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Investigating the chemical pathway to the formation of a single biofilm using infrared spectroscopy

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Investigating the chemical pathway to the formation of a single biofilm using infrared spectroscopy. / Crisp, A.R.; Short, B.; Rowan, L. et al.
In: Biofilm, Vol. 6, 100141, 15.12.2023.

Research output: Contribution to Journal/Magazine › Journal article › peer-review

Harvard

Crisp, AR, Short, B, Rowan, L, Ramage, G, Rehman, IUR, Short, RD & Williams, C 2023, 'Investigating the chemical pathway to the formation of a single biofilm using infrared spectroscopy', Biofilm, vol. 6, 100141. https://doi.org/10.1016/j.bioflm.2023.100141

APA

Crisp, A. R., Short, B., Rowan, L., Ramage, G., Rehman, I. U. R., Short, R. D., & Williams, C. (2023). Investigating the chemical pathway to the formation of a single biofilm using infrared spectroscopy. Biofilm, 6, Article 100141. https://doi.org/10.1016/j.bioflm.2023.100141

Vancouver

Crisp AR, Short B, Rowan L, Ramage G, Rehman IUR, Short RD et al. Investigating the chemical pathway to the formation of a single biofilm using infrared spectroscopy. Biofilm. 2023 Dec 15;6:100141. Epub 2023 Jul 1. doi: 10.1016/j.bioflm.2023.100141

Author

Crisp, A.R. ; Short, B. ; Rowan, L. et al. / Investigating the chemical pathway to the formation of a single biofilm using infrared spectroscopy. In: Biofilm. 2023 ; Vol. 6.

Bibtex

@article{9f943bb178994dc0995528f6636684ed,
title = "Investigating the chemical pathway to the formation of a single biofilm using infrared spectroscopy",
abstract = "Diagnosing biofilm infections has remained a constant challenge for the last 50 years. Existing diagnostic methods struggle to identify the biofilm phenotype. Moreover, most methods of biofilm analysis destroy the biofilm making the resultant data interpretation difficult. In this study we introduce Fourier Transform Infra-Red (FTIR) spectroscopy as a label-free, non-destructive approach to monitoring biofilm progression. We have utilised FTIR in a novel application to evaluate the chemical composition of bacterial biofilms without disrupting the biofilm architecture. S. epidermidis (RP62A) was grown onto calcium fluoride slides for periods of 30 min–96 h, before semi-drying samples for analysis. We report the discovery of a chemical marker to distinguish between planktonic and biofilm samples. The appearance of new proteins in biofilm samples of varying maturity is exemplified in the spectroscopic data, highlighting the potential of FTIR for identifying the presence and developmental stage of a single biofilm.",
keywords = "Staphylococcus epidermidis, Biofilm, Infection, FTIR, Continuous monitoring, Chemical composition, Clinical diagnosis",
author = "A.R. Crisp and B. Short and L. Rowan and G. Ramage and I.U.R. Rehman and R.D. Short and C. Williams",
year = "2023",
month = dec,
day = "15",
doi = "10.1016/j.bioflm.2023.100141",
language = "English",
volume = "6",
journal = "Biofilm",
issn = "2590-2075",
publisher = "Elsevier",

}

RIS

TY - JOUR

T1 - Investigating the chemical pathway to the formation of a single biofilm using infrared spectroscopy

AU - Crisp, A.R.

AU - Short, B.

AU - Rowan, L.

AU - Ramage, G.

AU - Rehman, I.U.R.

AU - Short, R.D.

AU - Williams, C.

PY - 2023/12/15

Y1 - 2023/12/15

N2 - Diagnosing biofilm infections has remained a constant challenge for the last 50 years. Existing diagnostic methods struggle to identify the biofilm phenotype. Moreover, most methods of biofilm analysis destroy the biofilm making the resultant data interpretation difficult. In this study we introduce Fourier Transform Infra-Red (FTIR) spectroscopy as a label-free, non-destructive approach to monitoring biofilm progression. We have utilised FTIR in a novel application to evaluate the chemical composition of bacterial biofilms without disrupting the biofilm architecture. S. epidermidis (RP62A) was grown onto calcium fluoride slides for periods of 30 min–96 h, before semi-drying samples for analysis. We report the discovery of a chemical marker to distinguish between planktonic and biofilm samples. The appearance of new proteins in biofilm samples of varying maturity is exemplified in the spectroscopic data, highlighting the potential of FTIR for identifying the presence and developmental stage of a single biofilm.

AB - Diagnosing biofilm infections has remained a constant challenge for the last 50 years. Existing diagnostic methods struggle to identify the biofilm phenotype. Moreover, most methods of biofilm analysis destroy the biofilm making the resultant data interpretation difficult. In this study we introduce Fourier Transform Infra-Red (FTIR) spectroscopy as a label-free, non-destructive approach to monitoring biofilm progression. We have utilised FTIR in a novel application to evaluate the chemical composition of bacterial biofilms without disrupting the biofilm architecture. S. epidermidis (RP62A) was grown onto calcium fluoride slides for periods of 30 min–96 h, before semi-drying samples for analysis. We report the discovery of a chemical marker to distinguish between planktonic and biofilm samples. The appearance of new proteins in biofilm samples of varying maturity is exemplified in the spectroscopic data, highlighting the potential of FTIR for identifying the presence and developmental stage of a single biofilm.

KW - Staphylococcus epidermidis

KW - Biofilm

KW - Infection

KW - FTIR

KW - Continuous monitoring

KW - Chemical composition

KW - Clinical diagnosis

U2 - 10.1016/j.bioflm.2023.100141

DO - 10.1016/j.bioflm.2023.100141

M3 - Journal article

VL - 6

JO - Biofilm

JF - Biofilm

SN - 2590-2075

M1 - 100141

ER -