Research output: Contribution to Journal/Magazine › Journal article › peer-review
Research output: Contribution to Journal/Magazine › Journal article › peer-review
}
TY - JOUR
T1 - Investigations of conformational transitions in proteins and RNA using 2DCOS Raman and 2DCOS Raman optical activity spectroscopies
AU - Ashton, Lorna
AU - Hobro, Alison
AU - Conn, Graerne L.
AU - Rouhi, Mansour
AU - Blanch, Ewan W.
PY - 2008/7/30
Y1 - 2008/7/30
N2 - Coupling the structural sensitivity of vibrational spectroscopics, such as Raman scattering and Raman optical activity (ROA), with the analytical insight provided by 2D generalized correlation analysis provides a new and exciting set of tools for structural biology. These new tools of 2DCos Raman and 2DCos ROA have the potential to provide new information on the mechanisms of conformational transitions of proteins and ribonucleic acids (RNA), such as those for folding and misfolding. However, the complexity of biomolecular transitions and the practicalities of spectroscopic data collection can make the analysis of 2DCos Raman and 2DCos ROA contour plots difficult. In this paper we present it summary of our methodology for obtaining reliable 2DCos contour maps and for their interpretation for folding and misfolding transitions in polypeptides, proteins and RNA. We demonstrate that our protocols for data pre-treatment greatly improve the quality of 2DCos contour maps, revealing the large amount of structural information that they contain, and then show that a moving window analysis is required to adequately follow biomolecular conformational transitions in detail. (C) 2007 Elsevier B.V. All rights reserved.
AB - Coupling the structural sensitivity of vibrational spectroscopics, such as Raman scattering and Raman optical activity (ROA), with the analytical insight provided by 2D generalized correlation analysis provides a new and exciting set of tools for structural biology. These new tools of 2DCos Raman and 2DCos ROA have the potential to provide new information on the mechanisms of conformational transitions of proteins and ribonucleic acids (RNA), such as those for folding and misfolding. However, the complexity of biomolecular transitions and the practicalities of spectroscopic data collection can make the analysis of 2DCos Raman and 2DCos ROA contour plots difficult. In this paper we present it summary of our methodology for obtaining reliable 2DCos contour maps and for their interpretation for folding and misfolding transitions in polypeptides, proteins and RNA. We demonstrate that our protocols for data pre-treatment greatly improve the quality of 2DCos contour maps, revealing the large amount of structural information that they contain, and then show that a moving window analysis is required to adequately follow biomolecular conformational transitions in detail. (C) 2007 Elsevier B.V. All rights reserved.
KW - 2D correlation
KW - Raman
KW - Raman optical activity (ROA)
KW - protein folding
KW - RNA folding
KW - 2-DIMENSIONAL CORRELATION SPECTROSCOPY
KW - INFRARED CORRELATION SPECTROSCOPY
KW - DEPENDENT SPECTRAL VARIATIONS
KW - IR CORRELATION-ANALYSIS
KW - DYNAMIC SPECTRA
KW - POLYMER BLENDS
KW - SECONDARY STRUCTURE
KW - PHASE-TRANSITIONS
KW - MONOLAYER FILMS
KW - POLYPROLINE-II
U2 - 10.1016/j.molstruc.2007.12.002
DO - 10.1016/j.molstruc.2007.12.002
M3 - Journal article
VL - 883-884
SP - 187
EP - 194
JO - Journal of Molecular Structure
JF - Journal of Molecular Structure
SN - 0022-2860
T2 - 4th International and 2nd Asian Symposium on Two Dimensional Correlation Spectroscopy
Y2 - 16 August 2007 through 18 August 2007
ER -