Home > Research > Publications & Outputs > Investigations of conformational transitions in...

Research

Associated organisational unit

Links

Text available via DOI:

Keywords

View graph of relations

Investigations of conformational transitions in proteins and RNA using 2DCOS Raman and 2DCOS Raman optical activity spectroscopies

Research output: Contribution to Journal/Magazine › Journal article › peer-review

Published

Standard

Investigations of conformational transitions in proteins and RNA using 2DCOS Raman and 2DCOS Raman optical activity spectroscopies. / Ashton, Lorna; Hobro, Alison; Conn, Graerne L. et al.
In: Journal of Molecular Structure, Vol. 883-884, 30.07.2008, p. 187-194.

Research output: Contribution to Journal/Magazine › Journal article › peer-review

Harvard

Ashton, L, Hobro, A, Conn, GL, Rouhi, M & Blanch, EW 2008, 'Investigations of conformational transitions in proteins and RNA using 2DCOS Raman and 2DCOS Raman optical activity spectroscopies', Journal of Molecular Structure, vol. 883-884, pp. 187-194. https://doi.org/10.1016/j.molstruc.2007.12.002

APA

Ashton, L., Hobro, A., Conn, G. L., Rouhi, M., & Blanch, E. W. (2008). Investigations of conformational transitions in proteins and RNA using 2DCOS Raman and 2DCOS Raman optical activity spectroscopies. Journal of Molecular Structure, 883-884, 187-194. https://doi.org/10.1016/j.molstruc.2007.12.002

Vancouver

Ashton L, Hobro A, Conn GL, Rouhi M, Blanch EW. Investigations of conformational transitions in proteins and RNA using 2DCOS Raman and 2DCOS Raman optical activity spectroscopies. Journal of Molecular Structure. 2008 Jul 30;883-884:187-194. doi: 10.1016/j.molstruc.2007.12.002

Author

Ashton, Lorna ; Hobro, Alison ; Conn, Graerne L. et al. / Investigations of conformational transitions in proteins and RNA using 2DCOS Raman and 2DCOS Raman optical activity spectroscopies. In: Journal of Molecular Structure. 2008 ; Vol. 883-884. pp. 187-194.

Bibtex

@article{480068a85437400aab9e786cbf2bb269,
title = "Investigations of conformational transitions in proteins and RNA using 2DCOS Raman and 2DCOS Raman optical activity spectroscopies",
abstract = "Coupling the structural sensitivity of vibrational spectroscopics, such as Raman scattering and Raman optical activity (ROA), with the analytical insight provided by 2D generalized correlation analysis provides a new and exciting set of tools for structural biology. These new tools of 2DCos Raman and 2DCos ROA have the potential to provide new information on the mechanisms of conformational transitions of proteins and ribonucleic acids (RNA), such as those for folding and misfolding. However, the complexity of biomolecular transitions and the practicalities of spectroscopic data collection can make the analysis of 2DCos Raman and 2DCos ROA contour plots difficult. In this paper we present it summary of our methodology for obtaining reliable 2DCos contour maps and for their interpretation for folding and misfolding transitions in polypeptides, proteins and RNA. We demonstrate that our protocols for data pre-treatment greatly improve the quality of 2DCos contour maps, revealing the large amount of structural information that they contain, and then show that a moving window analysis is required to adequately follow biomolecular conformational transitions in detail. (C) 2007 Elsevier B.V. All rights reserved.",
keywords = "2D correlation, Raman, Raman optical activity (ROA), protein folding, RNA folding, 2-DIMENSIONAL CORRELATION SPECTROSCOPY, INFRARED CORRELATION SPECTROSCOPY, DEPENDENT SPECTRAL VARIATIONS, IR CORRELATION-ANALYSIS, DYNAMIC SPECTRA, POLYMER BLENDS, SECONDARY STRUCTURE, PHASE-TRANSITIONS, MONOLAYER FILMS, POLYPROLINE-II",
author = "Lorna Ashton and Alison Hobro and Conn, {Graerne L.} and Mansour Rouhi and Blanch, {Ewan W.}",
year = "2008",
month = jul,
day = "30",
doi = "10.1016/j.molstruc.2007.12.002",
language = "English",
volume = "883-884",
pages = "187--194",
journal = "Journal of Molecular Structure",
issn = "0022-2860",
publisher = "Elsevier",
note = "4th International and 2nd Asian Symposium on Two Dimensional Correlation Spectroscopy ; Conference date: 16-08-2007 Through 18-08-2007",

}

RIS

TY - JOUR

T1 - Investigations of conformational transitions in proteins and RNA using 2DCOS Raman and 2DCOS Raman optical activity spectroscopies

AU - Ashton, Lorna

AU - Hobro, Alison

AU - Conn, Graerne L.

AU - Rouhi, Mansour

AU - Blanch, Ewan W.

PY - 2008/7/30

Y1 - 2008/7/30

N2 - Coupling the structural sensitivity of vibrational spectroscopics, such as Raman scattering and Raman optical activity (ROA), with the analytical insight provided by 2D generalized correlation analysis provides a new and exciting set of tools for structural biology. These new tools of 2DCos Raman and 2DCos ROA have the potential to provide new information on the mechanisms of conformational transitions of proteins and ribonucleic acids (RNA), such as those for folding and misfolding. However, the complexity of biomolecular transitions and the practicalities of spectroscopic data collection can make the analysis of 2DCos Raman and 2DCos ROA contour plots difficult. In this paper we present it summary of our methodology for obtaining reliable 2DCos contour maps and for their interpretation for folding and misfolding transitions in polypeptides, proteins and RNA. We demonstrate that our protocols for data pre-treatment greatly improve the quality of 2DCos contour maps, revealing the large amount of structural information that they contain, and then show that a moving window analysis is required to adequately follow biomolecular conformational transitions in detail. (C) 2007 Elsevier B.V. All rights reserved.

AB - Coupling the structural sensitivity of vibrational spectroscopics, such as Raman scattering and Raman optical activity (ROA), with the analytical insight provided by 2D generalized correlation analysis provides a new and exciting set of tools for structural biology. These new tools of 2DCos Raman and 2DCos ROA have the potential to provide new information on the mechanisms of conformational transitions of proteins and ribonucleic acids (RNA), such as those for folding and misfolding. However, the complexity of biomolecular transitions and the practicalities of spectroscopic data collection can make the analysis of 2DCos Raman and 2DCos ROA contour plots difficult. In this paper we present it summary of our methodology for obtaining reliable 2DCos contour maps and for their interpretation for folding and misfolding transitions in polypeptides, proteins and RNA. We demonstrate that our protocols for data pre-treatment greatly improve the quality of 2DCos contour maps, revealing the large amount of structural information that they contain, and then show that a moving window analysis is required to adequately follow biomolecular conformational transitions in detail. (C) 2007 Elsevier B.V. All rights reserved.

KW - 2D correlation

KW - Raman

KW - Raman optical activity (ROA)

KW - protein folding

KW - RNA folding

KW - 2-DIMENSIONAL CORRELATION SPECTROSCOPY

KW - INFRARED CORRELATION SPECTROSCOPY

KW - DEPENDENT SPECTRAL VARIATIONS

KW - IR CORRELATION-ANALYSIS

KW - DYNAMIC SPECTRA

KW - POLYMER BLENDS

KW - SECONDARY STRUCTURE

KW - PHASE-TRANSITIONS

KW - MONOLAYER FILMS

KW - POLYPROLINE-II

U2 - 10.1016/j.molstruc.2007.12.002

DO - 10.1016/j.molstruc.2007.12.002

M3 - Journal article

VL - 883-884

SP - 187

EP - 194

JO - Journal of Molecular Structure

JF - Journal of Molecular Structure

SN - 0022-2860

T2 - 4th International and 2nd Asian Symposium on Two Dimensional Correlation Spectroscopy

Y2 - 16 August 2007 through 18 August 2007

ER -