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Intron-mediated gusA expression in tritordeum and wheat resulting from particle bombardment

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Intron-mediated gusA expression in tritordeum and wheat resulting from particle bombardment. / Salgueiro, Sancha; Pignocchi, Cristina; Parry, M. A J.
In: Plant Molecular Biology, Vol. 42, No. 4, 15.05.2000, p. 615-622.

Research output: Contribution to Journal/Magazine › Journal article › peer-review

Harvard

Salgueiro, S, Pignocchi, C & Parry, MAJ 2000, 'Intron-mediated gusA expression in tritordeum and wheat resulting from particle bombardment', Plant Molecular Biology, vol. 42, no. 4, pp. 615-622. https://doi.org/10.1023/A:1006331831858

APA

Salgueiro, S., Pignocchi, C., & Parry, M. A. J. (2000). Intron-mediated gusA expression in tritordeum and wheat resulting from particle bombardment. Plant Molecular Biology, 42(4), 615-622. https://doi.org/10.1023/A:1006331831858

Vancouver

Salgueiro S, Pignocchi C, Parry MAJ. Intron-mediated gusA expression in tritordeum and wheat resulting from particle bombardment. Plant Molecular Biology. 2000 May 15;42(4):615-622. doi: 10.1023/A:1006331831858

Author

Salgueiro, Sancha ; Pignocchi, Cristina ; Parry, M. A J. / Intron-mediated gusA expression in tritordeum and wheat resulting from particle bombardment. In: Plant Molecular Biology. 2000 ; Vol. 42, No. 4. pp. 615-622.

Bibtex

@article{c982097487564c8fbe779879780338d1,
title = "Intron-mediated gusA expression in tritordeum and wheat resulting from particle bombardment",
abstract = "The promoterless maize ubiquitin first exon and intron fragment can drive gusA expression in immature tritordeum inflorescences and immature wheat scutella. In fluorescence assays, this fragment induces gusA expression in tritordeum inflorescences to 50 times higher than background. The activity of the complete promoter, exon and intron cassette was up to 20 000-fold higher than background but the maize ubiquitin promoter in isolation had very low activity. A construct with the maize alcohol dehydrogenase first exon and intron had low activity, visible in histochemical assays. Both intron sequences have promoter-like features and in the ubiquitin intron there is a sequence homologous to the opaque-2-binding box. We suggest that the combination of these elements may explain the promoter activity detected in these introns.",
keywords = "Introns, Maize ubiquitin promoter, Tritordeum, Wheat",
author = "Sancha Salgueiro and Cristina Pignocchi and Parry, {M. A J}",
year = "2000",
month = may,
day = "15",
doi = "10.1023/A:1006331831858",
language = "English",
volume = "42",
pages = "615--622",
journal = "Plant Molecular Biology",
issn = "0167-4412",
publisher = "Springer Netherlands",
number = "4",

}

RIS

TY - JOUR

T1 - Intron-mediated gusA expression in tritordeum and wheat resulting from particle bombardment

AU - Salgueiro, Sancha

AU - Pignocchi, Cristina

AU - Parry, M. A J

PY - 2000/5/15

Y1 - 2000/5/15

N2 - The promoterless maize ubiquitin first exon and intron fragment can drive gusA expression in immature tritordeum inflorescences and immature wheat scutella. In fluorescence assays, this fragment induces gusA expression in tritordeum inflorescences to 50 times higher than background. The activity of the complete promoter, exon and intron cassette was up to 20 000-fold higher than background but the maize ubiquitin promoter in isolation had very low activity. A construct with the maize alcohol dehydrogenase first exon and intron had low activity, visible in histochemical assays. Both intron sequences have promoter-like features and in the ubiquitin intron there is a sequence homologous to the opaque-2-binding box. We suggest that the combination of these elements may explain the promoter activity detected in these introns.

AB - The promoterless maize ubiquitin first exon and intron fragment can drive gusA expression in immature tritordeum inflorescences and immature wheat scutella. In fluorescence assays, this fragment induces gusA expression in tritordeum inflorescences to 50 times higher than background. The activity of the complete promoter, exon and intron cassette was up to 20 000-fold higher than background but the maize ubiquitin promoter in isolation had very low activity. A construct with the maize alcohol dehydrogenase first exon and intron had low activity, visible in histochemical assays. Both intron sequences have promoter-like features and in the ubiquitin intron there is a sequence homologous to the opaque-2-binding box. We suggest that the combination of these elements may explain the promoter activity detected in these introns.

KW - Introns

KW - Maize ubiquitin promoter

KW - Tritordeum

KW - Wheat

UR - http://www.scopus.com/inward/record.url?scp=0034099777&partnerID=8YFLogxK

U2 - 10.1023/A:1006331831858

DO - 10.1023/A:1006331831858

M3 - Journal article

C2 - 10809007

AN - SCOPUS:0034099777

VL - 42

SP - 615

EP - 622

JO - Plant Molecular Biology

JF - Plant Molecular Biology

SN - 0167-4412

IS - 4

ER -