Home > Research > Publications & Outputs > Interactions between cytoplasmic and nuclear ge...

Electronic data

  • epub_cytonuc_JEB (003)

    Rights statement: This is the peer reviewed version of the following article: Vaught, R.C., Voigt, S., Dobler, R., Clancy, D.J., Reinhardt, K. and Dowling, D.K. (2020), Interactions between cytoplasmic and nuclear genomes confer sex‐specific effects on lifespan in Drosophila melanogaster. J Evol Biol. doi:10.1111/jeb.13605 which has been published in final form at https://onlinelibrary.wiley.com/doi/10.1111/jeb.13605 This article may be used for non-commercial purposes in accordance With Wiley Terms and Conditions for self-archiving.

    Accepted author manuscript, 1.85 MB, PDF document

    Available under license: CC BY-NC: Creative Commons Attribution-NonCommercial 4.0 International License

Links

Text available via DOI:

View graph of relations

Interactions between cytoplasmic and nuclear genomes confer sex‐specific effects on lifespan in Drosophila melanogaster

Research output: Contribution to Journal/MagazineJournal articlepeer-review

Published

Standard

Interactions between cytoplasmic and nuclear genomes confer sex‐specific effects on lifespan in Drosophila melanogaster. / Vaught, Rebecca; Voigt, Suzanne; Dobler, Ralph et al.
In: Journal of Evolutionary Biology, Vol. 33, No. 5, 01.05.2020, p. 694-713.

Research output: Contribution to Journal/MagazineJournal articlepeer-review

Harvard

Vaught, R, Voigt, S, Dobler, R, Clancy, D, Reinhardt, K & Dowling, DK 2020, 'Interactions between cytoplasmic and nuclear genomes confer sex‐specific effects on lifespan in Drosophila melanogaster', Journal of Evolutionary Biology, vol. 33, no. 5, pp. 694-713. https://doi.org/10.1111/jeb.13605

APA

Vaught, R., Voigt, S., Dobler, R., Clancy, D., Reinhardt, K., & Dowling, D. K. (2020). Interactions between cytoplasmic and nuclear genomes confer sex‐specific effects on lifespan in Drosophila melanogaster. Journal of Evolutionary Biology, 33(5), 694-713. https://doi.org/10.1111/jeb.13605

Vancouver

Vaught R, Voigt S, Dobler R, Clancy D, Reinhardt K, Dowling DK. Interactions between cytoplasmic and nuclear genomes confer sex‐specific effects on lifespan in Drosophila melanogaster. Journal of Evolutionary Biology. 2020 May 1;33(5):694-713. Epub 2020 Feb 13. doi: 10.1111/jeb.13605

Author

Vaught, Rebecca ; Voigt, Suzanne ; Dobler, Ralph et al. / Interactions between cytoplasmic and nuclear genomes confer sex‐specific effects on lifespan in Drosophila melanogaster. In: Journal of Evolutionary Biology. 2020 ; Vol. 33, No. 5. pp. 694-713.

Bibtex

@article{8bba969059454f51a78cd87bdac6ddf1,
title = "Interactions between cytoplasmic and nuclear genomes confer sex‐specific effects on lifespan in Drosophila melanogaster",
abstract = "Genetic variation outside of the cell nucleus can affect the phenotype. The cytoplasm is home to the mitochondria, and in arthropods often hosts intracellular bacteria such as Wolbachia. Although numerous studies have implicated epistatic interactions between cytoplasmic and nuclear genetic variation as mediators of phenotypic expression, two questions remain. Firstly, it remains unclear whether outcomes of cyto-nuclear interactions will manifest differently across the sexes, as might be predicted given that cytoplasmic genomes are screened by natural selection only through females as a consequence of their maternal inheritance. Secondly, the relative contribution of mitochondrial genetic variation to other cytoplasmic sources of variation, such as Wolbachia infection, in shaping phenotypic outcomes of cyto-nuclear interactions remains unknown. Here, we address these questions, creating a fully crossed set of replicated cyto-nuclear populations derived from three geographically distinct populations of Drosophila melanogaster, measuring the lifespan of males and females from each population. We observed that cyto-nuclear interactions shape lifespan and that the outcomes of these interactions differ across the sexes. Yet, we found no evidence that placing the cytoplasms from one population alongside the nuclear background of others (generating putative cyto-nuclear mismatches) leads to decreased lifespan in either sex. Although it was difficult to partition mitochondrial from Wolbachia effects, our results suggest at least some of the cytoplasmic genotypic contribution to lifespan was directly mediated by an effect of sequence variation in the mtDNA. Future work should explore the degree to which cyto-nuclear interactions result in sex differences in the expression of other components of organismal life history.",
keywords = "mito-nuclear, mitonuclear, mtDNA, mitochondria, Wolbachia, Mother{\textquoteright}s Curse",
author = "Rebecca Vaught and Suzanne Voigt and Ralph Dobler and David Clancy and Klaus Reinhardt and Dowling, {Damian K}",
note = "This is the peer reviewed version of the following article: Vaught, R.C., Voigt, S., Dobler, R., Clancy, D.J., Reinhardt, K. and Dowling, D.K. (2020), Interactions between cytoplasmic and nuclear genomes confer sex‐specific effects on lifespan in Drosophila melanogaster. J Evol Biol. doi:10.1111/jeb.13605 which has been published in final form at https://onlinelibrary.wiley.com/doi/10.1111/jeb.13605 This article may be used for non-commercial purposes in accordance With Wiley Terms and Conditions for self-archiving. ",
year = "2020",
month = may,
day = "1",
doi = "10.1111/jeb.13605",
language = "English",
volume = "33",
pages = "694--713",
journal = "Journal of Evolutionary Biology",
issn = "1010-061X",
publisher = "Wiley-Blackwell",
number = "5",

}

RIS

TY - JOUR

T1 - Interactions between cytoplasmic and nuclear genomes confer sex‐specific effects on lifespan in Drosophila melanogaster

AU - Vaught, Rebecca

AU - Voigt, Suzanne

AU - Dobler, Ralph

AU - Clancy, David

AU - Reinhardt, Klaus

AU - Dowling, Damian K

N1 - This is the peer reviewed version of the following article: Vaught, R.C., Voigt, S., Dobler, R., Clancy, D.J., Reinhardt, K. and Dowling, D.K. (2020), Interactions between cytoplasmic and nuclear genomes confer sex‐specific effects on lifespan in Drosophila melanogaster. J Evol Biol. doi:10.1111/jeb.13605 which has been published in final form at https://onlinelibrary.wiley.com/doi/10.1111/jeb.13605 This article may be used for non-commercial purposes in accordance With Wiley Terms and Conditions for self-archiving.

PY - 2020/5/1

Y1 - 2020/5/1

N2 - Genetic variation outside of the cell nucleus can affect the phenotype. The cytoplasm is home to the mitochondria, and in arthropods often hosts intracellular bacteria such as Wolbachia. Although numerous studies have implicated epistatic interactions between cytoplasmic and nuclear genetic variation as mediators of phenotypic expression, two questions remain. Firstly, it remains unclear whether outcomes of cyto-nuclear interactions will manifest differently across the sexes, as might be predicted given that cytoplasmic genomes are screened by natural selection only through females as a consequence of their maternal inheritance. Secondly, the relative contribution of mitochondrial genetic variation to other cytoplasmic sources of variation, such as Wolbachia infection, in shaping phenotypic outcomes of cyto-nuclear interactions remains unknown. Here, we address these questions, creating a fully crossed set of replicated cyto-nuclear populations derived from three geographically distinct populations of Drosophila melanogaster, measuring the lifespan of males and females from each population. We observed that cyto-nuclear interactions shape lifespan and that the outcomes of these interactions differ across the sexes. Yet, we found no evidence that placing the cytoplasms from one population alongside the nuclear background of others (generating putative cyto-nuclear mismatches) leads to decreased lifespan in either sex. Although it was difficult to partition mitochondrial from Wolbachia effects, our results suggest at least some of the cytoplasmic genotypic contribution to lifespan was directly mediated by an effect of sequence variation in the mtDNA. Future work should explore the degree to which cyto-nuclear interactions result in sex differences in the expression of other components of organismal life history.

AB - Genetic variation outside of the cell nucleus can affect the phenotype. The cytoplasm is home to the mitochondria, and in arthropods often hosts intracellular bacteria such as Wolbachia. Although numerous studies have implicated epistatic interactions between cytoplasmic and nuclear genetic variation as mediators of phenotypic expression, two questions remain. Firstly, it remains unclear whether outcomes of cyto-nuclear interactions will manifest differently across the sexes, as might be predicted given that cytoplasmic genomes are screened by natural selection only through females as a consequence of their maternal inheritance. Secondly, the relative contribution of mitochondrial genetic variation to other cytoplasmic sources of variation, such as Wolbachia infection, in shaping phenotypic outcomes of cyto-nuclear interactions remains unknown. Here, we address these questions, creating a fully crossed set of replicated cyto-nuclear populations derived from three geographically distinct populations of Drosophila melanogaster, measuring the lifespan of males and females from each population. We observed that cyto-nuclear interactions shape lifespan and that the outcomes of these interactions differ across the sexes. Yet, we found no evidence that placing the cytoplasms from one population alongside the nuclear background of others (generating putative cyto-nuclear mismatches) leads to decreased lifespan in either sex. Although it was difficult to partition mitochondrial from Wolbachia effects, our results suggest at least some of the cytoplasmic genotypic contribution to lifespan was directly mediated by an effect of sequence variation in the mtDNA. Future work should explore the degree to which cyto-nuclear interactions result in sex differences in the expression of other components of organismal life history.

KW - mito-nuclear

KW - mitonuclear

KW - mtDNA

KW - mitochondria

KW - Wolbachia

KW - Mother’s Curse

U2 - 10.1111/jeb.13605

DO - 10.1111/jeb.13605

M3 - Journal article

VL - 33

SP - 694

EP - 713

JO - Journal of Evolutionary Biology

JF - Journal of Evolutionary Biology

SN - 1010-061X

IS - 5

ER -