for publication in Aquatic Toxicology. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Aquatic Toxicology, 178, 2016 DOI: 10.1016/j.aquatox.2016.07.005

PY - 2016/9

Y1 - 2016/9

N2 - Amphibians are regarded as sensitive sentinels of environmental pollution due to their permeable skin and complex life cycle, which usually involves reproduction and development in the aquatic environment. Fungicides are widely applied agrochemicals and have been associated with developmental defects in amphibians; thus, it is important to determine chronic effects of environmentally-relevant concentrations of such contaminants in target cells. Infrared (IR) spectroscopy has been employed to signature the biological effects of environmental contaminants through extracting key features in IR spectra with chemometric methods. Herein, the Xenopus laevis (A6) cell line was exposed to low concentrations of carbendazim (a benzimidazole fungicide) or flusilazole (a triazole fungicide) either singly or as a binary mixture. Cells were then examined using attenuated total reflection Fourier-transform IR (ATR-FTIR) spectroscopy coupled with multivariate analysis. Results indicate significant changes in the IR spectra of cells induced by both agents at all concentrations following single exposures, primarily in regions associated with protein and phospholipids. Distinct differences were apparent in the IR spectra of cells exposed to carbendazim and those exposed to flusilazole, suggesting different mechanisms of action. Exposure to binary mixtures of carbendazim and flusilazole also induced significant spectral alterations, again in regions associated with phospholipids and proteins, but also in regions associated with DNA and carbohydrates. Overall these findings demonstrate that IR spectroscopy is a sensitive technique for examining the effects of environmentally-relevant levels of fungicides at the cellular level. The combination of IR spectroscopy with the A6 cell line could serve as a useful model to identify agents that might threaten amphibian health in a rapid and high throughput manner.

AB - Amphibians are regarded as sensitive sentinels of environmental pollution due to their permeable skin and complex life cycle, which usually involves reproduction and development in the aquatic environment. Fungicides are widely applied agrochemicals and have been associated with developmental defects in amphibians; thus, it is important to determine chronic effects of environmentally-relevant concentrations of such contaminants in target cells. Infrared (IR) spectroscopy has been employed to signature the biological effects of environmental contaminants through extracting key features in IR spectra with chemometric methods. Herein, the Xenopus laevis (A6) cell line was exposed to low concentrations of carbendazim (a benzimidazole fungicide) or flusilazole (a triazole fungicide) either singly or as a binary mixture. Cells were then examined using attenuated total reflection Fourier-transform IR (ATR-FTIR) spectroscopy coupled with multivariate analysis. Results indicate significant changes in the IR spectra of cells induced by both agents at all concentrations following single exposures, primarily in regions associated with protein and phospholipids. Distinct differences were apparent in the IR spectra of cells exposed to carbendazim and those exposed to flusilazole, suggesting different mechanisms of action. Exposure to binary mixtures of carbendazim and flusilazole also induced significant spectral alterations, again in regions associated with phospholipids and proteins, but also in regions associated with DNA and carbohydrates. Overall these findings demonstrate that IR spectroscopy is a sensitive technique for examining the effects of environmentally-relevant levels of fungicides at the cellular level. The combination of IR spectroscopy with the A6 cell line could serve as a useful model to identify agents that might threaten amphibian health in a rapid and high throughput manner.

KW - A6 cells

KW - ATR-FTIR spectroscopy

KW - Carbendazim

KW - Flusilazole

KW - Xenopus laevis

U2 - 10.1016/j.aquatox.2016.07.005

DO - 10.1016/j.aquatox.2016.07.005

M3 - Journal article

VL - 178

SP - 8

EP - 18

JO - Aquatic Toxicology

JF - Aquatic Toxicology

SN - 1879-1514

ER -