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Interplay between Cannabinoids, SOCS3, and Autophagy Process in the Intestinal Epithelium with the Use of an Established in vitro Model System.

Research output: Thesis › Doctoral Thesis

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Interplay between Cannabinoids, SOCS3, and Autophagy Process in the Intestinal Epithelium with the Use of an Established in vitro Model System. / Koay, Luan Ching.
Lancaster: Lancaster University, 2014. 228 p.

Research output: Thesis › Doctoral Thesis

Harvard

Koay, LC 2014, 'Interplay between Cannabinoids, SOCS3, and Autophagy Process in the Intestinal Epithelium with the Use of an Established in vitro Model System.', PhD, Lancaster University, Lancaster.

APA

Koay, L. C. (2014). Interplay between Cannabinoids, SOCS3, and Autophagy Process in the Intestinal Epithelium with the Use of an Established in vitro Model System. [Doctoral Thesis, Lancaster University]. Lancaster University.

Vancouver

Koay LC. Interplay between Cannabinoids, SOCS3, and Autophagy Process in the Intestinal Epithelium with the Use of an Established in vitro Model System.. Lancaster: Lancaster University, 2014. 228 p.

Author

Koay, Luan Ching. / Interplay between Cannabinoids, SOCS3, and Autophagy Process in the Intestinal Epithelium with the Use of an Established in vitro Model System.. Lancaster : Lancaster University, 2014. 228 p.

Bibtex

@phdthesis{96b5fb4f32944cb28dbb806bb374b6c0,
title = "Interplay between Cannabinoids, SOCS3, and Autophagy Process in the Intestinal Epithelium with the Use of an Established in vitro Model System.",
abstract = "Autophagy is a catabolic process involved in homeostatic and regulated cellular protein recycling and degradation via the lysosomal degradation pathway. Emerging data associates Crohn's Disease (CD) with an impaired ATG16L1 autophagy gene. Increased activity in the endocannabinoid system and up-regulation of suppressor of cytokine signalling (SOCS)-3 protein expression are evident in inflamed intestine. We accessed the impact of phyto-cannabinoid (CBD), synthetic cannabinoid (ACEA) and endocannabinoid (AEA) on autophagosome formation, and investigated the mechanisms involved. Our findings show that all three cannabinoids induce autophagy in a dose-dependent manner in fully differentiated CaCo2 cells. ACEA and AEA induced canonical autophagy, which was cannabinoid receptor (CNR)-l mediated. In contrast, CBD-induced autophagy is partially non-canonical and not CNR1 receptor mediated. Functionally, all three cannabinoids reduce SOCS3 protein expression. Blocking of autophagy reversed the cannabinoid-induced effect. In conclusion, CBD may have potential therapeutic application in CD where functional CNR1 receptor or autophagy is compromised and the regulatory protein, SOCS3, is itself regulated by the autophagy pathway.",
keywords = "MiAaPQ, Oncology., Molecular biology.",
author = "Koay, {Luan Ching}",
note = "Thesis (Ph.D.)--Lancaster University (United Kingdom), 2014.",
year = "2014",
language = "English",
publisher = "Lancaster University",
school = "Lancaster University",

}

RIS

TY - BOOK

T1 - Interplay between Cannabinoids, SOCS3, and Autophagy Process in the Intestinal Epithelium with the Use of an Established in vitro Model System.

AU - Koay, Luan Ching

N1 - Thesis (Ph.D.)--Lancaster University (United Kingdom), 2014.

PY - 2014

Y1 - 2014

N2 - Autophagy is a catabolic process involved in homeostatic and regulated cellular protein recycling and degradation via the lysosomal degradation pathway. Emerging data associates Crohn's Disease (CD) with an impaired ATG16L1 autophagy gene. Increased activity in the endocannabinoid system and up-regulation of suppressor of cytokine signalling (SOCS)-3 protein expression are evident in inflamed intestine. We accessed the impact of phyto-cannabinoid (CBD), synthetic cannabinoid (ACEA) and endocannabinoid (AEA) on autophagosome formation, and investigated the mechanisms involved. Our findings show that all three cannabinoids induce autophagy in a dose-dependent manner in fully differentiated CaCo2 cells. ACEA and AEA induced canonical autophagy, which was cannabinoid receptor (CNR)-l mediated. In contrast, CBD-induced autophagy is partially non-canonical and not CNR1 receptor mediated. Functionally, all three cannabinoids reduce SOCS3 protein expression. Blocking of autophagy reversed the cannabinoid-induced effect. In conclusion, CBD may have potential therapeutic application in CD where functional CNR1 receptor or autophagy is compromised and the regulatory protein, SOCS3, is itself regulated by the autophagy pathway.

AB - Autophagy is a catabolic process involved in homeostatic and regulated cellular protein recycling and degradation via the lysosomal degradation pathway. Emerging data associates Crohn's Disease (CD) with an impaired ATG16L1 autophagy gene. Increased activity in the endocannabinoid system and up-regulation of suppressor of cytokine signalling (SOCS)-3 protein expression are evident in inflamed intestine. We accessed the impact of phyto-cannabinoid (CBD), synthetic cannabinoid (ACEA) and endocannabinoid (AEA) on autophagosome formation, and investigated the mechanisms involved. Our findings show that all three cannabinoids induce autophagy in a dose-dependent manner in fully differentiated CaCo2 cells. ACEA and AEA induced canonical autophagy, which was cannabinoid receptor (CNR)-l mediated. In contrast, CBD-induced autophagy is partially non-canonical and not CNR1 receptor mediated. Functionally, all three cannabinoids reduce SOCS3 protein expression. Blocking of autophagy reversed the cannabinoid-induced effect. In conclusion, CBD may have potential therapeutic application in CD where functional CNR1 receptor or autophagy is compromised and the regulatory protein, SOCS3, is itself regulated by the autophagy pathway.

KW - MiAaPQ

KW - Oncology.

KW - Molecular biology.

M3 - Doctoral Thesis

PB - Lancaster University

CY - Lancaster

ER -